Construct: ORF TRCN0000473616

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018511.1_s317c1
Derived from:
ccsbBroadEn_15431
DNA Barcode:
GACTTATCGCTAGTTCTTCAGTAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GNAS (2778)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473616
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2778 GNAS GNAS complex locus NM_000516.6 99.8% 100% 393C>T;1182C>T
2 human 2778 GNAS GNAS complex locus NM_001077488.4 99.5% 99.7% 257_259delGCA;396C>T;1185C>T
3 human 2778 GNAS GNAS complex locus NM_080426.4 96.2% 96.1% (many diffs)
4 human 2778 GNAS GNAS complex locus NM_001077489.4 96% 95.9% 212_213ins45;348C>T;1137C>T
5 human 2778 GNAS GNAS complex locus XM_024451872.1 88.5% 88.4% (many diffs)
6 human 2778 GNAS GNAS complex locus NM_001309840.2 84.8% 85% 0_1ins177;216C>T;1005C>T
7 human 2778 GNAS GNAS complex locus NM_001309861.2 84.8% 85% 0_1ins177;216C>T;1005C>T
8 human 2778 GNAS GNAS complex locus XM_024451873.1 84.8% 85% 0_1ins177;216C>T;1005C>T
9 human 2778 GNAS GNAS complex locus XM_024451874.1 84.8% 85% 0_1ins177;216C>T;1005C>T
10 human 2778 GNAS GNAS complex locus XM_024451875.1 84.8% 85% 0_1ins177;216C>T;1005C>T
11 human 2778 GNAS GNAS complex locus XM_017027815.1 84.8% 84.3% (many diffs)
12 human 2778 GNAS GNAS complex locus XM_017027817.1 81% 80.9% (many diffs)
13 human 2778 GNAS GNAS complex locus XM_017027818.2 81% 80.9% (many diffs)
14 human 2778 GNAS GNAS complex locus XM_017027819.1 81% 80.9% (many diffs)
15 human 2778 GNAS GNAS complex locus XM_017027816.1 80.2% 79.1% (many diffs)
16 human 2778 GNAS GNAS complex locus XM_017027820.1 80.2% 79.1% (many diffs)
17 human 2778 GNAS GNAS complex locus XR_002958471.1 52.3% (many diffs)
18 human 2778 GNAS GNAS complex locus NM_080425.3 36.7% 36% (many diffs)
19 human 2778 GNAS GNAS complex locus XM_017027812.2 36.7% 36% (many diffs)
20 human 2778 GNAS GNAS complex locus XM_017027813.2 35.4% 34.6% (many diffs)
21 human 2778 GNAS GNAS complex locus XM_017027814.2 35.3% 34.5% (many diffs)
22 human 2778 GNAS GNAS complex locus NM_001309842.2 21.9% 21.8% 258_258delAinsTG;260T>G;261_262ins920
23 mouse 14683 Gnas GNAS (guanine nucleotide bi... NM_001310083.1 95% 99.7% (many diffs)
24 mouse 14683 Gnas GNAS (guanine nucleotide bi... NM_201616.2 95% 99.7% (many diffs)
25 mouse 14683 Gnas GNAS (guanine nucleotide bi... XM_006498774.1 94.7% 99.4% (many diffs)
26 mouse 14683 Gnas GNAS (guanine nucleotide bi... NM_001077510.4 91.3% 95.9% (many diffs)
27 mouse 14683 Gnas GNAS (guanine nucleotide bi... XM_006498776.1 91.2% 95.6% (many diffs)
28 mouse 14683 Gnas GNAS (guanine nucleotide bi... XM_006498777.2 83% 87.4% (many diffs)
29 mouse 14683 Gnas GNAS (guanine nucleotide bi... XM_017315658.1 80.3% 84.7% (many diffs)
30 mouse 14683 Gnas GNAS (guanine nucleotide bi... XM_006498778.2 79.4% 83.6% (many diffs)
31 mouse 14683 Gnas GNAS (guanine nucleotide bi... NR_003258.1 64.7% (many diffs)
32 mouse 14683 Gnas GNAS (guanine nucleotide bi... NM_010309.4 31.9% 32% (many diffs)
33 mouse 14683 Gnas GNAS (guanine nucleotide bi... NM_001077507.2 30.7% 30.6% (many diffs)
34 mouse 14683 Gnas GNAS (guanine nucleotide bi... XM_006498780.1 30.6% 30.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1248
ORF length:
1182
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg ctgcctcggg aacagtaaga ccgaggacca gcgcaacgag gagaaggcgc
121agcgtgaggc caacaaaaag atcgagaagc agctgcagaa ggacaagcag gtctaccggg
181ccacgcaccg cctgctgctg ctgggtgctg gagaatctgg taaaagcacc attgtgaagc
241agatgaggat cctgcatgtt aatgggttta atggagaggg cggcgaagag gacccgcagg
301ctgcaaggag caacagcgat ggtgagaagg caaccaaagt gcaggacatc aaaaacaacc
361tgaaagaggc gattgaaacc attgtggccg ccatgagcaa cctggtgccc cccgtggagc
421tggccaaccc cgagaaccag ttcagagtgg actacattct gagtgtgatg aacgtgcctg
481actttgactt ccctcccgaa ttctatgagc atgccaaggc tctgtgggag gatgaaggag
541tgcgtgcctg ctacgaacgc tccaacgagt accagctgat tgactgtgcc cagtacttcc
601tggacaagat cgacgtgatc aagcaggctg actatgtgcc gagcgatcag gacctgcttc
661gctgccgtgt cctgacttct ggaatcTTTG AGACCAAGTT CCAGGTGGAC AAAGTCAACT
721TCCACATGTT TGACGTGGGT GGCCAGCGCG ATGAACGCCG CAAGTGGATC CAGTGCTTCA
781ACGATGTGAC TGCCATCATC TTCGTGGTGG CCAGCAGCAG CTACAACATG GTCATCCGGG
841AGGACAACCA GACCAACCGC CTGCAGGAGG CTCTGAACCT CTTCAAGAGC ATCTGGAACA
901ACAGATGGCT GCGCACCATC TCTGTGATCC TGTTCCTCAA CAAGCAAGAT CTGCTCGCTG
961AGAAAGTCCT TGCTGGGAAA TCGAAGATTG AGGACTACTT TCCAGAATTT GCTCGCTACA
1021CTACTCCTGA GGATGCTACT CCCGAGCCCG GAGAGGACCC ACGCGTGACC CGGGCCAAGT
1081ACTTCATTCG AGATGAGTTT CTGAGGATCA GCACTGCCAG TGGAGATGGG CGTCACTACT
1141GCTACCCTCA TTTCACCTGC GCTGTGGACA CTGAGAACAT CCGCCGTGTG TTCAACGACT
1201GCCGTGACAT CATTCAGCGC ATGCACCTTC GTCAGTACGA GCTGCTTTAC CCAACTTTCT
1261TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1321CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1381GTGGAAAGGA CGAGACTTAT CGCTAGTTCT TCAGTACACG CGTTAAGTCg acaatcaacc
1441tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)