Transcript: Human XM_017029873.1

PREDICTED: Homo sapiens tRNA methyltransferase 2 homolog B (TRMT2B), transcript variant X21, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TRMT2B (79979)
Length:
5502
CDS:
491..1618

Additional Resources:

NCBI RefSeq record:
XM_017029873.1
NBCI Gene record:
TRMT2B (79979)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017029873.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000275386 CATCAATGGTTACCGAAATAA pLKO_005 577 CDS 100% 15.000 21.000 N TRMT2B n/a
2 TRCN0000154263 CGGCTGACTTAACCTTAGATT pLKO.1 2214 3UTR 100% 5.625 7.875 N TRMT2B n/a
3 TRCN0000275434 CGGCTGACTTAACCTTAGATT pLKO_005 2214 3UTR 100% 5.625 7.875 N TRMT2B n/a
4 TRCN0000151234 GACTAGAGTCTTACATCCAAA pLKO.1 471 5UTR 100% 4.950 6.930 N TRMT2B n/a
5 TRCN0000158194 CCTGTCATCAATGGTTACCGA pLKO.1 572 CDS 100% 0.750 1.050 N TRMT2B n/a
6 TRCN0000154262 CATGGTGAATCCACTAGGAAT pLKO.1 1493 CDS 100% 4.950 3.465 N TRMT2B n/a
7 TRCN0000158351 CCATGGTGAATCCACTAGGAA pLKO.1 1492 CDS 100% 3.000 2.100 N TRMT2B n/a
8 TRCN0000153471 CTGGTCAAGCAGAGAAGATTT pLKO.1 1326 CDS 100% 13.200 7.920 N TRMT2B n/a
9 TRCN0000275384 CTGGTCAAGCAGAGAAGATTT pLKO_005 1326 CDS 100% 13.200 7.920 N TRMT2B n/a
10 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 2097 3UTR 100% 4.950 2.475 Y n/a
11 TRCN0000104022 GCTGTACCTGTGGATTTGTTT pLKO.1 1696 3UTR 100% 5.625 3.938 N Trmt2b n/a
12 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 4935 3UTR 100% 5.625 2.813 Y KLHL30 n/a
13 TRCN0000148469 CTGGGTTCAAGCAATTCTCTT pLKO.1 4760 3UTR 100% 4.950 2.475 Y C16orf89 n/a
14 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 4935 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017029873.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13797 pDONR223 100% 6.8% 4% None (many diffs) n/a
2 ccsbBroad304_13797 pLX_304 0% 6.8% 4% V5 (many diffs) n/a
3 TRCN0000466975 CGTACTACTGGTTGTAATCGATAG pLX_317 100% 6.8% 4% V5 (many diffs) n/a
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