Construct: ORF TRCN0000466975
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004167.1_s317c1
- Derived from:
- ccsbBroadEn_13797
- DNA Barcode:
- CGTACTACTGGTTGTAATCGATAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466975
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124801 | LSM12 | LSM12 homolog | NM_001369484.1 | 23.8% | 2.4% | (many diffs) |
| 2 | human | 51091 | SEPSECS | Sep (O-phosphoserine) tRNA:... | XM_017008277.1 | 10.5% | 2.6% | (many diffs) |
| 3 | human | 101929130 | LOC101929130 | uncharacterized LOC101929130 | NR_135551.1 | 9.4% | (many diffs) | |
| 4 | human | 83891 | SNX25 | sorting nexin 25 | XM_017008687.1 | 8.7% | 6.7% | (many diffs) |
| 5 | human | 284023 | RNF227 | ring finger protein 227 | NR_152444.1 | 7.7% | (many diffs) | |
| 6 | human | 79979 | TRMT2B | tRNA methyltransferase 2 ho... | XM_017029872.1 | 6.8% | 4% | (many diffs) |
| 7 | human | 79979 | TRMT2B | tRNA methyltransferase 2 ho... | XM_017029873.1 | 6.8% | 4% | (many diffs) |
| 8 | human | 79979 | TRMT2B | tRNA methyltransferase 2 ho... | XM_017029874.1 | 6.8% | 4% | (many diffs) |
| 9 | human | 79979 | TRMT2B | tRNA methyltransferase 2 ho... | XM_017029875.1 | 6.8% | 4% | (many diffs) |
| 10 | human | 79979 | TRMT2B | tRNA methyltransferase 2 ho... | XM_017029858.1 | 5.2% | 3.7% | (many diffs) |
| 11 | human | 79979 | TRMT2B | tRNA methyltransferase 2 ho... | XM_017029859.1 | 5.2% | 3.7% | (many diffs) |
| 12 | human | 79979 | TRMT2B | tRNA methyltransferase 2 ho... | XM_017029860.1 | 5.2% | 3.7% | (many diffs) |
| 13 | human | 79979 | TRMT2B | tRNA methyltransferase 2 ho... | XM_017029861.1 | 5.2% | 3.7% | (many diffs) |
| 14 | human | 100289019 | SLC25A25-AS1 | SLC25A25 antisense RNA 1 | NR_033374.1 | 4.8% | (many diffs) | |
| 15 | human | 23137 | SMC5 | structural maintenance of c... | XR_001746256.1 | 3.1% | (many diffs) | |
| 16 | human | 80975 | TMPRSS5 | transmembrane serine protea... | XR_001747992.1 | 2.3% | (many diffs) | |
| 17 | human | 80975 | TMPRSS5 | transmembrane serine protea... | XR_001747991.1 | 2.3% | (many diffs) | |
| 18 | human | 79815 | NIPAL2 | NIPA like domain containing 2 | XR_001745599.2 | 2.3% | (many diffs) | |
| 19 | human | 79815 | NIPAL2 | NIPA like domain containing 2 | XR_001745598.2 | 2.3% | (many diffs) | |
| 20 | human | 80975 | TMPRSS5 | transmembrane serine protea... | XR_001747990.1 | 2.3% | (many diffs) | |
| 21 | human | 114004397 | CA5BP1-CA5B | CA5BP1-CA5B readthrough | NR_160544.1 | 2.3% | (many diffs) | |
| 22 | human | 101928421 | LOC101928421 | uncharacterized LOC101928421 | XR_927171.3 | 2% | (many diffs) | |
| 23 | human | 101928421 | LOC101928421 | uncharacterized LOC101928421 | XR_927172.3 | 2% | (many diffs) | |
| 24 | human | 101928421 | LOC101928421 | uncharacterized LOC101928421 | XR_242157.4 | 2% | (many diffs) | |
| 25 | human | 23092 | ARHGAP26 | Rho GTPase activating prote... | XR_944320.3 | 2% | (many diffs) | |
| 26 | human | 23092 | ARHGAP26 | Rho GTPase activating prote... | NR_146198.2 | 2% | (many diffs) | |
| 27 | human | 23092 | ARHGAP26 | Rho GTPase activating prote... | XR_944315.3 | 1.9% | (many diffs) | |
| 28 | human | 23092 | ARHGAP26 | Rho GTPase activating prote... | XR_001742047.2 | 1.9% | (many diffs) | |
| 29 | human | 23092 | ARHGAP26 | Rho GTPase activating prote... | XR_001742048.2 | 1.9% | (many diffs) | |
| 30 | human | 23092 | ARHGAP26 | Rho GTPase activating prote... | XR_944314.3 | 1.9% | (many diffs) | |
| 31 | human | 23092 | ARHGAP26 | Rho GTPase activating prote... | XR_944311.3 | 1.8% | (many diffs) | |
| 32 | human | 101928421 | LOC101928421 | uncharacterized LOC101928421 | XR_001745169.2 | 1.6% | (many diffs) | |
| 33 | human | 101928421 | LOC101928421 | uncharacterized LOC101928421 | XR_001745168.2 | 1.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 306
- ORF length:
- 216
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGAGTCTCA CTGTATTGCC CAGGCTGGAG 121 TTCAGTGGCA CCATCTCGGC TTACTGCAGC CTCCACCTCC CGGGTTCAAG CGATTCTCCT 181 GCCTCAGCCT CCCGGGTACC TGGGGCTACA GGCGCCCGCT ACCACACCCA GCTAATTTTT 241 GTATTTTTAA TAGACATGAG GTTTCGCCAT GTTGGCCAGG CTGTTCTCGA ACTCCTGACC 301 TCAAATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 361 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 421 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACGTACTACT GGTTGTAATC GATAGACGCG 481 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt