Transcript: Mouse XM_017312231.2

PREDICTED: Mus musculus RIKEN cDNA 2310022A10 gene (2310022A10Rik), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Mus musculus (mouse)
Gene:
2310022A10Rik (66367)
Length:
2738
CDS:
188..1000

Additional Resources:

NCBI RefSeq record:
XM_017312231.2
NBCI Gene record:
2310022A10Rik (66367)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017312231.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000277446 GAGGTCAAGTTCGCCATTAAA pLKO_005 1345 3UTR 100% 15.000 21.000 N 2310022A10Rik n/a
2 TRCN0000178202 CGCTGTGATGTTTGTGGATAA pLKO.1 349 CDS 100% 10.800 8.640 N 2310022A10Rik n/a
3 TRCN0000277445 CGCTGTGATGTTTGTGGATAA pLKO_005 349 CDS 100% 10.800 8.640 N 2310022A10Rik n/a
4 TRCN0000197968 GCATGCTGCTAGATCTTAATA pLKO.1 384 CDS 100% 15.000 10.500 N 2310022A10Rik n/a
5 TRCN0000277444 AGCATGCTGCTAGATCTTAAT pLKO_005 383 CDS 100% 13.200 9.240 N 2310022A10Rik n/a
6 TRCN0000177139 GCTGTGATGTTTGTGGATAAT pLKO.1 350 CDS 100% 13.200 9.240 N 2310022A10Rik n/a
7 TRCN0000198672 CTGAGGTCAAGTTCGCCATTA pLKO.1 1343 3UTR 100% 10.800 7.560 N 2310022A10Rik n/a
8 TRCN0000197510 CATAGAACATCTGTGTTTGAT pLKO.1 910 CDS 100% 5.625 3.938 N 2310022A10Rik n/a
9 TRCN0000178543 GCGAACCTGTAAAGACTGTTA pLKO.1 2066 3UTR 100% 4.950 3.465 N 2310022A10Rik n/a
10 TRCN0000277373 GCGAACCTGTAAAGACTGTTA pLKO_005 2066 3UTR 100% 4.950 3.465 N 2310022A10Rik n/a
11 TRCN0000181641 GAAATCATGAACGAGCTGGGT pLKO.1 407 CDS 100% 0.660 0.462 N 2310022A10Rik n/a
12 TRCN0000198709 CCAGCATTTAGCTGGTTCCAA pLKO.1 1600 3UTR 100% 0.300 0.210 N 2310022A10Rik n/a
13 TRCN0000198422 GATGGAGGGAAAGTACATCAT pLKO.1 819 CDS 100% 4.950 2.970 N 2310022A10Rik n/a
14 TRCN0000177808 CCTGGAACTTGCTATGTAGAT pLKO.1 2521 3UTR 100% 4.950 2.475 Y 2310022A10Rik n/a
15 TRCN0000286002 CCTGGAACTTGCTATGTAGAT pLKO_005 2521 3UTR 100% 4.950 2.475 Y 2310022A10Rik n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017312231.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04816 pDONR223 100% 40.6% 30.7% None (many diffs) n/a
2 ccsbBroad304_04816 pLX_304 0% 40.6% 30.7% V5 (many diffs) n/a
3 TRCN0000468059 GCCCTATACCGAAGATCCTGGTTA pLX_317 29.4% 40.6% 30.7% V5 (many diffs) n/a
4 ccsbBroadEn_16085 pDONR223 0% 25.8% 14% None (many diffs) n/a
5 ccsbBroad304_16085 pLX_304 0% 25.8% 14% V5 (many diffs) n/a
6 TRCN0000473304 CTTGCCTGACGGCTTACTTCGTGG pLX_317 48.5% 25.8% 14% V5 (many diffs) n/a
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