Construct: ORF TRCN0000474127
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017603.1_s317c1
- Derived from:
- ccsbBroadEn_04150
- DNA Barcode:
- CCTCGCTTTTACACCCGGAGTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOL10 (79954)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474127
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79954 | NOL10 | nucleolar protein 10 | NM_024894.4 | 100% | 100% | |
| 2 | human | 79954 | NOL10 | nucleolar protein 10 | NM_001261392.1 | 96.2% | 96.2% | 210_211ins78 |
| 3 | human | 79954 | NOL10 | nucleolar protein 10 | NM_001261394.1 | 92.7% | 92.7% | 756_757ins150 |
| 4 | human | 79954 | NOL10 | nucleolar protein 10 | XM_011510398.3 | 78% | 77.6% | (many diffs) |
| 5 | human | 79954 | NOL10 | nucleolar protein 10 | XM_011510399.2 | 78% | 77.6% | (many diffs) |
| 6 | human | 79954 | NOL10 | nucleolar protein 10 | XR_002959342.1 | 76.4% | 1_114del;1020_1021ins67;2112_2546del | |
| 7 | human | 79954 | NOL10 | nucleolar protein 10 | XR_001738957.1 | 75.5% | 1_114del;1141_1275del;2314_2732del | |
| 8 | human | 79954 | NOL10 | nucleolar protein 10 | NR_048552.1 | 57.4% | 1_126del;190_191ins46;2145_3469del | |
| 9 | human | 79954 | NOL10 | nucleolar protein 10 | XM_011510400.2 | 51.3% | 50.7% | (many diffs) |
| 10 | human | 79954 | NOL10 | nucleolar protein 10 | XM_011510402.2 | 50% | 49.8% | (many diffs) |
| 11 | human | 79954 | NOL10 | nucleolar protein 10 | XM_024453152.1 | 44.2% | 43.8% | (many diffs) |
| 12 | human | 79954 | NOL10 | nucleolar protein 10 | XM_024453153.1 | 44.2% | 43.8% | (many diffs) |
| 13 | mouse | 217431 | Nol10 | nucleolar protein 10 | NM_001008421.1 | 88.6% | 95.4% | (many diffs) |
| 14 | mouse | 217431 | Nol10 | nucleolar protein 10 | XM_017315015.1 | 81.9% | 88.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2130
- ORF length:
- 2064
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggtctccagc ctcaatgagg tgaagattta cagcctcagc tgcggcaagt 121 cccttcctga gtggctttct gataggaaga agagagcgct acagaagaaa gatgtagatg 181 tccgtaggag aattgaactt attcaggact ttgaaatgcc tactgtgtgt accactatta 241 aggtgtcaaa agatggacag tacattttag caactggaac atataaacct cgggttcgat 301 gttatgacac ctatcaatta tccttgaagt ttgaaaggtg tttagattca gaagttgtca 361 cctttgaaat tttgtctgat gactactcaa agattgtctt cttacataat gatagataca 421 ttgaatttca ttcgcaatca ggtttttact acaaaaccag aataccaaag tttgggagag 481 atttctctta ccactatcca tcctgtgact tgtactttgt tggtgcaagt tctgaagttt 541 ataggttaaa cttagaacaa ggacgatacc tgaatcctct acaaactgat gctgcggaga 601 ataatgtttg tgacataaat tcagtgcatg gcttgtttgc cacaggaacc atagagggta 661 gagtggagtg ctgggaccca agaactcgaa acagagttgg cctgttagac tgcgccttaa 721 acagtgtcac agcagattca gagataaaca gtttaccaac aatctctgct ttgaaattta 781 atggtgcctt gaccatggca gttggaacaa ccacagggca ggttttatta tatgaccttc 841 gatctgataa gccattgcta gttaaagatc accagtatgg gctgcccatt aagtccgttc 901 atttccagga ttcattagat ctgattttgt ctgctgactc tcgaattgtc aagatgtgga 961 ataagaactc cggaaaaata tttacttcct tggagccaga gcatgacctt aatgatgttt 1021 gtctctaccc caactcaggc atgcttctga cggccaatga aacccccaag atgggcatct 1081 attacattcc agttttgggt cctgctcctc ggtggtgttc cttcttagac aacttgaccg 1141 aagaattaga agagaatcca gaaagcacag tctatgatga ttataaattt gtcaccaaga 1201 aagaccttga aaatttaggg ctcacccacc tcattggatc tcctttcctc cgggcatata 1261 tgcatgggtt tttcatggat ataagactct atcacaaggt gaaactgatg gtaaatccat 1321 ttgcttatga agaatatagg aaagataaaa tacgacagaa aatagaagaa acacgtgcac 1381 agagagtcca gttaaagaaa ttgccaaaag ttaacaaaga gctggcactt aaattaattg 1441 aggaagaaga ggagaagcag aaatctacat ggaaaaagaa agttaagagt cttcctaata 1501 ttctcaccga tgatcgattt aaagttatgt ttgagaaccc tgacttccaa gtagatgaag 1561 agagtgaaga atttaggctt ctgaatccac ttgtttcaaa aattagtgaa aaaaggaaga 1621 agaaactaag actcttagag caacaagaac ttcgtgaaaa agaagaggag gaagagccgg 1681 aaggaaaacc aagtgatgca gaaAGTTCGG AGAGTTCAGA TGATGAAAAA GCCTGGGTTG 1741 AAGAGGTCAG GAAGCAACGC AGACTCCTCC AGCAGGAGGA AAAAGTGAAG CGGCAGGAAC 1801 GACTCAAGGA GGACCAGCAG ACAGTCCTAA AGCCCCAGTT TTATGAGATC AAAGCAGGAG 1861 AAGAATTTAG AAGCTTCAAA GATTCTGCCA CAAAGCAGAA ACTGATGAAC AAAACCCTTG 1921 AAGATCGTTT GAAAATTGAA GCAAAAAATG GGACATTGAG TGTATCCGAC ACCACCGTTG 1981 GCAGCAAACA ATTGACATTC ACGTTAAAGA GGTCTGAACA GCAGAAGAAG CAACAGGAGG 2041 CTGAGAAACT GCATCGACAA GAAAGGAAAA GACTCCGTCG TTCGGCCGGA CACCTGAAGT 2101 CAAGACACAA AAGAGGACGG TCGTTTCATT GCCCAACTTT CTTGTACAAA GTGGTTGATA 2161 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2221 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCTCG 2281 CTTTTACACC CGGAGTCTAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2341 tgaaagatt