Transcript: Human XM_024449473.1

PREDICTED: Homo sapiens FERM domain containing 6 (FRMD6), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FRMD6 (122786)
Length:
4966
CDS:
400..2268

Additional Resources:

NCBI RefSeq record:
XM_024449473.1
NBCI Gene record:
FRMD6 (122786)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024449473.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000143768 CCACAGACTATATGTCGGAAA pLKO.1 1978 CDS 100% 4.050 5.670 N FRMD6 n/a
2 TRCN0000168512 GCACATTCCAAACATGCACAA pLKO.1 993 CDS 100% 4.050 3.240 N FRMD6 n/a
3 TRCN0000122114 CCTCGATGACATCAGACTTTA pLKO.1 2031 CDS 100% 13.200 9.240 N FRMD6 n/a
4 TRCN0000167279 CCCTAAACATAGCTCTTTCTT pLKO.1 2366 3UTR 100% 5.625 3.938 N FRMD6 n/a
5 TRCN0000167165 CCACCATTTCTTATGGATGAT pLKO.1 4253 3UTR 100% 4.950 3.465 N FRMD6 n/a
6 TRCN0000168430 GCCATGATTCTCAACACTGAT pLKO.1 3357 3UTR 100% 4.950 3.465 N FRMD6 n/a
7 TRCN0000142296 GCTGACTCTTGGATTGACCAT pLKO.1 1137 CDS 100% 2.640 1.848 N FRMD6 n/a
8 TRCN0000145454 GATGAAGTTCCAGAGTTTGTT pLKO.1 2242 CDS 100% 5.625 3.375 N FRMD6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024449473.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04766 pDONR223 100% 98.7% 98.7% None 270_293del n/a
2 TRCN0000492256 TTCGATCTACCATTTGATCTATAA pLX_317 25.9% 98.7% 98.7% V5 270_293del n/a
3 ccsbBroadEn_13092 pDONR223 100% 42.3% 42.4% None 1_1074del;1218G>A n/a
4 ccsbBroad304_13092 pLX_304 0% 42.3% 42.4% V5 1_1074del;1218G>A n/a
5 TRCN0000480368 TACTCACCGTCTAAGAACTCTCCT pLX_317 49.1% 42.3% 42.4% V5 1_1074del;1218G>A n/a
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