Construct: ORF TRCN0000480368
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015999.1_s317c1
- Derived from:
- ccsbBroadEn_13092
- DNA Barcode:
- TACTCACCGTCTAAGAACTCTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FRMD6 (122786)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480368
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 122786 | FRMD6 | FERM domain containing 6 | NM_001267047.1 | 99.8% | 100% | 144G>A |
| 2 | human | 122786 | FRMD6 | FERM domain containing 6 | NM_001042481.3 | 42.9% | 42.9% | 1_1050del;1194G>A |
| 3 | human | 122786 | FRMD6 | FERM domain containing 6 | NM_152330.4 | 42.9% | 42.9% | 1_1050del;1194G>A |
| 4 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_006720030.1 | 42.9% | 42.9% | 1_1050del;1194G>A |
| 5 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_024449476.1 | 42.9% | 42.9% | 1_1050del;1194G>A |
| 6 | human | 122786 | FRMD6 | FERM domain containing 6 | NM_001267046.2 | 42.3% | 42.4% | 1_1074del;1218G>A |
| 7 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_006720029.1 | 42.3% | 42.4% | 1_1074del;1218G>A |
| 8 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_011536423.1 | 42.3% | 42.4% | 1_1074del;1218G>A |
| 9 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_011536424.1 | 42.3% | 42.4% | 1_1074del;1218G>A |
| 10 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_024449472.1 | 42.3% | 42.4% | 1_1074del;1218G>A |
| 11 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_024449473.1 | 42.3% | 42.4% | 1_1074del;1218G>A |
| 12 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_024449474.1 | 42.3% | 42.4% | 1_1074del;1218G>A |
| 13 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_024449475.1 | 42.3% | 42.4% | 1_1074del;1218G>A |
| 14 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_017020968.2 | 28.8% | 27.8% | (many diffs) |
| 15 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_017020967.1 | 28.4% | 27.4% | (many diffs) |
| 16 | mouse | 319710 | Frmd6 | FERM domain containing 6 | XM_006515978.1 | 36.8% | 40.3% | (many diffs) |
| 17 | mouse | 319710 | Frmd6 | FERM domain containing 6 | XM_006515979.3 | 36.8% | 40.3% | (many diffs) |
| 18 | mouse | 319710 | Frmd6 | FERM domain containing 6 | NM_028127.3 | 36.3% | 39.8% | (many diffs) |
| 19 | mouse | 319710 | Frmd6 | FERM domain containing 6 | XM_006515977.3 | 36.3% | 39.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 858
- ORF length:
- 792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ccagctggaa aaacggtcgc gggccagcgg gagcagtgcg ggcagcatga 121 aacacaagcg cctgtcccgt cattccaccg ccagccacag cagttcccac acctcgggca 181 ttgaggcaga caccaagccc cgggacacag ggccagaaga cagctactcc agcagtgcca 241 tccaccgcaa gctgaaaacc tgcagctcaa tgaccagtca tggcagctcc cacacctcag 301 gggtggagag tggcggcaaa gaccggctgg aagaggactt acaggacgat gaaatagaga 361 tgttggttga tgacccccgg gatctggagc agatgaatga agagtctctg gaagtcagcc 421 cagacatgtg catctacatc acagaggaca tgctcatgtc gcggaagctG AATGGACACT 481 CTGGGTTGAT TGTGAAAGAA ATTGGGTCTT CCACCTCGAG CTCTTCAGAA ACAGTTGTTA 541 AGCTTCGTGG CCAGAGTACT GATTCTCTTC CACAGACTAT ATGTCGGAAA CCAAAGACCT 601 CCACTGATCG ACACAGCTTG AGCCTCGATG ACATCAGACT TTACCAGAAA GACTTCCTGC 661 GCATTGCAGG TCTGTGTCAG GACACTGCTC AGAGTTACAC CTTTGGATGT GGCCATGAAC 721 TGGATGAGGA AGGCCTCTAT TGCAACAGTT GCTTGGCCCA GCAGTGCATC AACATCCAAG 781 ATGCTTTTCC AGTCAAAAGA ACCAGCAAAT ACTTTTCTCT GGATCTCACT CATGATGAAG 841 TTCCAGAGTT TGTTGTGAAC CCAACTTACT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATACTCAC CGTCTAAGAA 1021 CTCTCCTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt