Construct: ORF TRCN0000492256
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009282.2_s317c1
- Derived from:
- ccsbBroadEn_04766
- DNA Barcode:
- TTCGATCTACCATTTGATCTATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FRMD6 (122786)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492256
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 122786 | FRMD6 | FERM domain containing 6 | NM_001042481.3 | 100% | 100% | |
| 2 | human | 122786 | FRMD6 | FERM domain containing 6 | NM_152330.4 | 100% | 100% | |
| 3 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_006720030.1 | 100% | 100% | |
| 4 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_024449476.1 | 100% | 100% | |
| 5 | human | 122786 | FRMD6 | FERM domain containing 6 | NM_001267046.2 | 98.7% | 98.7% | 270_293del |
| 6 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_006720029.1 | 98.7% | 98.7% | 270_293del |
| 7 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_011536423.1 | 98.7% | 98.7% | 270_293del |
| 8 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_011536424.1 | 98.7% | 98.7% | 270_293del |
| 9 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_024449472.1 | 98.7% | 98.7% | 270_293del |
| 10 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_024449473.1 | 98.7% | 98.7% | 270_293del |
| 11 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_024449474.1 | 98.7% | 98.7% | 270_293del |
| 12 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_024449475.1 | 98.7% | 98.7% | 270_293del |
| 13 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_017020968.2 | 85.8% | 84.8% | (many diffs) |
| 14 | human | 122786 | FRMD6 | FERM domain containing 6 | XM_017020967.1 | 84.7% | 83.7% | (many diffs) |
| 15 | human | 122786 | FRMD6 | FERM domain containing 6 | NM_001267047.1 | 42.9% | 42.9% | 0_1ins1050 |
| 16 | mouse | 319710 | Frmd6 | FERM domain containing 6 | XM_006515978.1 | 86.1% | 93.4% | (many diffs) |
| 17 | mouse | 319710 | Frmd6 | FERM domain containing 6 | XM_006515979.3 | 86.1% | 93.4% | (many diffs) |
| 18 | mouse | 319710 | Frmd6 | FERM domain containing 6 | NM_028127.3 | 84.9% | 92.2% | (many diffs) |
| 19 | mouse | 319710 | Frmd6 | FERM domain containing 6 | XM_006515977.3 | 84.9% | 92.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1908
- ORF length:
- 1842
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa caaattgaat tttcataaca acagagtcat gcaagaccgc cgcagtgtgt 121 gcattttcct tcccaacgat gaatctctga acatcatcat aaatgttaag attctgtgtc 181 accagttgct ggtccaggtt tgtgacctgc tcaggctaaa ggactgccac ctctttggac 241 tcagtgttat acaaaataat gaacatgtgt atatggagtt gtcacaaaag ctttacaaat 301 attgtccaaa agaatggaag aaagaggcca gcaagggtat cgaccaattt gggcctccta 361 tgatcatcca cttccgtgtg cagtactatg tggaaaatgg cagattgatc agtgacagag 421 cagcaagata ctattattac tggcacctga gaaaacaagt tcttcattct cagtgtgtgc 481 tccgagagga ggcctacttc ctgctggcag cctttgccct gcaggctgat cttgggaact 541 tcaaaaggaa taagcactat ggaaaatact tcgagccaga ggcttacttc ccatcttggg 601 ttgtttccaa gagggggaag gactacatcc tgaagcacat tccaaacatg cacaaagatc 661 agtttgcact aacagcttcc gaagctcatc ttaaatatat caaagaggct gtccgactgg 721 atgacgtcgc tgttcattac tacagattgt ataaggataa aagggaaatt gaagcatcgc 781 tgactcttgg attgaccatg aggggaatac agatttttca gaatttagat gaagagaaac 841 aattacttta tgatttcccc tggacaaatg ttggaaaatt ggtgtttgtg ggtaagaaat 901 ttgagatttt gccagatggc ttgccttctg cccggaagct catatactac acggggtgcc 961 ccatgcgctc cagacacctc ctgcaacttc tgagcaacag ccaccgcctc tatatgaatc 1021 tgcagcctgt cctgcgccat atccggaagc tggaggaaaa cgaagagaag aagcagtacc 1081 gggaatctta catcagtgac aacctggacc tcgacatgga ccagctggaa aaacggtcgc 1141 gggccagcgg gagcagtgcg ggcagcatga aacacaagcg cctgtcccgt cattccaccg 1201 ccagccacag cagttcccac acctcgggca ttgaggcaga caccaagccc cgggacacgg 1261 ggccagaaga cagctactcc agcagtgcca tccaccgcaa gctgaaaacc tgcagctcaa 1321 tgaccagtca tggcagctcc cacacctcag gggtggagag tggcggcaaa gaccggctgg 1381 aagaggactt acaggacgat gaaatagaga tgttggttga tgacccccgg gatctGGAGC 1441 AGATGAATGA AGAGTCTCTG GAAGTCAGCC CAGACATGTG CATCTACATC ACAGAGGACA 1501 TGCTCATGTC GCGGAAGCTG AATGGACACT CTGGGTTGAT TGTGAAAGAA ATTGGGTCTT 1561 CCACCTCGAG CTCTTCAGAA ACAGTTGTTA AGCTTCGTGG CCAGAGTACT GATTCTCTTC 1621 CACAGACTAT ATGTCGGAAA CCAAAGACCT CCACTGATCG ACACAGCTTG AGCCTCGATG 1681 ACATCAGACT TTACCAGAAA GACTTCCTGC GCATTGCAGG TCTGTGTCAG GACACTGCTC 1741 AGAGTTACAC CTTTGGATGT GGCCATGAAC TGGATGAGGA AGGCCTCTAT TGCAACAGTT 1801 GCTTGGCCCA GCAGTGCATC AACATCCAAG ATGCTTTTCC AGTCAAAAGA ACCAGCAAAT 1861 ACTTTTCTCT GGATCTCACT CATGATGAAG TTCCAGAGTT TGTTGTGTAC CCAACTTTCT 1921 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1981 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2041 GTGGAAAGGA CGATTCGATC TACCATTTGA TCTATAAACG CGTTAAGTCg acaatcaacc 2101 tctggattac aaaatttgtg aaagatt