Transcript: Human XR_001743731.2

PREDICTED: Homo sapiens ring finger protein 8 (RNF8), transcript variant X4, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RNF8 (9025)
Length:
2156
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001743731.2
NBCI Gene record:
RNF8 (9025)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001743731.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000293325 TACATGTCGAAAGAGTTATTT pLKO_005 1925 3UTR 100% 15.000 21.000 N RNF8 n/a
2 TRCN0000293324 TGGAACCTTTAAGGGTCTATT pLKO_005 457 3UTR 100% 13.200 18.480 N RNF8 n/a
3 TRCN0000003440 CAAAGAATTAGAGCAGACCAA pLKO.1 1289 3UTR 100% 2.640 3.696 N RNF8 n/a
4 TRCN0000003441 CCAAAGAATGACCAAATGATA pLKO.1 594 3UTR 100% 5.625 3.938 N RNF8 n/a
5 TRCN0000298501 CCAAAGAATGACCAAATGATA pLKO_005 594 3UTR 100% 5.625 3.938 N RNF8 n/a
6 TRCN0000003438 TGGAGCAACTAGAGAAGACTT pLKO.1 1111 3UTR 100% 4.950 3.465 N RNF8 n/a
7 TRCN0000293306 TGGAGCAACTAGAGAAGACTT pLKO_005 1111 3UTR 100% 4.950 3.465 N RNF8 n/a
8 TRCN0000003439 GAAGCCGTTATGAATGTGAAA pLKO.1 984 3UTR 100% 4.950 2.970 N RNF8 n/a
9 TRCN0000293390 GAAGCCGTTATGAATGTGAAA pLKO_005 984 3UTR 100% 4.950 2.970 N RNF8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001743731.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07340 pDONR223 100% 67.4% None (many diffs) n/a
2 ccsbBroad304_07340 pLX_304 0% 67.4% V5 (many diffs) n/a
3 TRCN0000467155 CCCTCTGAGTGTACCATCTCGAAG pLX_317 25.9% 67.4% V5 (many diffs) n/a
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