Construct: ORF ccsbBroad304_07340
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011661.1_s304c1, BRDN0000410112
- Derived from:
- ccsbBroadEn_07340
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF8 (9025)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07340
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9025 | RNF8 | ring finger protein 8 | NM_003958.4 | 99.9% | 100% | 1344G>A |
| 2 | human | 9025 | RNF8 | ring finger protein 8 | XM_006715241.3 | 93.7% | 93.8% | 1036_1037ins90;1254G>A |
| 3 | human | 9025 | RNF8 | ring finger protein 8 | NM_183078.3 | 88.9% | 82.5% | (many diffs) |
| 4 | human | 9025 | RNF8 | ring finger protein 8 | XM_017011462.1 | 88.1% | 88.2% | 0_1ins171;1173G>A |
| 5 | human | 9025 | RNF8 | ring finger protein 8 | XM_006715242.3 | 82.8% | 76.5% | (many diffs) |
| 6 | human | 9025 | RNF8 | ring finger protein 8 | XM_017011463.1 | 77.3% | 71.1% | (many diffs) |
| 7 | human | 9025 | RNF8 | ring finger protein 8 | XM_017011464.1 | 73.8% | 65.9% | (many diffs) |
| 8 | human | 9025 | RNF8 | ring finger protein 8 | XR_427853.3 | 71.4% | (many diffs) | |
| 9 | human | 9025 | RNF8 | ring finger protein 8 | XR_001743731.2 | 67.4% | (many diffs) | |
| 10 | human | 9025 | RNF8 | ring finger protein 8 | XR_001743734.2 | 64.9% | (many diffs) | |
| 11 | human | 9025 | RNF8 | ring finger protein 8 | NR_046399.2 | 25.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1521
- ORF length:
- 1455
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ggagcccggc ttcttcgtca caggagaccg cgccggtggc cggagctggt 121 gcctgcggcg ggtggggatg agcgccgggt ggctgctgct ggaagatggg tgcgaggtga 181 ctgtaggacg aggatttggt gtcacatacc aactggtatc aaaaatctgc cccctgatga 241 tttctcgaaa ccactgtgtt ttgaagcaga atcctgaggg ccaatggaca attatggaca 301 acaagagtct aaatggtgtt tggctgaaca gagcgcgtct ggaaccttta agggtctatt 361 ccattcatca gggagactac atccaacttg gagtgcctct ggaaaataag gagaatgcgg 421 agtatgaata tgaagttact gaagaagact gggagacaat atatccttgt ctttccccaa 481 agaatgacca aatgatagaa aaaaataagg aattgagaac taaaaggaaa ttcagtttgg 541 atgaattagc aggtcctgga gctgaaggcc cctcaaattt gaaatccaaa ataaataaag 601 tgtcttgtga atctggtcag ccagtgaaat cacaggggaa aggtgaagtg gccagtacac 661 cctctgacaa tttggatcct aagttgactg cccttgagcc aagtaagacc acaggggctc 721 ccatttaccc tggcttcccc aaagtcacag aggttcatca tgagcagaaa gcctcaaact 781 cttcagcatc tcagagaagc ttacagatgt ttaaggtgac catgtccagg attctgaggc 841 tcaaaataca gatgcaggaa aaacatgaag ccgttatgaa tgtgaaaaag cagacccaaa 901 aggggaactc aaagaaagtt gtgcaaatgg agcaggaact tcaggactta cagtcccagc 961 tgtgtgcaga gcaggctcag cagcaggcaa gagtggagca actagagaag actttccagg 1021 aagaggaaca gcatcttcag ggtttggaga tagcccaagg agaaaaggac ctgaagcaac 1081 agctggccca ggctctgcag gagcattggg ctctaatgga agagctaaat cgcagcaaga 1141 aggactttga agcaatcatt caagccaaga acaaagaatt agagcagacc aaggaagaga 1201 aggagaagat gcaagcacag aaggaagaag ttcttagcca catgaatgat gtgctagaga 1261 atgagctcca atgtattatt tgttcagaat acttcattga ggctgtcacc ttgaactgtg 1321 cccacagttt ctgctcctac tgtatcaatg aatggatgaa gcggaagata gaatgcccca 1381 tttgtcggaa ggacattaag tccaaaacat actctttggt tctggacaat tgcattaata 1441 agatggtaaa taatctgagc tcagaagtga aagaacgacg aattgttctc attagggaac 1501 gaaaagcaaa gagattgttc tgcccaactt tcttgtacaa agtggttggt aagcctatcc 1561 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1621 ttaagtcgac aatcaacctc tggatta