Transcript: Human XR_001747099.2

PREDICTED: Homo sapiens HPS1 biogenesis of lysosomal organelles complex 3 subunit 1 (HPS1), transcript variant X6, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
HPS1 (3257)
Length:
2899
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001747099.2
NBCI Gene record:
HPS1 (3257)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001747099.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000381345 GCTTGGTGCACTTCATCTATG pLKO_005 1866 3UTR 100% 10.800 15.120 N HPS1 n/a
2 TRCN0000380407 GACGGTCATCTTATCCGAAAG pLKO_005 645 3UTR 100% 6.000 8.400 N HPS1 n/a
3 TRCN0000382212 AGCACCTGGCTGGAGTTTAAG pLKO_005 1605 3UTR 100% 13.200 10.560 N HPS1 n/a
4 TRCN0000379764 ACTGGACAGATCAGGAGTTTG pLKO_005 316 3UTR 100% 10.800 7.560 N HPS1 n/a
5 TRCN0000379465 ACTTCCTGTGGTTCGAGAATG pLKO_005 2079 3UTR 100% 10.800 7.560 N HPS1 n/a
6 TRCN0000379770 CCATGGATGCCCTTCAGATAG pLKO_005 1240 3UTR 100% 10.800 7.560 N HPS1 n/a
7 TRCN0000382402 CCTTCACCTGTTTGGAGAATG pLKO_005 515 3UTR 100% 10.800 7.560 N HPS1 n/a
8 TRCN0000381812 GCCAGAGGATGGACAAGTTTG pLKO_005 1555 3UTR 100% 10.800 7.560 N HPS1 n/a
9 TRCN0000082871 GTGCTCAAGTACCTGTTTGAA pLKO.1 600 3UTR 100% 5.625 3.938 N HPS1 n/a
10 TRCN0000082870 CCTGTGGTTCGAGAATGACAT pLKO.1 2083 3UTR 100% 4.950 3.465 N HPS1 n/a
11 TRCN0000201507 CCTGTGGTTCGAGAATGACAT pLKO.1 2083 3UTR 100% 4.950 3.465 N Hps1 n/a
12 TRCN0000082868 GCTCAATAAATGTTGGTCATT pLKO.1 2861 3UTR 100% 4.950 3.465 N HPS1 n/a
13 TRCN0000082869 GTCCTCTTCTACTGGACAGAT pLKO.1 306 3UTR 100% 4.950 3.465 N HPS1 n/a
14 TRCN0000082872 CCTGTATGTCCTTCACCTGTT pLKO.1 506 3UTR 100% 4.050 2.835 N HPS1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001747099.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06399 pDONR223 100% 33.2% None (many diffs) n/a
2 ccsbBroad304_06399 pLX_304 0% 33.2% V5 (many diffs) n/a
3 TRCN0000466587 AAGCTAGGACAAGGATACTCGCAC pLX_317 30% 33.2% V5 (many diffs) n/a
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