Transcript: Human XR_002957039.1

PREDICTED: Homo sapiens ankyrin repeat domain 30A (ANKRD30A), transcript variant X2, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ANKRD30A (91074)
Length:
10233
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002957039.1
NBCI Gene record:
ANKRD30A (91074)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002957039.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000365468 CAGTCCTAGCATCACCTTATG pLKO_005 4743 3UTR 100% 10.800 15.120 N ANKRD30A n/a
2 TRCN0000365471 TGCGGAATGAAAGTTTCTATT pLKO_005 1868 3UTR 100% 13.200 17.160 N ANKRD30A n/a
3 TRCN0000022239 CCTGCGGAATGAAAGTTTCTA pLKO.1 1866 3UTR 100% 5.625 7.313 N ANKRD30A n/a
4 TRCN0000365467 ACGTCTTTGCTGCAGATATAT pLKO_005 723 3UTR 100% 15.000 10.500 N ANKRD30A n/a
5 TRCN0000370725 AGACACACCTAGGGAAATTAT pLKO_005 1201 3UTR 100% 15.000 10.500 N ANKRD30A n/a
6 TRCN0000365469 TAGATTCTGGTGCCGATATAA pLKO_005 411 3UTR 100% 15.000 10.500 N ANKRD30A n/a
7 TRCN0000370724 ACTGAAAGAGGAATCATTAAC pLKO_005 3928 3UTR 100% 13.200 9.240 N ANKRD30A n/a
8 TRCN0000365470 ATCAGGAGGCTTGTGCAAATA pLKO_005 384 3UTR 100% 13.200 9.240 N ANKRD30A n/a
9 TRCN0000370723 CATCACATTCATGAACAAATT pLKO_005 785 3UTR 100% 13.200 9.240 N ANKRD30A n/a
10 TRCN0000022241 CTGCGGAATGAAAGTTTCTAT pLKO.1 1867 3UTR 100% 5.625 3.938 N ANKRD30A n/a
11 TRCN0000022242 GATGAGATACTCCCATCAGAA pLKO.1 2036 3UTR 100% 4.950 3.465 N ANKRD30A n/a
12 TRCN0000022240 GCTGCAGATATATGTGGAGTA pLKO.1 731 3UTR 100% 4.050 2.835 N ANKRD30A n/a
13 TRCN0000022243 GCGAATGCAGTTAATAAGTAT pLKO.1 626 3UTR 100% 5.625 3.375 N ANKRD30A n/a
14 TRCN0000043501 GCTTGGACTAAATTCAGTCAT pLKO.1 8225 3UTR 100% 4.950 2.475 Y ATP8A2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002957039.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10303 pDONR223 100% 1.7% None (many diffs) n/a
2 ccsbBroad304_10303 pLX_304 0% 1.7% V5 (many diffs) n/a
3 TRCN0000476207 CTCACACCATTTCATCTACCCCAA pLX_317 100% 1.7% V5 (many diffs) n/a
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