Construct: ORF TRCN0000467879

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013883.1_s317c1
Derived from:
ccsbBroadEn_02088
DNA Barcode:
TATACCCTGATCCCCTGATACGTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRPF3 (9129)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467879
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9129 PRPF3 pre-mRNA processing factor 3 NM_004698.4 100% 100%
2 human 9129 PRPF3 pre-mRNA processing factor 3 XR_921998.3 86.4% 1_94del;1296_1297insTACAG;2139_2360del
3 human 9129 PRPF3 pre-mRNA processing factor 3 XR_002958012.1 86% (many diffs)
4 human 9129 PRPF3 pre-mRNA processing factor 3 XR_001737537.2 83.7% (many diffs)
5 human 9129 PRPF3 pre-mRNA processing factor 3 NR_146768.1 81.9% 1_173del;2016_2025delGGTGAATGGG;2233_2501del
6 human 9129 PRPF3 pre-mRNA processing factor 3 XR_241103.3 81.6% 1_94del;1733_1734ins119;2025_2246del
7 human 9129 PRPF3 pre-mRNA processing factor 3 XR_921997.3 80.6% (many diffs)
8 human 9129 PRPF3 pre-mRNA processing factor 3 NR_146766.1 80.3% 1_173del;1700_1757del;2281_2549del
9 human 9129 PRPF3 pre-mRNA processing factor 3 NM_001350529.1 80.2% 80.2% 0_1ins405
10 human 9129 PRPF3 pre-mRNA processing factor 3 NR_146769.1 79.8% (many diffs)
11 human 9129 PRPF3 pre-mRNA processing factor 3 XR_001737541.2 79.5% 1_94del;1129_1130ins167;1977_2198del
12 human 9129 PRPF3 pre-mRNA processing factor 3 XR_001737536.2 79% (many diffs)
13 human 9129 PRPF3 pre-mRNA processing factor 3 XM_011510130.3 78.9% 78.9% 0_1ins432
14 human 9129 PRPF3 pre-mRNA processing factor 3 XM_017002790.1 78.9% 78.9% 0_1ins432
15 human 9129 PRPF3 pre-mRNA processing factor 3 NR_146767.1 77.4% (many diffs)
16 human 9129 PRPF3 pre-mRNA processing factor 3 XR_002958009.1 69.5% 1_599del;2126_2183del;2707_2946del
17 human 9129 PRPF3 pre-mRNA processing factor 3 XM_011510131.3 62.8% 62.3% (many diffs)
18 human 9129 PRPF3 pre-mRNA processing factor 3 XM_011510132.3 60.9% 59% 1202_1203insTACAG;1248_1249ins796
19 human 9129 PRPF3 pre-mRNA processing factor 3 XR_001737540.2 59.8% (many diffs)
20 human 9129 PRPF3 pre-mRNA processing factor 3 XM_017002791.2 53% 51.2% 1035_1036ins167;1086_1087ins796
21 human 9129 PRPF3 pre-mRNA processing factor 3 XR_002958010.1 48.8% (many diffs)
22 mouse 70767 Prpf3 pre-mRNA processing factor 3 NM_001316751.1 92.1% 99.5% (many diffs)
23 mouse 70767 Prpf3 pre-mRNA processing factor 3 NM_027541.4 92.1% 99.5% (many diffs)
24 mouse 70767 Prpf3 pre-mRNA processing factor 3 XM_006502080.3 74.3% 79.5% (many diffs)
25 mouse 70767 Prpf3 pre-mRNA processing factor 3 XM_006502081.2 74% 79.7% (many diffs)
26 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_375580.2 56% (many diffs)
27 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_375581.2 55.2% (many diffs)
28 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_001783748.1 54.9% (many diffs)
29 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_001783747.1 54% (many diffs)
30 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_001783749.1 53.7% (many diffs)
31 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_001783750.1 53.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2115
ORF length:
2049
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc actgtcaaag agggagctgg atgagctgaa accatggata gagaagacag
121tgaagagggt cctgggtttc tcagagccta cggtggtcac agcagcattg aactgtgtgg
181ggaagggcat ggacaagaag aaggcagccg atcatctgaa accttttctt gatgattcta
241ctctccgatt tgtggacaaa ctgtttgagg ctgtggagga aggccgaagc tctaggcatt
301ccaagtctag cagtgacagg agcagaaaac gagagctaaa ggaggtgttt ggtgatgact
361ctgagatctc taaagaatca tcaggagtaa agaagcgacg aataccccgt tttgaggagg
421tggaagaaga gccagaggtg atccctgggc ctccatcaga gagccctggc atgctgacta
481agctccagat caaacagatg atggaggcag caacacgaca aatcgaggag aggaaaaaac
541agctgagctt cattagcccc cctacacctc agccaaagac tccttcttcc tcccaaccag
601aacgacttcc tattggcaac actattcagc cctcccaggc tgccactttc atgaatgatg
661ccattgagaa ggcaaggaaa gcagctgaac tgcaagctcg aatccaagcc cagctggcac
721tgaagccagg actcatcggc aatgccaaca tggtgggcct ggctaatctc catgccatgg
781gcattgctcc cccgaaggtg gagttaaaag accaaacgaa acctacacca ctgatcctgg
841atgagcaagg gcgcactgta gatgcaacag gcaaggagat tgagctgaca caccgcatgc
901ctactctgaa agccaatatt cgtgctgtga agagggaaca attcaagcaa caactaaagg
961aaaagccatc agaagacatg gaatccaata ccttttttga cccccgagtc tccattgccc
1021cttcccagcg ccagagacgc acttttaaat tccatgacaa gggcaaattt gagaagattg
1081ctcagcgatt acggacaaag gctcaactgg agaagctaca ggcagagatt tcacaagcag
1141ctcgaaaaac aggcatccat acttcgacta ggcttgccct cattgctcct aagaaggagc
1201taaaggaagg agatattcct gaaattgagt ggtgggactc ttacataatc cccaatggct
1261ttgatcttac agaggaaaat cccaagagag aagattattt tggaatcaca aatcttgttg
1321aacatccagc ccagctcaat cctccagttg acaatgacac accagttact ctgggagtat
1381atcttaccaa gaaggaacag aaaaaacttc ggagacaaac aaggagggaa gcacagaagg
1441aactacaaga aaaagtcagg ctgggcctga tgcctcctcc agaacccaaa gtgagaattt
1501ctaatttgat gcgagtatta ggaacagaag ctgttcaaga ccccacgaag gtagaagccc
1561acgtcagagc tcagatggca aaaagacaga aagcgcatga agaggccaac gctgcccgaa
1621aactcacagc agaacagaga aaggtcaaga aaattaaaaa gcttaaagaa gacatttcac
1681agggggtaca catatctgta tatagagttc gaaatttgag caacccagcc aagaagttca
1741agattgaagc caaTGCTGGG CAACTGTACC TGACAGGGGT GGTGGTACTG CACAAGGATG
1801TCAACGTGGT AGTAGTGGAA GGGGGCCCCA AGGCCCAGAA GAAATTTAAG CGTCTTATGC
1861TGCATCGGAT AAAGTGGGAT GAACAGACAT CTAACACAAA GGGAGATGAT GATGAGGAGT
1921CTGATGAGGA AGCTGTGAAG AAAACCAACA AATGTGTACT AGTCTGGGAG GGTACAGCCA
1981AAGACCGGAG CTTTGGAGAG ATGAAGTTTA AACAGTGTCC TACAGAGAAC ATGGCTCGTG
2041AGCATTTCAA AAAGCATGGG GCTGAACACT ACTGGGACCT TGCGCTGAGT GAATCTGTGT
2101TAGAGTCCAC TGATTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
2161CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
2221TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TATACCCTGA TCCCCTGATA
2281CGTGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.