Construct: ORF TRCN0000467879
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013883.1_s317c1
- Derived from:
- ccsbBroadEn_02088
- DNA Barcode:
- TATACCCTGATCCCCTGATACGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRPF3 (9129)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467879
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | NM_004698.4 | 100% | 100% | |
| 2 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | XR_921998.3 | 86.4% | 1_94del;1296_1297insTACAG;2139_2360del | |
| 3 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | XR_002958012.1 | 86% | (many diffs) | |
| 4 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | XR_001737537.2 | 83.7% | (many diffs) | |
| 5 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | NR_146768.1 | 81.9% | 1_173del;2016_2025delGGTGAATGGG;2233_2501del | |
| 6 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | XR_241103.3 | 81.6% | 1_94del;1733_1734ins119;2025_2246del | |
| 7 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | XR_921997.3 | 80.6% | (many diffs) | |
| 8 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | NR_146766.1 | 80.3% | 1_173del;1700_1757del;2281_2549del | |
| 9 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | NM_001350529.1 | 80.2% | 80.2% | 0_1ins405 |
| 10 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | NR_146769.1 | 79.8% | (many diffs) | |
| 11 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | XR_001737541.2 | 79.5% | 1_94del;1129_1130ins167;1977_2198del | |
| 12 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | XR_001737536.2 | 79% | (many diffs) | |
| 13 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | XM_011510130.3 | 78.9% | 78.9% | 0_1ins432 |
| 14 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | XM_017002790.1 | 78.9% | 78.9% | 0_1ins432 |
| 15 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | NR_146767.1 | 77.4% | (many diffs) | |
| 16 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | XR_002958009.1 | 69.5% | 1_599del;2126_2183del;2707_2946del | |
| 17 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | XM_011510131.3 | 62.8% | 62.3% | (many diffs) |
| 18 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | XM_011510132.3 | 60.9% | 59% | 1202_1203insTACAG;1248_1249ins796 |
| 19 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | XR_001737540.2 | 59.8% | (many diffs) | |
| 20 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | XM_017002791.2 | 53% | 51.2% | 1035_1036ins167;1086_1087ins796 |
| 21 | human | 9129 | PRPF3 | pre-mRNA processing factor 3 | XR_002958010.1 | 48.8% | (many diffs) | |
| 22 | mouse | 70767 | Prpf3 | pre-mRNA processing factor 3 | NM_001316751.1 | 92.1% | 99.5% | (many diffs) |
| 23 | mouse | 70767 | Prpf3 | pre-mRNA processing factor 3 | NM_027541.4 | 92.1% | 99.5% | (many diffs) |
| 24 | mouse | 70767 | Prpf3 | pre-mRNA processing factor 3 | XM_006502080.3 | 74.3% | 79.5% | (many diffs) |
| 25 | mouse | 70767 | Prpf3 | pre-mRNA processing factor 3 | XM_006502081.2 | 74% | 79.7% | (many diffs) |
| 26 | mouse | 70767 | Prpf3 | pre-mRNA processing factor 3 | XR_375580.2 | 56% | (many diffs) | |
| 27 | mouse | 70767 | Prpf3 | pre-mRNA processing factor 3 | XR_375581.2 | 55.2% | (many diffs) | |
| 28 | mouse | 70767 | Prpf3 | pre-mRNA processing factor 3 | XR_001783748.1 | 54.9% | (many diffs) | |
| 29 | mouse | 70767 | Prpf3 | pre-mRNA processing factor 3 | XR_001783747.1 | 54% | (many diffs) | |
| 30 | mouse | 70767 | Prpf3 | pre-mRNA processing factor 3 | XR_001783749.1 | 53.7% | (many diffs) | |
| 31 | mouse | 70767 | Prpf3 | pre-mRNA processing factor 3 | XR_001783750.1 | 53.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2115
- ORF length:
- 2049
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc actgtcaaag agggagctgg atgagctgaa accatggata gagaagacag 121 tgaagagggt cctgggtttc tcagagccta cggtggtcac agcagcattg aactgtgtgg 181 ggaagggcat ggacaagaag aaggcagccg atcatctgaa accttttctt gatgattcta 241 ctctccgatt tgtggacaaa ctgtttgagg ctgtggagga aggccgaagc tctaggcatt 301 ccaagtctag cagtgacagg agcagaaaac gagagctaaa ggaggtgttt ggtgatgact 361 ctgagatctc taaagaatca tcaggagtaa agaagcgacg aataccccgt tttgaggagg 421 tggaagaaga gccagaggtg atccctgggc ctccatcaga gagccctggc atgctgacta 481 agctccagat caaacagatg atggaggcag caacacgaca aatcgaggag aggaaaaaac 541 agctgagctt cattagcccc cctacacctc agccaaagac tccttcttcc tcccaaccag 601 aacgacttcc tattggcaac actattcagc cctcccaggc tgccactttc atgaatgatg 661 ccattgagaa ggcaaggaaa gcagctgaac tgcaagctcg aatccaagcc cagctggcac 721 tgaagccagg actcatcggc aatgccaaca tggtgggcct ggctaatctc catgccatgg 781 gcattgctcc cccgaaggtg gagttaaaag accaaacgaa acctacacca ctgatcctgg 841 atgagcaagg gcgcactgta gatgcaacag gcaaggagat tgagctgaca caccgcatgc 901 ctactctgaa agccaatatt cgtgctgtga agagggaaca attcaagcaa caactaaagg 961 aaaagccatc agaagacatg gaatccaata ccttttttga cccccgagtc tccattgccc 1021 cttcccagcg ccagagacgc acttttaaat tccatgacaa gggcaaattt gagaagattg 1081 ctcagcgatt acggacaaag gctcaactgg agaagctaca ggcagagatt tcacaagcag 1141 ctcgaaaaac aggcatccat acttcgacta ggcttgccct cattgctcct aagaaggagc 1201 taaaggaagg agatattcct gaaattgagt ggtgggactc ttacataatc cccaatggct 1261 ttgatcttac agaggaaaat cccaagagag aagattattt tggaatcaca aatcttgttg 1321 aacatccagc ccagctcaat cctccagttg acaatgacac accagttact ctgggagtat 1381 atcttaccaa gaaggaacag aaaaaacttc ggagacaaac aaggagggaa gcacagaagg 1441 aactacaaga aaaagtcagg ctgggcctga tgcctcctcc agaacccaaa gtgagaattt 1501 ctaatttgat gcgagtatta ggaacagaag ctgttcaaga ccccacgaag gtagaagccc 1561 acgtcagagc tcagatggca aaaagacaga aagcgcatga agaggccaac gctgcccgaa 1621 aactcacagc agaacagaga aaggtcaaga aaattaaaaa gcttaaagaa gacatttcac 1681 agggggtaca catatctgta tatagagttc gaaatttgag caacccagcc aagaagttca 1741 agattgaagc caaTGCTGGG CAACTGTACC TGACAGGGGT GGTGGTACTG CACAAGGATG 1801 TCAACGTGGT AGTAGTGGAA GGGGGCCCCA AGGCCCAGAA GAAATTTAAG CGTCTTATGC 1861 TGCATCGGAT AAAGTGGGAT GAACAGACAT CTAACACAAA GGGAGATGAT GATGAGGAGT 1921 CTGATGAGGA AGCTGTGAAG AAAACCAACA AATGTGTACT AGTCTGGGAG GGTACAGCCA 1981 AAGACCGGAG CTTTGGAGAG ATGAAGTTTA AACAGTGTCC TACAGAGAAC ATGGCTCGTG 2041 AGCATTTCAA AAAGCATGGG GCTGAACACT ACTGGGACCT TGCGCTGAGT GAATCTGTGT 2101 TAGAGTCCAC TGATTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2161 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2221 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TATACCCTGA TCCCCTGATA 2281 CGTGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt