Construct: ORF ccsbBroad304_02088

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013883.1_s304c1, BRDN0000409256
Derived from:
ccsbBroadEn_02088
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRPF3 (9129)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02088
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9129 PRPF3 pre-mRNA processing factor 3 NM_004698.4 100% 100%
2 human 9129 PRPF3 pre-mRNA processing factor 3 XR_921998.3 86.4% 1_94del;1296_1297insTACAG;2139_2360del
3 human 9129 PRPF3 pre-mRNA processing factor 3 XR_002958012.1 86% (many diffs)
4 human 9129 PRPF3 pre-mRNA processing factor 3 XR_001737537.2 83.7% (many diffs)
5 human 9129 PRPF3 pre-mRNA processing factor 3 NR_146768.1 81.9% 1_173del;2016_2025delGGTGAATGGG;2233_2501del
6 human 9129 PRPF3 pre-mRNA processing factor 3 XR_241103.3 81.6% 1_94del;1733_1734ins119;2025_2246del
7 human 9129 PRPF3 pre-mRNA processing factor 3 XR_921997.3 80.6% (many diffs)
8 human 9129 PRPF3 pre-mRNA processing factor 3 NR_146766.1 80.3% 1_173del;1700_1757del;2281_2549del
9 human 9129 PRPF3 pre-mRNA processing factor 3 NM_001350529.1 80.2% 80.2% 0_1ins405
10 human 9129 PRPF3 pre-mRNA processing factor 3 NR_146769.1 79.8% (many diffs)
11 human 9129 PRPF3 pre-mRNA processing factor 3 XR_001737541.2 79.5% 1_94del;1129_1130ins167;1977_2198del
12 human 9129 PRPF3 pre-mRNA processing factor 3 XR_001737536.2 79% (many diffs)
13 human 9129 PRPF3 pre-mRNA processing factor 3 XM_011510130.3 78.9% 78.9% 0_1ins432
14 human 9129 PRPF3 pre-mRNA processing factor 3 XM_017002790.1 78.9% 78.9% 0_1ins432
15 human 9129 PRPF3 pre-mRNA processing factor 3 NR_146767.1 77.4% (many diffs)
16 human 9129 PRPF3 pre-mRNA processing factor 3 XR_002958009.1 69.5% 1_599del;2126_2183del;2707_2946del
17 human 9129 PRPF3 pre-mRNA processing factor 3 XM_011510131.3 62.8% 62.3% (many diffs)
18 human 9129 PRPF3 pre-mRNA processing factor 3 XM_011510132.3 60.9% 59% 1202_1203insTACAG;1248_1249ins796
19 human 9129 PRPF3 pre-mRNA processing factor 3 XR_001737540.2 59.8% (many diffs)
20 human 9129 PRPF3 pre-mRNA processing factor 3 XM_017002791.2 53% 51.2% 1035_1036ins167;1086_1087ins796
21 human 9129 PRPF3 pre-mRNA processing factor 3 XR_002958010.1 48.8% (many diffs)
22 mouse 70767 Prpf3 pre-mRNA processing factor 3 NM_001316751.1 92.1% 99.5% (many diffs)
23 mouse 70767 Prpf3 pre-mRNA processing factor 3 NM_027541.4 92.1% 99.5% (many diffs)
24 mouse 70767 Prpf3 pre-mRNA processing factor 3 XM_006502080.3 74.3% 79.5% (many diffs)
25 mouse 70767 Prpf3 pre-mRNA processing factor 3 XM_006502081.2 74% 79.7% (many diffs)
26 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_375580.2 56% (many diffs)
27 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_375581.2 55.2% (many diffs)
28 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_001783748.1 54.9% (many diffs)
29 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_001783747.1 54% (many diffs)
30 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_001783749.1 53.7% (many diffs)
31 mouse 70767 Prpf3 pre-mRNA processing factor 3 XR_001783750.1 53.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2115
ORF length:
2049
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc actgtcaaag agggagctgg atgagctgaa accatggata gagaagacag
121tgaagagggt cctgggtttc tcagagccta cggtggtcac agcagcattg aactgtgtgg
181ggaagggcat ggacaagaag aaggcagccg atcatctgaa accttttctt gatgattcta
241ctctccgatt tgtggacaaa ctgtttgagg ctgtggagga aggccgaagc tctaggcatt
301ccaagtctag cagtgacagg agcagaaaac gagagctaaa ggaggtgttt ggtgatgact
361ctgagatctc taaagaatca tcaggagtaa agaagcgacg aataccccgt tttgaggagg
421tggaagaaga gccagaggtg atccctgggc ctccatcaga gagccctggc atgctgacta
481agctccagat caaacagatg atggaggcag caacacgaca aatcgaggag aggaaaaaac
541agctgagctt cattagcccc cctacacctc agccaaagac tccttcttcc tcccaaccag
601aacgacttcc tattggcaac actattcagc cctcccaggc tgccactttc atgaatgatg
661ccattgagaa ggcaaggaaa gcagctgaac tgcaagctcg aatccaagcc cagctggcac
721tgaagccagg actcatcggc aatgccaaca tggtgggcct ggctaatctc catgccatgg
781gcattgctcc cccgaaggtg gagttaaaag accaaacgaa acctacacca ctgatcctgg
841atgagcaagg gcgcactgta gatgcaacag gcaaggagat tgagctgaca caccgcatgc
901ctactctgaa agccaatatt cgtgctgtga agagggaaca attcaagcaa caactaaagg
961aaaagccatc agaagacatg gaatccaata ccttttttga cccccgagtc tccattgccc
1021cttcccagcg ccagagacgc acttttaaat tccatgacaa gggcaaattt gagaagattg
1081ctcagcgatt acggacaaag gctcaactgg agaagctaca ggcagagatt tcacaagcag
1141ctcgaaaaac aggcatccat acttcgacta ggcttgccct cattgctcct aagaaggagc
1201taaaggaagg agatattcct gaaattgagt ggtgggactc ttacataatc cccaatggct
1261ttgatcttac agaggaaaat cccaagagag aagattattt tggaatcaca aatcttgttg
1321aacatccagc ccagctcaat cctccagttg acaatgacac accagttact ctgggagtat
1381atcttaccaa gaaggaacag aaaaaacttc ggagacaaac aaggagggaa gcacagaagg
1441aactacaaga aaaagtcagg ctgggcctga tgcctcctcc agaacccaaa gtgagaattt
1501ctaatttgat gcgagtatta ggaacagaag ctgttcaaga ccccacgaag gtagaagccc
1561acgtcagagc tcagatggca aaaagacaga aagcgcatga agaggccaac gctgcccgaa
1621aactcacagc agaacagaga aaggtcaaga aaattaaaaa gcttaaagaa gacatttcac
1681agggggtaca catatctgta tatagagttc gaaatttgag caacccagcc aagaagttca
1741agattgaagc caatgctggg caactgtacc tgacaggggt ggtggtactg cacaaggatg
1801tcaacgtggt agtagtggaa gggggcccca aggcccagaa gaaatttaag cgtcttatgc
1861tgcatcggat aaagtgggat gaacagacat ctaacacaaa gggagatgat gatgaggagt
1921ctgatgagga agctgtgaag aaaaccaaca aatgtgtact agtctgggag ggtacagcca
1981aagaccggag ctttggagag atgaagttta aacagtgtcc tacagagaac atggctcgtg
2041agcatttcaa aaagcatggg gctgaacact actgggacct tgcgctgagt gaatctgtgt
2101tagagtccac tgattgccca actttcttgt acaaagtggt tggtaagcct atccctaacc
2161ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
2221cgacaatcaa cctctggatt a

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