Transcript: Human NM_001286199.1

Homo sapiens SR-related CTD associated factor 8 (SCAF8), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
SCAF8 (22828)
Length:
4897
CDS:
233..4048

Additional Resources:

NCBI RefSeq record:
NM_001286199.1
NBCI Gene record:
SCAF8 (22828)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001286199.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000352984 GCCGTGCCTACAGTTAGTTTA pLKO_005 2174 CDS 100% 13.200 18.480 N SCAF8 n/a
2 TRCN0000344138 TTACCTCCAATTAGATCTAAA pLKO_005 1622 CDS 100% 13.200 18.480 N SCAF8 n/a
3 TRCN0000155701 CGTGGGTATCTCAGATAACAA pLKO.1 762 CDS 100% 5.625 7.875 N SCAF8 n/a
4 TRCN0000154945 GCCAGCAGCTTCAACAATTAA pLKO.1 831 CDS 100% 15.000 10.500 N SCAF8 n/a
5 TRCN0000344137 TATTCCCTGAATGACTATAAA pLKO_005 266 CDS 100% 15.000 10.500 N SCAF8 n/a
6 TRCN0000352985 CTTTAGTGCAGCCGTCATTAT pLKO_005 2373 CDS 100% 13.200 9.240 N SCAF8 n/a
7 TRCN0000344193 CATTAGGTTGGATATGGTAAT pLKO_005 4367 3UTR 100% 10.800 7.560 N SCAF8 n/a
8 TRCN0000339193 TGACTCCATTGTGCGACAATC pLKO_005 430 CDS 100% 10.800 7.560 N Scaf8 n/a
9 TRCN0000156141 CGACATCAGTTTGGTCAAGAA pLKO.1 452 CDS 100% 4.950 3.465 N SCAF8 n/a
10 TRCN0000155947 CCAGTACAGAATGATCCTGAA pLKO.1 3917 CDS 100% 4.050 2.835 N SCAF8 n/a
11 TRCN0000156109 CCAAATTACTAAGGCAGCCAT pLKO.1 313 CDS 100% 2.640 1.848 N SCAF8 n/a
12 TRCN0000155923 CCAAGGTCATTAAGATCGCTT pLKO.1 1854 CDS 100% 2.640 1.848 N SCAF8 n/a
13 TRCN0000152996 GCTCAAGATCAAGAACACATT pLKO.1 1422 CDS 100% 4.950 2.970 N SCAF8 n/a
14 TRCN0000153537 CGTTTCAGAATCTGTGAACAT pLKO.1 1102 CDS 100% 4.950 3.465 N SCAF8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001286199.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07805 pDONR223 100% 99.9% 99.9% None 890A>T;3048C>T n/a
2 ccsbBroad304_07805 pLX_304 0% 99.9% 99.9% V5 890A>T;3048C>T n/a
3 TRCN0000470849 GCCGAACTCATGCTACGTGTAGAC pLX_317 9.3% 99.9% 99.9% V5 890A>T;3048C>T n/a
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