Construct: sgRNA BRDN0001147422

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACATCCCTGAAGAGTAACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2K5 (5607)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77265

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 67543450 - MAP2K5 NNNTCCCTGAAGAGTAACTG NGG 0 1.0 Tier I
2 NC_000012.12 12 30711080 - CAPRIN2 NNNTCACTGAAGAGTAACTT NGG 2 0.65 Tier II
3 NC_000022.11 22 31282338 + PIK3IP1 NNNTCCCTGAAGAGAAACTG NGG 1 0.5789 Tier II
4 NC_000005.10 5 96489301 + CAST NNNTGCCTGAAGAGTAACTT NGG 2 0.42 Tier II
5 NC_000023.11 X 13902462 - GPM6B NNNTCCCTGAAGAATAAATG NGG 2 0.4038 Tier II
6 NC_000017.11 17 1737279 + WDR81 NNNTCCCTGGAGAGAAACTG NGG 2 0.3216 Tier II
7 NC_000003.12 3 71485483 + FOXP1 NNNTCCCTGAATAATAACTG NGG 2 0.2885 Tier II
8 NC_000017.11 17 63910604 - CSHL1 NNNTCCCAGAAGAGTAACAG NGG 2 0.2286 Tier II
9 NC_000007.14 7 141184342 - TMEM178B NNNTCCCTAAAGAGTAACAG NGG 2 0.1837 Tier II
10 NC_000001.11 1 204451650 - PIK3C2B NNNTCCCTGATGAGAAACTG NGG 2 0.1781 Tier II
11 NC_000022.11 22 41504415 - ACO2 NNNTCCCTGAGGATTAACTG NGG 2 0.1733 Tier II
12 NC_000005.10 5 137477678 - SPOCK1 NNNTCCCTGGAGAGTAAGTG NGG 2 0.0741 Tier II
13 NC_000012.12 12 26651612 - ITPR2 NNNTCTCTGAAGAGTAACTG NGA 2 0.0645 Tier II
14 NC_000003.12 3 52345376 - DNAH1 NNNTCCCTGAAGAGTGACTG NCG 2 0.0206 Tier II
15 NC_000002.12 2 197203129 - ANKRD44 NNNTCCCTGAAGAGGGACTG NGG 2 0.0096 Tier II
16 NC_000007.14 7 141214560 - TMEM178B NNNTCCCTGAAGAGTATCTG NGC 2 0.003 Tier II
17 NC_000005.10 5 141506114 + PCDHGC3 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
18 NC_000005.10 5 141506114 + PCDHGB4 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
19 NC_000005.10 5 141506114 + PCDHGA8 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
20 NC_000005.10 5 141506114 + PCDHGA12 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
21 NC_000005.10 5 141506114 + PCDHGC5 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
22 NC_000005.10 5 141506114 + PCDHGC4 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
23 NC_000005.10 5 141506114 + PCDHGB7 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
24 NC_000005.10 5 141506114 + PCDHGB6 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
25 NC_000005.10 5 141506114 + PCDHGB5 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
26 NC_000005.10 5 141506114 + PCDHGB3 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
27 NC_000005.10 5 141506114 + PCDHGB2 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
28 NC_000005.10 5 141506114 + PCDHGB1 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
29 NC_000005.10 5 141506114 + PCDHGA11 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
30 NC_000005.10 5 141506114 + PCDHGA10 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
31 NC_000005.10 5 141506114 + PCDHGA9 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
32 NC_000005.10 5 141506114 + PCDHGA7 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
33 NC_000005.10 5 141506114 + PCDHGA6 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
34 NC_000005.10 5 141506114 + PCDHGA5 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
35 NC_000005.10 5 141506114 + PCDHGA4 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
36 NC_000005.10 5 141506114 + PCDHGA3 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
37 NC_000005.10 5 141506114 + PCDHGA2 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
38 NC_000005.10 5 141506114 + PCDHGA1 NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier II
39 NC_000004.12 4 108089496 - LEF1 NNNTCCCTGAAGAGTCACTG NTG 2 0.0 Tier II
40 NC_000006.12 6 33802868 + MLN NNNTCCCTGAAGAGTCTCTG NGG 2 0.0 Tier II
41 NC_000015.10 15 59650397 - GTF2A2 NNNTCCCTGAAGAGTTACTG NGA 2 0.0 Tier II
42 NC_000002.12 2 33080691 + LTBP1 NNNTCCCTGAATAGTTACTG NGG 2 0.0 Tier II
43 NC_000023.11 X 104794503 - IL1RAPL2 NNNTCCCTGAGGAGTCACTG NGG 2 0.0 Tier II
44 NC_000014.9 14 69333385 - GALNT16 NNNTCCCTGCAGAGTCACTG NGG 2 0.0 Tier II
45 NC_000015.10 15 85582165 + AKAP13 NNNTCCCTTAAGAGTCACTG NGG 2 0.0 Tier II
46 NC_000003.12 3 5973733 - LOC102723596 NNNTCTCTGAAGAATAACTG NGG 2 0.6964 Tier III
47 NC_000006.12 6 90723301 - LOC107986623 NNNTACTTGAAGAGTAACTG NGG 2 0.4643 Tier III
48 NC_000007.14 7 131870211 + LOC107986849 NNNCCCCTGAGGAGTAACTG NGG 2 0.4206 Tier III
49 NC_000005.10 5 96489301 + LOC101929710 NNNTGCCTGAAGAGTAACTT NGG 2 0.42 Tier III
50 NC_000005.10 5 96489301 + LOC102724070 NNNTGCCTGAAGAGTAACTT NGG 2 0.42 Tier III
51 NC_000003.12 3 150597561 + LOC124900547 NNNCCCCTGAAGAGAAACTG NGG 2 0.3746 Tier III
52 NC_000008.11 8 37521938 - LINC01605 NNNACCCTGAAGGGTAACTG NGG 2 0.3106 Tier III
53 NC_000014.9 14 106442388 + IGH NNNTCCCTGAAGGGCAACTG NGG 2 0.1779 Tier III
54 NC_000014.9 14 106442388 + LOC124903400 NNNTCCCTGAAGGGCAACTG NGG 2 0.1779 Tier III
55 NC_000017.11 17 70830855 + LOC105371884 NNNTTCCTGAAGAGTAGCTG NGG 2 0.1123 Tier III
56 NC_000002.12 2 129460788 - LOC105373613 NNNTCCCTGCAGAGTGACTG NGG 2 0.0641 Tier III
57 NC_000004.12 4 186019524 + LOC124900830 NNNTCCCTGAAGAGTAAATG NGC 2 0.012 Tier III
58 NC_000004.12 4 180512122 - LOC105377565 NNNTCCCTGAAGACTAACTG NGT 2 0.0069 Tier III
59 NC_000004.12 4 180512122 - LOC105377567 NNNTCCCTGAAGACTAACTG NGT 2 0.0069 Tier III
60 NC_000005.10 5 141506114 + PCDHG@ NNNTCCCTGAAGAGTCACTA NGG 2 0.0 Tier III
61 NC_000006.12 6 33802868 + LOC105375024 NNNTCCCTGAAGAGTCTCTG NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 63377144 + Map2k5 NNNTCCCTGAAGAGTAACTG NGG 0 1.0 Tier I
2 NC_000067.6 1 52596038 - Nemp2 NNNTCACTGTAGAGTAACTG NGG 2 0.8193 Tier II
3 NC_000067.6 1 11460134 - A830018L16Rik NNNTCCTTGAAGAGTAACTA NGG 2 0.7617 Tier II
4 NC_000078.6 12 106026624 + Vrk1 NNNTCCCAGAAGAATAACTG NGG 2 0.6 Tier II
5 NC_000080.6 14 120277973 - Mbnl2 NNNTCCCTGAAGAGAAACTG NGG 1 0.5789 Tier II
6 NC_000076.6 10 76458499 - 2610028H24Rik NNNGCCCTGTAGAGTAACTG NGG 2 0.5515 Tier II
7 NC_000076.6 10 76458499 - Ybey NNNGCCCTGTAGAGTAACTG NGG 2 0.5515 Tier II
8 NC_000074.6 8 76905023 + Nr3c2 NNNTCCCTGTAGAGAAACTG NGG 2 0.5108 Tier II
9 NC_000073.6 7 99232291 - Mogat2 NNNTCACTGAAGAGTAAATG NGG 2 0.5 Tier II
10 NC_000077.6 11 118567233 + Rbfox3 NNNCCCCTGAAGAATAACTG NGG 2 0.4853 Tier II
11 NC_000072.6 6 15266753 - Foxp2 NNNACCCTGTAGAGTAACTG NGG 2 0.4202 Tier II
12 NC_000068.7 2 10191292 + Itih5 NNNTTCCTGAAGAGAAACTG NGG 2 0.3684 Tier II
13 NC_000083.6 17 5431222 + Tmem242 NNNTCTCTGCAGAGTAACTG NGG 2 0.3095 Tier II
14 NC_000079.6 13 73757247 - Slc12a7 NNNTCCTTGCAGAGTAACTG NGG 2 0.2708 Tier II
15 NC_000077.6 11 43120177 + Atp10b NNNTCTCTGAAGAGTAACTG NAG 2 0.2407 Tier II
16 NC_000071.6 5 122744195 - Camkk2 NNNTCCCTGAAGAATAACAG NGG 2 0.2143 Tier II
17 NC_000082.6 16 34454627 - Kalrn NNNTCCATGAAGAGTAACTG NAG 2 0.1944 Tier II
18 NC_000085.6 19 55302955 - Zdhhc6 NNNTCCCTGATGAGAAACTG NGG 2 0.1781 Tier II
19 NC_000068.7 2 24705644 + Cacna1b NNNTCCCTGAACTGTAACTG NGG 2 0.1588 Tier II
20 NC_000073.6 7 96548637 + Tenm4 NNNTCCTTGAAGAGTGACTG NGG 2 0.1563 Tier II
21 NC_000071.6 5 122001302 + Cux2 NNNTCCCTGAAGAGTAAAGG NGG 2 0.1346 Tier II
22 NC_000068.7 2 105244955 - Them7 NNNTCCCTGAGGAGTGACTG NGG 2 0.125 Tier II
23 NC_000069.6 3 129608623 - Elovl6 NNNTCTCTGAAGAGTATCTG NGG 2 0.1238 Tier II
24 NC_000067.6 1 153083791 - Nmnat2 NNNTCCCAGAAGAGTAAGTG NGG 2 0.1067 Tier II
25 NC_000083.6 17 24529655 + Rab26 NNNTCCCTGAAGAGCAACAG NGG 2 0.0779 Tier II
26 NC_000073.6 7 55141077 - Luzp2 NNNTCCCTGAAGAGAATCTG NGG 2 0.0772 Tier II
27 NC_000072.6 6 94133120 - Magi1 NNNTCCCTGCAGAGTACCTG NGG 2 0.0588 Tier II
28 NC_000086.7 X 51672729 - Hs6st2 NNNTCCCTGAAGACTATCTG NGG 2 0.0571 Tier II
29 NC_000068.7 2 162645890 + Ptprt NNNTCCCTGAAGAGTAAATG NGA 2 0.0374 Tier II
30 NC_000077.6 11 47817922 + Sgcd NNNCCCCTGAAGAGGAACTG NGG 2 0.0324 Tier II
31 NC_000071.6 5 131065479 + Galnt17 NNNTCCCTGAAGAGGAAATG NGG 2 0.0269 Tier II
32 NC_000078.6 12 77324330 + Fut8 NNNTCCCTGAACAGGAACTG NGG 2 0.0265 Tier II
33 NC_000068.7 2 84812537 + Smtnl1 NNNTCCCTCAAGAGTAACTG NTG 2 0.021 Tier II
34 NC_000071.6 5 90350893 + Ankrd17 NNNTCCATGAAGAGTAACTG NGT 2 0.0121 Tier II
35 NC_000083.6 17 43290911 - Adgrf1 NNNTCCCTGAAGATTTACTG NGG 2 0.0 Tier II
36 NC_000071.6 5 119824129 + Tbx5 NNNTCCCTGAGGAGTCACTG NGG 2 0.0 Tier II
37 NC_000080.6 14 120277973 - Gm52122 NNNTCCCTGAAGAGAAACTG NGG 1 0.5789 Tier III
38 NC_000075.6 9 47352025 + Gm31816 NNNTCCCTGAATAGTAACTT NGG 2 0.2692 Tier III
39 NC_000068.7 2 144911488 - Gm39952 NNNTCCCTGAAGTGAAACTG NGG 2 0.1737 Tier III
40 NC_000072.6 6 94133120 - 4930511A08Rik NNNTCCCTGCAGAGTACCTG NGG 2 0.0588 Tier III
41 NC_000079.6 13 15830808 - B230303A05Rik NNNTCCCTGATGAGTAACTG NGA 2 0.0214 Tier III
42 NC_000071.6 5 119824129 + Gm5563 NNNTCCCTGAGGAGTCACTG NGG 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)