GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001148862

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: GCCCCGCCGCCCTCCCCTCC

Original Target:

Taxon:: Homo sapiens (human)
Gene:: Negative control (unspec'd)
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 80263

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000019.10	19	11178453	-	KANK2	NNNCCGCTGTCCTCCCCTCC	NGG	2	0.8235	Tier I
2	NC_000017.11	17	42680181	-	CCR10	NNNCCGCGGCCCTCCACTCC	NGG	2	0.6429	Tier I
3	NC_000009.12	9	35075735	-	FANCG	NNNCCACCCCCCTCCCCTCC	NGG	2	0.5385	Tier I
4	NC_000004.12	4	159355893	+	RAPGEF2	NNNCCCCCGCCCACCCCTCC	NGG	2	0.472	Tier I
5	NC_000008.11	8	142475569	-	ADGRB1	NNNCCGCCGCCCTCCACGCC	NGG	2	0.3333	Tier I
6	NC_000003.12	3	52488214	+	NISCH	NNNCCGCCGCCATCCCCTAC	NGG	2	0.3061	Tier I
7	NC_000022.11	22	39679383	-	CACNA1I	NNNCCGCCGCCCGCCCCTCC	NGG	1	0.2609	Tier I
8	NC_000007.14	7	100586105	+	LRCH4	NNNCCGCCGCCATCCGCTCC	NGG	2	0.1099	Tier I
9	NC_000001.11	1	201982927	-	RNPEP	NNNCCGCCGCCGTCACCTCC	NGG	2	0.0988	Tier I
10	NC_000017.11	17	17138049	+	MPRIP	NNNCCGCCGCTCTCCCCTCC	NAG	2	0.0798	Tier I
11	NC_000019.10	19	45940296	-	NOVA2	NNNCCGCCGCCCCCGCCTCC	NGG	2	0.0395	Tier I
12	NC_000009.12	9	135087392	-	OLFM1	NNNCCGCGGCCCTCCCGTCC	NGG	2	0.0378	Tier I
13	NC_000007.14	7	65873561	+	VKORC1L1	NNNCCGCCGCCCTTGCCTCC	NGG	2	0.0367	Tier I
14	NC_000017.11	17	44354435	+	FAM171A2	NNNCCGCCGCCCTCGCCCCC	NGG	2	0.0214	Tier I
15	NC_000011.10	11	78962283	-	TENM4	NNNCCGCCCCCCTCCCCTCC	NGC	2	0.012	Tier I
16	NC_000019.10	19	1229843	-	CBARP	NNNCCGCCGCCCTCCCCGCC	NGC	2	0.0074	Tier I
17	NC_000019.10	19	16324998	-	KLF2	NNNCCGCCGCCCTCACCTCC	NGC	2	0.0049	Tier I
18	NC_000019.10	19	31344729	-	TSHZ3	NNNCCGCCGCCCTCCACTCC	NGG	1	1.0	Tier II
19	NC_000003.12	3	115147123	+	ZBTB20	NNNCCGCCGCCCCCCACTCC	NGG	2	0.7895	Tier II
20	NC_000012.12	12	103495483	-	C12orf42	NNNCCGCCGCCCTCCCCACC	NGG	1	0.6667	Tier II
21	NC_000019.10	19	50567167	-	LRRC4B	NNNCCTCCGCCCTCCCCTCC	NGG	1	0.6667	Tier II
22	NC_000017.11	17	77433787	+	SEPTIN9	NNNCCCCTGCCCTCCCCTCC	NGG	2	0.5966	Tier II
23	NC_000019.10	19	30491564	+	ZNF536	NNNCCCCTGCCCTCCCCTCC	NGG	2	0.5966	Tier II
24	NC_000023.11	X	153818654	-	PDZD4	NNNCCTTCGCCCTCCCCTCC	NGG	2	0.5417	Tier II
25	NC_000005.10	5	178616188	+	CLK4	NNNCCGCCTTCCTCCCCTCC	NGG	2	0.502	Tier II
26	NC_000009.12	9	127115945	+	RALGPS1	NNNCAGCTGCCCTCCCCTCC	NGG	2	0.5	Tier II
27	NC_000009.12	9	127115945	+	ANGPTL2	NNNCAGCTGCCCTCCCCTCC	NGG	2	0.5	Tier II
28	NC_000001.11	1	17692622	+	ARHGEF10L	NNNCCGCCGCCCTTCCCACC	NGG	2	0.4889	Tier II
29	NC_000008.11	8	22103015	+	FHIP2B	NNNCCGCCACACTCCCCTCC	NGG	2	0.4821	Tier II
30	NC_000016.10	16	27482251	-	GTF3C1	NNNCCGCCACACTCCCCTCC	NGG	2	0.4821	Tier II
31	NC_000017.11	17	80001493	-	TBC1D16	NNNCCCCCGCCCACCCCTCC	NGG	2	0.472	Tier II
32	NC_000017.11	17	48551311	+	HOXB3	NNNCCGCCGCCCTCCCCTTC	NGG	1	0.4615	Tier II
33	NC_000013.11	13	51451766	-	INTS6	NNNCCACCGCCGTCCCCTCC	NGG	2	0.4444	Tier II
34	NC_000001.11	1	5873164	+	NPHP4	NNNCCCCAGCCCTCCCCTCC	NGG	2	0.4432	Tier II
35	NC_000012.12	12	96635989	+	CFAP54	NNNCAGCCACCCTCCCCTCC	NGG	2	0.3673	Tier II
36	NC_000014.9	14	74258410	-	VSX2	NNNGCGCCGCCCTTCCCTCC	NGG	2	0.3667	Tier II
37	NC_000002.12	2	71513210	+	DYSF	NNNCCTCCTCCCTCCCCTCC	NGG	2	0.3556	Tier II
38	NC_000016.10	16	69106345	-	HAS3	NNNGCGCGGCCCTCCCCTCC	NGG	2	0.3214	Tier II
39	NC_000007.14	7	142866419	+	EPHB6	NNNCTGCCGCCCTCCCCTCA	NGG	2	0.3182	Tier II
40	NC_000004.12	4	139735474	-	MGST2	NNNCCGCCACCCTCCCCCCC	NGG	2	0.2755	Tier II
41	NC_000004.12	4	139735474	-	MAML3	NNNCCGCCACCCTCCCCCCC	NGG	2	0.2755	Tier II
42	NC_000017.11	17	29077259	+	MYO18A	NNNCCGCTGCCCTCCTCTCC	NGG	2	0.2692	Tier II
43	NC_000005.10	5	1129486	+	SLC12A7	NNNCAGGCGCCCTCCCCTCC	NGG	2	0.2689	Tier II
44	NC_000011.10	11	646370	+	DEAF1	NNNCCACCGCCCGCCCCTCC	NGG	2	0.2609	Tier II
45	NC_000011.10	11	19601398	+	NAV2	NNNCCTCCGGCCTCCCCTCC	NGG	2	0.2593	Tier II
46	NC_000016.10	16	81095894	+	GCSH	NNNCCGCAGGCCTCCCCTCC	NGG	2	0.2528	Tier II
47	NC_000019.10	19	45954325	+	NOVA2	NNNCCGCCCCCCTCCCCTTC	NGG	2	0.2485	Tier II
48	NC_000017.11	17	44218873	+	UBTF	NNNCCGCCGCTCCCCCCTCC	NGG	2	0.2429	Tier II
49	NC_000014.9	14	103335295	-	EIF5	NNNCCGCCGCCCACCCCGCC	NGG	2	0.2308	Tier II
50	NC_000001.11	1	156053497	+	UBQLN4	NNNCCCCCGCCCTCCTCTCC	NGG	2	0.2098	Tier II
51	NC_000017.11	17	4545395	+	MYBBP1A	NNNCCCCCGCCCTCCTCTCC	NGG	2	0.2098	Tier II
52	NC_000018.10	18	907330	-	ADCYAP1	NNNCCGCCCCCCTCCCCGCC	NGG	2	0.1795	Tier II
53	NC_000017.11	17	52115674	+	CA10	NNNCCGCCCCCCTCCCCTCT	NGG	2	0.1615	Tier II
54	NC_000002.12	2	25252327	-	DNMT3A	NNNCCGCCCCCCTCCCCTCC	NAG	2	0.1396	Tier II
55	NC_000009.12	9	88388888	-	SPIN1	NNNCCGCCGGCCTCCCCGCC	NGG	2	0.1296	Tier II
56	NC_000017.11	17	74923126	-	USH1G	NNNCCGCCGCCCTCCCCTGC	NGG	1	0.125	Tier II
57	NC_000016.10	16	74576126	-	GLG1	NNNCCGCCGCCTTCACCTCC	NGG	2	0.1197	Tier II
58	NC_000020.11	20	46189111	-	CDH22	NNNCCGCCGCCCGCCCCCCC	NGG	2	0.1118	Tier II
59	NC_000001.11	1	23168722	+	LUZP1	NNNCCGCCGCCCTCCCCTCC	NCG	1	0.1071	Tier II
60	NC_000001.11	1	114902101	-	SYCP1	NNNCCGCCGCCCTCCCCTCC	NCG	1	0.1071	Tier II
61	NC_000017.11	17	81703652	-	MRPL12	NNNCCGCCGCCCTCCTCGCC	NGG	2	0.1026	Tier II
62	NC_000012.12	12	69739432	-	RAB3IP	NNNCCGCGGCCCTCCGCTCC	NGG	2	0.0989	Tier II
63	NC_000004.12	4	8857319	-	HMX1	NNNCCGCCGCCCGACCCTCC	NGG	2	0.0913	Tier II
64	NC_000011.10	11	498731	+	RNH1	NNNCCGCCGCCCGACCCTCC	NGG	2	0.0913	Tier II
65	NC_000011.10	11	117144722	-	PAFAH1B2	NNNCCGCCGCGCTACCCTCC	NGG	2	0.0875	Tier II
66	NC_000009.12	9	131641348	+	RAPGEF1	NNNTCGCCGCCCTCCCCTCC	NCG	2	0.0857	Tier II
67	NC_000003.12	3	44580167	-	ZKSCAN7	NNNCCGCCGCTCTCCCCTCC	NAG	2	0.0798	Tier II
68	NC_000006.12	6	37354721	-	RNF8	NNNCCCCCGCCCTCCCCTCC	NCG	2	0.0731	Tier II
69	NC_000019.10	19	5263057	-	PTPRS	NNNCCCCCGCCCTCCCCTCC	NCG	2	0.0731	Tier II
70	NC_000002.12	2	60552320	-	BCL11A	NNNCCGCTGCCCTCCCCTCC	NGA	2	0.0608	Tier II
71	NC_000013.11	13	107686069	-	NALF1	NNNCCGCCGTCCTCCCCTCG	NGG	2	0.0554	Tier II
72	NC_000004.12	4	2436831	+	CFAP99	NNNCCGCCGCCCTTTCCTCC	NGG	2	0.0489	Tier II
73	NC_000001.11	1	164788757	+	PBX1	NNNCCGCCGCCCTCCCCCCC	NCG	2	0.0459	Tier II
74	NC_000010.11	10	84139757	-	GHITM	NNNCCGCCGCCCTCTCCACC	NGG	2	0.0444	Tier II
75	NC_000001.11	1	27634855	+	FGR	NNNCCTCCGCCCTCTCCTCC	NGG	2	0.0444	Tier II
76	NC_000001.11	1	32200648	-	CCDC28B	NNNCCTCCGCCCTCTCCTCC	NGG	2	0.0444	Tier II
77	NC_000019.10	19	30528691	-	ZNF536	NNNCCTCCGCCCTCTCCTCC	NGG	2	0.0444	Tier II
78	NC_000020.11	20	61463621	-	CDH4	NNNCCTCCGCCCTCTCCTCC	NGG	2	0.0444	Tier II
79	NC_000004.12	4	41360739	-	LIMCH1	NNNCCGCCGGCCTCCCCTCC	NCG	2	0.0417	Tier II
80	NC_000007.14	7	100720871	+	EPO	NNNCAGCCGCCCTCTCCTCC	NGG	2	0.0381	Tier II
81	NC_000021.9	21	34792088	-	RUNX1	NNNCCGCCGCCTTCGCCTCC	NGG	2	0.0269	Tier II
82	NC_000001.11	1	10622623	+	PEX14	NNNCCGCCTCCCTCGCCTCC	NGG	2	0.0267	Tier II
83	NC_000016.10	16	47492635	-	PHKB	NNNCCGCCACCCTCCCCTCC	NTG	2	0.025	Tier II
84	NC_000016.10	16	28320011	-	SBK1	NNNCCGCCGCCCTCCCCTCC	NGC	1	0.0222	Tier II
85	NC_000006.12	6	17281455	+	RBM24	NNNCCGCCGCCCTCGCCCCC	NGG	2	0.0214	Tier II
86	NC_000016.10	16	680701	-	STUB1	NNNCCGCCGCCCTCCCCGCG	NGG	2	0.0196	Tier II
87	NC_000005.10	5	179577588	-	RUFY1	NNNCCCCCGCCCTCCCCTCC	NGC	2	0.0152	Tier II
88	NC_000005.10	5	179577640	-	RUFY1	NNNCCCCCGCCCTCCCCTCC	NGC	2	0.0152	Tier II
89	NC_000012.12	12	77743	-	IQSEC3	NNNCCCCCGCCCTCCCCTCC	NGC	2	0.0152	Tier II
90	NC_000001.11	1	27557276	+	AHDC1	NNNCCGCCCCCCTCCCCTCC	NGC	2	0.012	Tier II
91	NC_000008.11	8	131936493	+	EFR3A	NNNCCGCCCCCCTCCCCTCC	NGC	2	0.012	Tier II
92	NC_000009.12	9	127711969	-	PTRH1	NNNCCGCCGCCCGCCCCTCC	NTG	2	0.0102	Tier II
93	NC_000009.12	9	127711969	-	CFAP157	NNNCCGCCGCCCGCCCCTCC	NTG	2	0.0102	Tier II
94	NC_000013.11	13	101916710	-	FGF14	NNNCCGCCGCCTTCCCCTCC	NGT	2	0.0087	Tier II
95	NC_000016.10	16	81923441	+	PLCG2	NNNCCGCCTCCCTCCCCTCC	NGT	2	0.0086	Tier II
96	NC_000002.12	2	12718803	-	TRIB2	NNNCCGCCGCCCTCTCCTCC	NCG	2	0.0071	Tier II
97	NC_000016.10	16	85625203	+	GSE1	NNNCCGCCGCCCTCCTCTCC	NGC	2	0.0068	Tier II
98	NC_000012.12	12	116878950	+	HRK	NNNCCGCCGCCCTCCCCTGC	NGC	2	0.0028	Tier II
99	NC_000008.11	8	144632050	+	ARHGAP39	NNNCCACCGCCCTGCCCTCC	NGG	2	0.0	Tier II
100	NC_000009.12	9	131546635	+	PRRT1B	NNNCCGCCGCCCCGCCCTCC	NGG	2	0.0	Tier II
101	NC_000002.12	2	104857188	+	POU3F3	NNNCCGCCGCCCTCCCCTCC	NCC	2	0.0	Tier II
102	NC_000010.11	10	13347739	+	SEPHS1	NNNCCGCCGCCCTCCCCTCC	NCT	2	0.0	Tier II
103	NC_000003.12	3	153162362	+	RAP2B	NNNCCGCCTCCCTGCCCTCC	NGG	2	0.0	Tier II
104	NC_000019.10	19	31344729	-	TSHZ3-AS1	NNNCCGCCGCCCTCCACTCC	NGG	1	1.0	Tier III
105	NC_000016.10	16	2955337	+	LOC105371055	NNNACACCGCCCTCCCCTCC	NGG	2	0.8421	Tier III
106	NC_000017.11	17	77433787	+	LOC112268199	NNNCCCCTGCCCTCCCCTCC	NGG	2	0.5966	Tier III
107	NC_000001.11	1	16708155	+	ESPNP	NNNCCGCCCCCCTCCCCTCC	NGG	1	0.5385	Tier III
108	NC_000003.12	3	11062670	+	LOC101927467	NNNCCGCTGCCCTCCCCTCA	NGG	2	0.4375	Tier III
109	NC_000008.11	8	142475569	-	LOC124902075	NNNCCGCCGCCCTCCACGCC	NGG	2	0.3333	Tier III
110	NC_000012.12	12	2959107	-	LOC124902859	NNNCCCGCGCCCTCCCCTCC	NGG	2	0.3209	Tier III
111	NC_000003.12	3	127547885	+	LINC02034	NNNCCGCCCCCCTCCCCTTC	NGG	2	0.2485	Tier III
112	NC_000017.11	17	44218873	+	ATXN7L3-AS1	NNNCCGCCGCTCCCCCCTCC	NGG	2	0.2429	Tier III
113	NC_000007.14	7	438369	+	LOC116435278	NNNCCGCCGCCCTCCCTCCC	NGG	2	0.2	Tier III
114	NC_000009.12	9	88388888	-	LOC124902204	NNNCCGCCGGCCTCCCCGCC	NGG	2	0.1296	Tier III
115	NC_000011.10	11	134037396	-	LINC02731	NNNCCACCGCCCTCCCCTCC	NCG	2	0.1071	Tier III
116	NC_000001.11	1	114902101	-	LOC105378915	NNNCCGCCGCCCTCCCCTCC	NCG	1	0.1071	Tier III
117	NC_000003.12	3	44580167	-	MPRIPP1	NNNCCGCCGCTCTCCCCTCC	NAG	2	0.0798	Tier III
118	NC_000003.12	3	44580167	-	ZKSCAN7-AS1	NNNCCGCCGCTCTCCCCTCC	NAG	2	0.0798	Tier III
119	NC_000019.10	19	12141225	-	ZNF625-ZNF20	NNNCAGCCGCCCTCCCCTGC	NGG	2	0.0714	Tier III
120	NC_000023.11	X	2584476	-	LINC03112	NNNCCGCCGCCCCCTCCTCC	NGG	2	0.0526	Tier III
121	NC_000024.10	Y	2584476	-	LINC03112	NNNCCGCCGCCCCCTCCTCC	NGG	2	0.0526	Tier III
122	NC_000019.10	19	16628530	-	LOC105372296	NNNCCGCCGCCCTCCCCGCC	NCG	2	0.0357	Tier III
123	NC_000019.10	19	1940868	-	LOC105372238	NNNCCGCCGCCCGCCCCTCC	NCG	2	0.028	Tier III
124	NC_000005.10	5	179577588	-	LOC128966623	NNNCCCCCGCCCTCCCCTCC	NGC	2	0.0152	Tier III
125	NC_000005.10	5	179577640	-	LOC128966623	NNNCCCCCGCCCTCCCCTCC	NGC	2	0.0152	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000070.6	4	155760923	+	Atad3a	NNNCCGCCGCCCTCCGCCCC	NGG	2	0.0659	Tier I
2	NC_000073.6	7	96644069	+	Tenm4	NNNCCGCCCCCCTCCCCTCC	NGC	2	0.012	Tier I
3	NC_000073.6	7	28268170	-	Eid2	NNNCCGCCGCCCTGCCTTCC	NGG	2	0.0	Tier I
4	NC_000071.6	5	129942361	+	Vkorc1l1	NNNCCGCCGCCCTGGCCTCC	NGG	2	0.0	Tier I
5	NC_000068.7	2	44739216	+	Gtdc1	NNNTCGCTGCCCTCCCCTCC	NGG	2	0.7	Tier II
6	NC_000081.6	15	102257245	-	Rarg	NNNCCCCCGCCCTCCCCTCC	NGG	1	0.6818	Tier II
7	NC_000084.6	18	67775154	-	Seh1l	NNNCCGCCTCCCTCCCCTCC	NGG	1	0.5333	Tier II
8	NC_000080.6	14	62759981	-	Ints6	NNNCCACCGCCGTCCCCTCC	NGG	2	0.4444	Tier II
9	NC_000067.6	1	168326055	+	Pbx1	NNNCCCCAGCCCTCCCCTCC	NGG	2	0.4432	Tier II
10	NC_000072.6	6	97395960	-	Frmd4b	NNNCCTCAGCCCTCCCCTCC	NGG	2	0.4333	Tier II
11	NC_000077.6	11	72465132	-	Spns2	NNNCCGCCACCCTCCCCACC	NGG	2	0.4286	Tier II
12	NC_000072.6	6	125096470	+	Chd4	NNNCCGCCCCCCCCCCCTCC	NGG	2	0.4251	Tier II
13	NC_000081.6	15	80454444	-	Enthd1	NNNCCGCCCCCCCCCCCTCC	NGG	2	0.4251	Tier II
14	NC_000078.6	12	15814937	+	Trib2	NNNTCGCCGCCCTCCCTTCC	NGG	2	0.3733	Tier II
15	NC_000068.7	2	145963070	+	Cfap61	NNNCCCCCCCCCTCCCCTCC	NGG	2	0.3671	Tier II
16	NC_000085.6	19	16132690	+	Gnaq	NNNCCGCCGCCGCCCCCTCC	NGG	2	0.3509	Tier II
17	NC_000081.6	15	74733651	-	Lypd2	NNNCCGCAGCCCTCCCCTCA	NGG	2	0.325	Tier II
18	NC_000069.6	3	142881566	+	Pkn2	NNNCGGCCGCCTTCCCCTCC	NGG	2	0.3231	Tier II
19	NC_000071.6	5	73213950	-	Fryl	NNNCCGGCACCCTCCCCTCC	NGG	2	0.3025	Tier II
20	NC_000072.6	6	113195066	-	Lhfpl4	NNNCCACCGCCCGCCCCTCC	NGG	2	0.2609	Tier II
21	NC_000071.6	5	144255318	+	Bri3	NNNCCGCCGCCATCCCCGCC	NGG	2	0.2381	Tier II
22	NC_000084.6	18	6765158	-	Rab18	NNNCCGCCTCCCTCCCCTAC	NGG	2	0.2286	Tier II
23	NC_000079.6	13	58402925	-	Hnrnpk	NNNCCCCCGCCCTCCCCGCC	NGG	2	0.2273	Tier II
24	NC_000079.6	13	58402925	-	Rmi1	NNNCCCCCGCCCTCCCCGCC	NGG	2	0.2273	Tier II
25	NC_000070.6	4	152274180	-	Gpr153	NNNCCGCCGCCCTCCCTCCC	NGG	2	0.2	Tier II
26	NC_000073.6	7	141061056	-	B4galnt4	NNNCCCCCGCCCGCCCCTCC	NGG	2	0.1779	Tier II
27	NC_000082.6	16	94570253	+	Dyrk1a	NNNCCGCCGCCCTCCGCACC	NGG	2	0.1026	Tier II
28	NC_000068.7	2	151039209	-	Nanp	NNNCCGTCGCCCTCCCCTGC	NGG	2	0.1016	Tier II
29	NC_000069.6	3	137865000	-	H2az1	NNNCCGCCGCCCGCCCCGCC	NGG	2	0.087	Tier II
30	NC_000077.6	11	35925717	-	Wwc1	NNNCCTCCGCCCTCCCCTCC	NCG	2	0.0714	Tier II
31	NC_000071.6	5	88886961	+	Slc4a4	NNNCCGCCGCCGTCCCCTCC	NCG	2	0.0476	Tier II
32	NC_000067.6	1	91053047	-	Lrrfip1	NNNCCGCCTCCCTCCCCTCC	NGA	2	0.037	Tier II
33	NC_000073.6	7	144838953	-	Fgf3	NNNCCGCCACCCTCCCCTCC	NTG	2	0.025	Tier II
34	NC_000080.6	14	52014274	+	Zfp219	NNNCCGCGGCCCTCCCCTCC	NTG	2	0.025	Tier II
35	NC_000083.6	17	29888062	-	Mdga1	NNNCCGGCGCCCTCGCCTCC	NGG	2	0.0235	Tier II
36	NC_000071.6	5	119796765	+	Tbx5	NNNCCGCTGCCCTCCCCTCC	NGC	2	0.0194	Tier II
37	NC_000086.7	X	12079413	+	Bcor	NNNCCGCCGCCCTCCCCTTC	NTG	2	0.018	Tier II
38	NC_000073.6	7	54835597	+	Luzp2	NNNCCACCGCCCTCCCCTCC	NGT	2	0.0161	Tier II
39	NC_000068.7	2	27396682	+	Vav2	NNNCAGCCGCCCTCCCCTCC	NGC	2	0.0127	Tier II
40	NC_000075.6	9	44708536	-	Phldb1	NNNCCGCCTCCCTCCCCTCC	NGC	2	0.0119	Tier II
41	NC_000071.6	5	136252710	-	Cux1	NNNCTGCCGCCCTCCCCTCC	NGT	2	0.0103	Tier II
42	NC_000077.6	11	58323178	+	Zfp672	NNNCCGCCGCCCTCCGGTCC	NGG	2	0.009	Tier II
43	NC_000068.7	2	157914708	-	Vstm2l	NNNCCGCCGCCCTCTCCTGC	NGG	2	0.0083	Tier II
44	NC_000078.6	12	110978998	-	Ankrd9	NNNCCGCCGCCCTCCCCTCG	NCG	2	0.0063	Tier II
45	NC_000075.6	9	109095478	-	Plxnb1	NNNCCGCCGCCCGCCCCTCC	NGC	2	0.0058	Tier II
46	NC_000081.6	15	84923677	-	Nup50	NNNCCGCCGCCCCGCCCTCC	NGG	2	0.0	Tier II
47	NC_000070.6	4	152027402	-	Zbtb48	NNNCCGCCGCCCTGCCCTAC	NGG	2	0.0	Tier II
48	NC_000067.6	1	13660524	-	Lactb2	NNNCCGCCGCCCTGCCCTCC	NAG	2	0.0	Tier II
49	NC_000070.6	4	151108786	+	Camta1	NNNCCGCCGCCCTGCCCTCG	NGG	2	0.0	Tier II
50	NC_000068.7	2	28514116	-	Ralgds	NNNCCTCCGCCCTGCCCTCC	NGG	2	0.0	Tier II
51	NC_000070.6	4	150033324	+	Gm13067	NNNCCCCCGCCCTCCCCTCC	NGG	1	0.6818	Tier III
52	NC_000084.6	18	67775154	-	Gm26910	NNNCCGCCTCCCTCCCCTCC	NGG	1	0.5333	Tier III
53	NC_000083.6	17	15899273	-	Gm41533	NNNCCCCAGCCCTCCCCTCC	NGG	2	0.4432	Tier III
54	NC_000067.6	1	74789258	+	Gm32099	NNNCCCCCGCCCTCCCTTCC	NGG	2	0.3182	Tier III
55	NC_000071.6	5	144255318	+	2900089D17Rik	NNNCCGCCGCCATCCCCGCC	NGG	2	0.2381	Tier III
56	NC_000071.6	5	119668275	-	Tbx3os1	NNNCCGCCGCGCCCCCCTCC	NGG	2	0.1974	Tier III
57	NC_000067.6	1	21272354	-	Gm7094	NNNCAGCCGCTCTCCCCTCC	NGG	2	0.1758	Tier III
58	NC_000081.6	15	38990315	-	Gm4740	NNNCCGCCGCCCTCCGCCCC	NGG	2	0.0659	Tier III
59	NC_000074.6	8	125899030	-	A730098A19Rik	NNNCCGCCCCCCTCTCCTCC	NGG	2	0.0359	Tier III
60	NC_000078.6	12	24383183	+	Gm9315	NNNCCGCCGCCCTACCCTCC	NGC	2	0.0078	Tier III
61	NC_000075.6	9	109095478	-	Gm7628	NNNCCGCCGCCCGCCCCTCC	NGC	2	0.0058	Tier III

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Other clones with same target sequence:

(none)