Construct: ORF TRCN0000465313
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003939.1_s317c1
- Derived from:
- ccsbBroadEn_12639
- DNA Barcode:
- GCCTACCTGTGCTGAACTGAAAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- THSD4 (79875)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465313
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79875 | THSD4 | thrombospondin type 1 domai... | NM_001286429.2 | 73.6% | 73.2% | (many diffs) |
| 2 | human | 79875 | THSD4 | thrombospondin type 1 domai... | XM_017022586.1 | 72.6% | 72.2% | (many diffs) |
| 3 | human | 79875 | THSD4 | thrombospondin type 1 domai... | XM_017022585.1 | 71.8% | 71.5% | (many diffs) |
| 4 | human | 79875 | THSD4 | thrombospondin type 1 domai... | XM_017022584.1 | 71.6% | 71.3% | (many diffs) |
| 5 | human | 79875 | THSD4 | thrombospondin type 1 domai... | XM_011522044.2 | 71% | 70.6% | (many diffs) |
| 6 | human | 79875 | THSD4 | thrombospondin type 1 domai... | XM_017022583.1 | 70% | 69.6% | (many diffs) |
| 7 | human | 79875 | THSD4 | thrombospondin type 1 domai... | XM_011522043.3 | 69.6% | 69.2% | (many diffs) |
| 8 | human | 79875 | THSD4 | thrombospondin type 1 domai... | XM_017022582.2 | 66.9% | 66.5% | (many diffs) |
| 9 | human | 79875 | THSD4 | thrombospondin type 1 domai... | NM_024817.2 | 47.6% | 47.3% | (many diffs) |
| 10 | human | 79875 | THSD4 | thrombospondin type 1 domai... | XM_006720692.3 | 47.6% | 47.3% | (many diffs) |
| 11 | mouse | 207596 | Thsd4 | thrombospondin, type I, dom... | NM_172444.4 | 64.9% | 68.2% | (many diffs) |
| 12 | mouse | 207596 | Thsd4 | thrombospondin, type I, dom... | NM_001040426.3 | 42% | 44.1% | (many diffs) |
| 13 | mouse | 207596 | Thsd4 | thrombospondin, type I, dom... | XM_006510906.3 | 42% | 44.1% | (many diffs) |
| 14 | mouse | 207596 | Thsd4 | thrombospondin, type I, dom... | XM_006510907.3 | 42% | 44.1% | (many diffs) |
| 15 | mouse | 207596 | Thsd4 | thrombospondin, type I, dom... | XM_006510905.3 | 41.8% | 43.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1536
- ORF length:
- 1470
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta caagagcaac aactatttgg ccctgagaag tcgttctgga cgctccatca 121 tcaatgggaa ctgggcaatt gatcgaccag gaaaatacga gggcggaggg accatgttca 181 cctacaagcg tccaaatgag atttcgagca ctgccggaga gtcctttttg gcggaaggtc 241 ccaccaacga gatcttggat gtctacatga tacaccagca gccaaaccca ggcgtgcact 301 acgagtacgt gatcatgggg accaacgcca tcagccccca ggtgccaccc cacaggagac 361 caggggaacc cttcaatggc cagatggtga cagaaggcag gagccaggag gagggagaac 421 agaaagggag gaacgaggag aaggaagact tgcgtgggga ggcccctgag atgttcacct 481 cagaatcggc acagaccttc ccagtcaggc atccagacag attttctccc catcgaccgg 541 acaacttggt gccaccagca ccgcagcccc cacggcgcag ccgggatcac aactggaagc 601 agcttgggac aacagaatgt tccacgacct gtgggaaagg atcgcagtac cctattttcc 661 gctgtgtgca cagaagcact catgaagagg ctcctgagag ttactgtgac tccagcatga 721 agccgacccc cgaggaggag ccctgcaaca tcttcccttg cccagccttc tgggacatcg 781 gggagtggtc tgagtgcagc aagacctgtg gcctgggcat gcagcaccgc caggttctgt 841 gccgccaggt gtacgccaac cgcagcctga cggtgcagcc ctaccgctgc cagcacctgg 901 agaaacctga gaccaccagc acctgccaac tcaagatctg cagcgagtgg cagatccgga 961 ccgactggac ctcgtgctcg gtgccctgcg gcgtgggaca gaggacccgt gatgtgaagt 1021 gtgtgagcaa cattggggat gtggttgacg atgaggaatg caacatgaag ctccggccga 1081 atgacattga gaactgcgac atgggaccct gtgccaagag ctggttcctc accgagtgga 1141 gcgaaaggtg ctcagcggag tgtggggccg gagtgcggac acgctcggtg GTGTGCATGA 1201 CCAACCATGT CAGCAGCCTG CCCCTGGAGG GCTGTGGGAA CAACCGGCCG GCAGAGGCCA 1261 CCCCATGTGA CAACGGACCC TGCACGGGCA AGGTGGAGTG GTTTGCCGGG AGCTGGAGTC 1321 AGTGTTCCAT CGAGTGTGGG AGCGGGACGC AACAGAGGGA GGTGATTTGT GTTAGAAAGA 1381 ATGCAGACAC CTTTGAAGTG TTGGACCCCT CTGAATGTTC TTTCCTGGAG AAACCCCCCA 1441 GCCAGCAATC CTGCCACCTC AAGCCTTGCG GAGCCAAATG GTTTAGCACC GAATGGAGCA 1501 TGAGTCTACA AAGAGCCATG CTCACCAGGC CAAGTTGCCC AACTTTCTTG TACAAAGTGG 1561 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1621 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1681 AGCCTACCTG TGCTGAACTG AAAGGACGCG TTAAGTCgac aatcaacctc tggattacaa 1741 aatttgtgaa agatt