Construct: ORF TRCN0000465457
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014163.1_s317c1
- Derived from:
- ccsbBroadEn_14337
- DNA Barcode:
- ACTCAATCTGAGCTGACAATACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- L3MBTL4 (91133)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465457
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | NM_001365767.2 | 99.9% | 99.8% | 1597T>C |
| 2 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | XM_011525763.2 | 93.4% | 89.4% | (many diffs) |
| 3 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | NM_001365766.2 | 92% | 90.8% | (many diffs) |
| 4 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | XM_006722364.2 | 90.6% | 90.2% | (many diffs) |
| 5 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | XM_011525762.2 | 89.8% | 84.6% | (many diffs) |
| 6 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | NM_001365768.1 | 89.7% | 87.4% | (many diffs) |
| 7 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | NM_001365769.1 | 89.7% | 87.4% | (many diffs) |
| 8 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | XM_017026074.1 | 86.3% | 79% | (many diffs) |
| 9 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | NM_001330559.1 | 83.6% | 80.1% | (many diffs) |
| 10 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | NM_001365765.1 | 83.6% | 80.1% | (many diffs) |
| 11 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | NM_001365770.1 | 81.3% | 77.5% | (many diffs) |
| 12 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | NM_173464.3 | 81.3% | 77.5% | (many diffs) |
| 13 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | XM_011525760.1 | 80.7% | 74.1% | (many diffs) |
| 14 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | XM_011525757.1 | 77.8% | 74.2% | (many diffs) |
| 15 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | XM_011525758.1 | 77.8% | 74.2% | (many diffs) |
| 16 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | XM_011525759.1 | 77.8% | 74.2% | (many diffs) |
| 17 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | XR_935072.1 | 70.9% | (many diffs) | |
| 18 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | XM_017026077.1 | 69.1% | 68.3% | (many diffs) |
| 19 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | NR_158609.1 | 43.8% | (many diffs) | |
| 20 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | NR_158610.1 | 41.2% | (many diffs) | |
| 21 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | XM_011525767.2 | 34% | 30.2% | (many diffs) |
| 22 | human | 91133 | L3MBTL4 | L3MBTL histone methyl-lysin... | NR_158611.1 | 25.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1668
- ORF length:
- 1602
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa acagcccaac aggaaaagga agcttaatat ggattccaaa gagcgtttgg 121 atcaggacgg acgcttggag caagctgaag aggaaaagaa gcccaaggat agcacaaccc 181 ctttgagtca cgtcccttca gcggctgcac agggagcatg gtcttgggag tggtacttga 241 aagaacagaa ggctgtcgca gcacctgttg agctgttttc caaggatcag tcctttccag 301 agcatgaaaa tggttttcag attggaatga gattagaagg cattgatccc cgacatccat 361 cggtattctg tgtgctttct gtagcggagg tttgtggtta ccgtctaaga cttcattttg 421 atggttattt aagttgctat gatttttgga ccaatgctgg ttcccctgac attcatccag 481 taggatggtg tgaaaagacc aaacatgaac tgcacatccc taagggttat agaaaagata 541 aatttgtttg gatggattac ttgaaggcct gcaaattgca aaatgctcca aagaaattat 601 tcagaaacag aagtcctaat gggccaatgt ctaaagaatt tcaggttgga atgaagctgg 661 aggccgtgga caggaagaac ccttccttgg tgtgtgtggc gaccatagca gatattgttg 721 aagatcgctt actagtgcat tttgacaact gggatgatag ttacgattac tggtgcgatg 781 ttaatagccc ttatgtccag ccagttggtt ggtgtcagga gaatggaaga actctgatag 841 caccccaagg ttatcccaat ccagaaaatt tttcctggac agaatacctg gaagctactc 901 aaaccaatgc agttcctgcc aaagttttta aaatgaggtt gcctcatggt tttctgccaa 961 atatgaaact tgaagttgtg gataaacgga accccaggtt aattcgtgtt gctacgattg 1021 tagatgttga tgaccaaaga gtaaaggttc attttgatgg ttgggaccat aagtatgact 1081 actgggtgga ggcagacagc cctgatatcc acccgatcgg atggtgtgat gtcacagggc 1141 atccactgga agtgccacag cgaacgaatg acctgaagat ccttccaggt caagctgtct 1201 gtcctactcc cgggtgccga ggaataggcc atatccgtgg tccacgttat tcgggacatc 1261 acagtgcttt tggctgcccg tattcagaca tgaacttgaa aaaggaggca acacttcacg 1321 atcgtttgag agaacaaaca caggcaaatt tggaatcaga ctcttcacat tcaaaatcaa 1381 aaagcctctg tagtttgaac ttcaatgGAA AACATGAAAA GGTGAACAGT CAGCCCAGAC 1441 TTGTACAGCA GGCAAAGTGT TTGAAAATCA AAGGAAAAGA AGATATTGAC TTGGATAATC 1501 TCTTCAGAGG CCGAGTGCGG TGGCTCATGC CTGTAATCCC AGCACTTTGG GAGGCCGAGG 1561 CAGGCAGATC ACGAGATCAG GAGATCAAGA CCATCCTGGC CAACGCAGTG AAACCCCGTC 1621 TCTACCAAAA ATACAAAAAA TTAGCCAGGC GTGGTGGCGG GCGCCTATAC CCAACTTTCT 1681 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1741 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1801 GTGGAAAGGA CGAACTCAAT CTGAGCTGAC AATACGAACG CGTTAAGTCg acaatcaacc 1861 tctggattac aaaatttgtg aaagatt