Construct: ORF TRCN0000465689
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003307.2_s317c1
- Derived from:
- BRDN0000391630
- DNA Barcode:
- TGACCGAAAAAAAATTTTTCGTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAP2K2 (5605)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465689
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5605 | MAP2K2 | mitogen-activated protein k... | NM_030662.3 | 99.5% | 68.1% | (many diffs) |
| 2 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_006722799.2 | 76.5% | 71.2% | 453C>T;660C>A;705_706ins280 |
| 3 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026989.1 | 75% | 53.3% | (many diffs) |
| 4 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026990.1 | 70.6% | 58.9% | (many diffs) |
| 5 | human | 407835 | LOC407835 | mitogen-activated protein k... | NR_002144.1 | 65.7% | (many diffs) | |
| 6 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026991.1 | 61.3% | 77.4% | (many diffs) |
| 7 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | NM_001347144.1 | 86.9% | 66.4% | (many diffs) |
| 8 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | NM_023138.5 | 86.7% | 65.7% | (many diffs) |
| 9 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | XM_006513684.3 | 44.9% | 20.9% | (many diffs) |
| 10 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | XM_006513683.3 | 44.8% | 20.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 999
- ORF length:
- 933
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ggcccggagg aagccggtgc tgccggcgct caccatcaac cctaccatcg 121 ccgagggccc atcccctacc agcgagggcg cctccgaggc aaacctggtg gacctgcaga 181 agaagctgga ggagctggaa cttgacgagc agcagaagaa gcggctggaa gcctttctca 241 cccagaaagc caaggtcggc gaactcaaag acgatgactt cgaaaggatc tcagagctgg 301 gcgcgggcaa cggcggggtg gtcaccaaag tccagcacag accctcgggc ctcatcatgg 361 ccaggaagct gatccacctt gagatcaagc cggccatccg gaaccagatc atccgcgagc 421 tgcaggtcct gcacgaatgc aactcgccgt acatcgtggg cttctacggg gccttctaca 481 gtgacgggga gatcagcatt tgcatggaac acatggatgg cggctccctg gaccaggtgc 541 tgaaagaggc caagaggatt cccgaggaga tcctggggaa agtcagcatc gcggttctcc 601 ggggcttggc gtacctccga gagaagcacc agatcatgca ccgagatgtg aagccctcca 661 acatcctcgt gaactctaga ggggagatca agctgtgtga cttcggggtg agcggccagc 721 tcatagactc catggccaac tccttcgtgg gcacgcgctc ctacatggct ccggagcggt 781 tgcagggcac acattactcg gtgcagtcgg acatctggag catgggcctg tccctggtgg 841 agctggcccg gaggaaggta ccccatcccc ccgcccgacg ccaaagagct ggaggccatc 901 tttggccggc ccgtggtcga cggggaagaa ggagagcctc acagcatctc gcctcggccg 961 aggccccccg ggcgccccgt cagcggtcac gggatggata gccggcctgc catggccatc 1021 tttgaactcc tggactatat tgtgaacgag ccacctccTA AGCTGCCCAA CGGTGTGTTC 1081 ACCCCCGACT TCCAGGAGTT TGTCAATAAA TGCCTCATCA AGAACCCAGC GGAGCGGGCG 1141 GACCTGAAGA TGCTCACAAA CCACACCTTC ATCAAGCGGT CCGAGGTGGA AGAAGTGGAT 1201 TTTGCCGGCT GGTTGTGTAA AACCCTGCGG CTGAACCAGC CCGGCACACC CACGCGCACC 1261 GCCGTGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT 1321 CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT 1381 TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATGACCGAA AAAAAATTTT TCGTTTACGC 1441 GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt