Construct: ORF TRCN0000465718
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000198.1_s317c1
- Derived from:
- ccsbBroadEn_11277
- DNA Barcode:
- TCGTTCTATACCCGCCGCTGGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HAT1 (8520)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465718
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8520 | HAT1 | histone acetyltransferase 1 | XM_006712808.3 | 80.7% | 80.6% | 1_237del;923T>C |
| 2 | human | 8520 | HAT1 | histone acetyltransferase 1 | XM_017005136.2 | 80.7% | 80.6% | 1_237del;923T>C |
| 3 | human | 8520 | HAT1 | histone acetyltransferase 1 | XM_017005137.2 | 80.7% | 80.6% | 1_237del;923T>C |
| 4 | human | 8520 | HAT1 | histone acetyltransferase 1 | XM_024453186.1 | 80.7% | 80.6% | 1_237del;923T>C |
| 5 | human | 8520 | HAT1 | histone acetyltransferase 1 | XM_024453187.1 | 80.7% | 80.6% | 1_237del;923T>C |
| 6 | human | 8520 | HAT1 | histone acetyltransferase 1 | NM_003642.4 | 79.6% | 79.4% | 1_255del;941T>C |
| 7 | human | 8520 | HAT1 | histone acetyltransferase 1 | NR_027862.1 | 62.2% | 1_255del;1258_1609del | |
| 8 | mouse | 107435 | Hat1 | histone aminotransferase 1 | NM_026115.4 | 73.4% | 74.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1068
- ORF length:
- 1002
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ccgtgttgaa tatgcatcta aagttgatga gaactttgac tgtgtagagg 121 cagatgatgt tgagggcaaa attagacaaa tcattccacc tggattttgc acaaacacga 181 atgatttcct ttctttactg gaaaaggaag ttgatttcaa gccattcgga accttacttc 241 atacctactc agttctcagt ccaacaggag gagaaaactt tacctttcag atatataagg 301 ctgacatgac atgtagaggc tttcgagaat atcatgaaag gcttcagacc tttttgatgt 361 ggtttattga aactgctagc tttattgacg tggatgatga aagatggcac tactttctag 421 tatttgagaa gtataataag gatggagcta cgctctttgc gaccgtaggc tacatgacag 481 tctataatta ctatgtgtac ccagacaaaa cccggccacg tgtaagtcag atgctgattt 541 tgactccatt tcaaggtcaa ggccatggtg ctcaacttct tgaaacagtt catagatact 601 acactgaatt tcctacagtt cttgatatta cagcggaaga tccatccaaa agctatgtga 661 aattacgaga ctttgtgctt gtgaagcttt gtcaagattt gccctgtttt tcccgggaaa 721 aattaatgca aggattcaat gaagatatgg cgatagaggc acaacagaag ttcaaaataa 781 ataagcaaca cgctagaagg gtttatgaaa ttcttcgact actggtaact gacatgagtg 841 atgccgaaca atacagaagc tacagactgg atattaaaag aagactaatt agcccatata 901 agaaaaagca gagagatctt gctaagatga gaaaatgtct cagaccagaa gaactgacaa 961 accagatgaa ccaaatagaa aTAAGCATGC AACATGAACA GCTGGAAGAG AGTTTTCAGG 1021 AACTAGTGGA AGATTACCGG CGTGTTATTG AACGACTTGC TCAAGAGTAC CCAACTTTCT 1081 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1141 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1201 GTGGAAAGGA CGATCGTTCT ATACCCGCCG CTGGTTGACG CGTTAAGTCg acaatcaacc 1261 tctggattac aaaatttgtg aaagatt