Construct: ORF TRCN0000465829
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001196.1_s317c1
- Derived from:
- ccsbBroadEn_10494
- DNA Barcode:
- CCCTACCTAGCGGGTCATGTGAGA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZSCAN12 (9753)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465829
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9753 | ZSCAN12 | zinc finger and SCAN domain... | NM_001163391.2 | 94.7% | 93.3% | (many diffs) |
| 2 | human | 9753 | ZSCAN12 | zinc finger and SCAN domain... | NM_001368124.1 | 94.7% | 93.3% | (many diffs) |
| 3 | human | 9753 | ZSCAN12 | zinc finger and SCAN domain... | XM_011515014.3 | 94.7% | 93.3% | (many diffs) |
| 4 | human | 9753 | ZSCAN12 | zinc finger and SCAN domain... | XM_011515015.2 | 94.7% | 93.3% | (many diffs) |
| 5 | human | 9753 | ZSCAN12 | zinc finger and SCAN domain... | XM_017011528.2 | 94.7% | 93.3% | (many diffs) |
| 6 | human | 9753 | ZSCAN12 | zinc finger and SCAN domain... | XM_024446598.1 | 94.7% | 93.3% | (many diffs) |
| 7 | human | 9753 | ZSCAN12 | zinc finger and SCAN domain... | XM_011515017.3 | 93.5% | 93.5% | 1695_1696ins116 |
| 8 | human | 9753 | ZSCAN12 | zinc finger and SCAN domain... | NR_028077.2 | 41.8% | 1_159del;1956delT;1972_4323del | |
| 9 | human | 9753 | ZSCAN12 | zinc finger and SCAN domain... | NR_136509.2 | 40.2% | (many diffs) | |
| 10 | human | 9753 | ZSCAN12 | zinc finger and SCAN domain... | NR_136512.2 | 39.9% | (many diffs) | |
| 11 | human | 9753 | ZSCAN12 | zinc finger and SCAN domain... | NR_136510.2 | 39.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1878
- ORF length:
- 1812
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc atctacttgg gctatccagg cccacatgga ccaggatgaa cctttggaag 121 taaagataga ggaagagaaa tataccacca gacaggattg ggacctgcgt aaaaacaaca 181 cccatagcag agaggtcttc cgtcagtact tcagacagtt ctgctaccag gagacatctg 241 gtccccgtga ggctttgagc cgactccgag aactttgcca tcagtggctg aggccagaga 301 cccacaccaa agaacagatt ctggagctgc tggtgctgga gcagttcctg accatcctac 361 ctgaggagct ccaggcctgg gtgcaggagc agcatccaga gagtggggag gaggtggtga 421 ctgtgctgga ggatttagag agagaactgg atgaaccagg agagcaggtc tcagtccaca 481 ctggggaaca ggaaatgttc ttgcaggaga cggtacgtct acgaaaagaa ggagaaccca 541 gtatgtccct ccagtccatg aaagcccagc caaagtatga atctccagaa cttgaatccc 601 aacaggagca agttttagat gttgagactg gaaatgagta tgggaattta aagcaagaag 661 tttctgaaga aatggaacca catgggaaga catccagtaa atttgaaaat gatatgtcca 721 agtctgctag gtgtggagaa actcgtgaac ctgaagaaat aacagaagag ccctctgctt 781 gctccagaga agataaacaa cctacctgtg atgaaaatgg agtaagcctg actgagaact 841 ctgaccatac tgaacatcag agaatctgtc caggagaaga atcttacgga tgtgatgact 901 gtggaaaagc ttttagtcag cactcacacc tcatagaaca tcagaggatc catactggag 961 atagacccta caaatgcgaa gaatgtggaa aagctttccg tgggagaact gtgcttattc 1021 gacacaaaat aatacacact ggagagaaac cgtataagtg taatgaatgt ggcaaagcct 1081 ttggccggtg gtcagctctt aaccaacatc agagacttca cacaggagaa aaacactatc 1141 actgtaatga ctgtggcaaa gcctttagtc agaaagcagg cctctttcac catatcaaga 1201 tccacacaag agacaaacct tatcagtgca ctcagtgtaa taaaagtttt agtcggcgtt 1261 ccatacttac tcagcatcaa ggagttcata ccggcgccaa gccgtatgag tgcaacgagt 1321 gtggaaaagc ctttgtttat aactcatccc ttgtttccca tcaggaaatc caccacaaag 1381 aaaaatgcta tcagtgtaag gaatgtggga aatccttcag tcagagtggc cttattcagc 1441 atcagagaat tcacactggg gaaaaaccct acaaatgtga cgtatgtgaa aaagccttta 1501 ttcaaaggac aagtcttaca gaacatcagc gaattcacac tggagaaaga ccctataaat 1561 gtgataagtg tgggaaggcg tttactcaaa gatcagtccT CACGGAACAT CAGAGAATCC 1621 ACACTGGAGA GAGGCCCTAC AAGTGTGATG AGTGTGGGAA TGCCTTCCGA GGAATCACCA 1681 GCCTCATTCA GCATCAGAGA ATCCACACTG GGGAGAAGCC CTACCAATGT GATGAGTGTG 1741 GAAAAGCCTT CAGACAGAGG AAGAAGACCA GCTACAAGGA AATACTTCTG AAAAATCACT 1801 CTGAACCCCA AGCTGGAGTG AATCTTCTCC TGAGCTCTCT CATTCCAGAA TGGCAATCTT 1861 GTTCAGGAGA GATCTCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 1921 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 1981 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCCTACCT AGCGGGTCAT 2041 GTGAGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt