Transcript: Human XM_011515017.3

PREDICTED: Homo sapiens zinc finger and SCAN domain containing 12 (ZSCAN12), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZSCAN12 (9753)
Length:
7317
CDS:
181..1878

Additional Resources:

NCBI RefSeq record:
XM_011515017.3
NBCI Gene record:
ZSCAN12 (9753)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011515017.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000013506 CGTTCCATACTTACTCAGCAT pLKO.1 1372 CDS 100% 2.640 2.112 N ZSCAN12 n/a
2 TRCN0000415215 GAAATGAGTATGGGAATTTAA pLKO_005 746 CDS 100% 15.000 10.500 N ZSCAN12 n/a
3 TRCN0000432343 ATCAGTGCACTCAGTGTAATA pLKO_005 1337 CDS 100% 13.200 9.240 N ZSCAN12 n/a
4 TRCN0000013503 CCTGGATGATTATCTCTGTTT pLKO.1 6178 3UTR 100% 4.950 3.465 N ZSCAN12 n/a
5 TRCN0000013504 CCAAAGTATGAATCTCCAGAA pLKO.1 685 CDS 100% 4.050 2.835 N ZSCAN12 n/a
6 TRCN0000013507 CCATCAGGAAATCCACCACAA pLKO.1 1473 CDS 100% 4.050 2.835 N ZSCAN12 n/a
7 TRCN0000013505 CCAGAGAAGATAAACAACCTA pLKO.1 899 CDS 100% 3.000 2.100 N ZSCAN12 n/a
8 TRCN0000139610 CGAACTCCTGACCTTGTGATA pLKO.1 6740 3UTR 100% 4.950 2.475 Y RBM48 n/a
9 TRCN0000130146 CAGGTTCAAGTGATTCTCCTA pLKO.1 6613 3UTR 100% 2.640 1.320 Y DICER1-AS1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011515017.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000465829 CCCTACCTAGCGGGTCATGTGAGA pLX_317 15.7% 93.5% 93.5% V5 (not translated due to frame shift) 1695_1696ins116 n/a
2 ccsbBroadEn_10494 pDONR223 100% 93.5% 93.5% None 1695_1696ins117 n/a
3 ccsbBroad304_10494 pLX_304 0% 93.5% 93.5% V5 1695_1696ins117 n/a
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