Construct: ORF TRCN0000466043
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012349.1_s317c1
- Derived from:
- ccsbBroadEn_05198
- DNA Barcode:
- TCTCGACATAGAGGAGGGGCTGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HACD2 (201562)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466043
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 201562 | HACD2 | 3-hydroxyacyl-CoA dehydrata... | NM_198402.5 | 100% | 100% | |
| 2 | human | 201562 | HACD2 | 3-hydroxyacyl-CoA dehydrata... | NM_001329783.2 | 91.6% | 91.6% | 274_342del |
| 3 | human | 201562 | HACD2 | 3-hydroxyacyl-CoA dehydrata... | XR_001740056.2 | 79.1% | 1_52del;735_875del;949_950insTTTGAA | |
| 4 | human | 201562 | HACD2 | 3-hydroxyacyl-CoA dehydrata... | NM_001329784.2 | 63.1% | 61.8% | (many diffs) |
| 5 | human | 201562 | HACD2 | 3-hydroxyacyl-CoA dehydrata... | XM_024453387.1 | 63.1% | 61.8% | (many diffs) |
| 6 | human | 201562 | HACD2 | 3-hydroxyacyl-CoA dehydrata... | NM_001329786.2 | 56.2% | 56.2% | 0_1ins333 |
| 7 | human | 201562 | HACD2 | 3-hydroxyacyl-CoA dehydrata... | NM_001329787.1 | 56.2% | 56.2% | 0_1ins333 |
| 8 | human | 201562 | HACD2 | 3-hydroxyacyl-CoA dehydrata... | XM_017005856.2 | 51% | 50.3% | (many diffs) |
| 9 | mouse | 70757 | Hacd2 | 3-hydroxyacyl-CoA dehydrata... | NM_023587.2 | 90.1% | 94.8% | (many diffs) |
| 10 | mouse | 70757 | Hacd2 | 3-hydroxyacyl-CoA dehydrata... | XM_006522570.3 | 50.7% | 54.3% | (many diffs) |
| 11 | mouse | 70757 | Hacd2 | 3-hydroxyacyl-CoA dehydrata... | XM_006522571.1 | 50.7% | 54.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 828
- ORF length:
- 762
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcagtggcg gcgactgcag cagcgaaggg gaatgggggc ggcggtggca 121 gggccggggc cggggacgcc agcggcacgc ggaagaagaa gggcccgggg cccctggcca 181 cggcgtacct ggtcatctac aatgtggtga tgacagccgg gtggctggtt atagcggttg 241 gtctggtccg agcatacctg gctaagggta gctaccatag cctttattat tcaattgaaa 301 agcctttgaa attctttcaa actggagcct tattggagat tttacattgt gctataggaa 361 ttgttccatc ttctgttgtc ctgacttctt tccaggtgat gtcaagagtt tttctaatat 421 gggcagtaac acatagcgtc aaagaggtac agagtgaaga cagtgtccTC CTGTTTGTTA 481 TTGCATGGAC GATCACGGAA ATCATCCGTT ACTCCTTTTA TACATTCAGT CTATTAAACC 541 ATCTGCCTTA CCTCATCAAA TGGGCCAGGT ACACACTTTT CATTGTGCTG TACCCAATGG 601 GAGTGTCAGG AGAACTGCTC ACAATATATG CAGCTCTGCC CTTTGTCAGA CAAGCTGGCC 661 TATATTCCAT CAGTTTACCC AACAAATACA ATTTCTCTTT TGACTACTAT GCATTCCTGA 721 TTCTAATAAT GATCTCCTAC ATTCCAATTT TTCCCCAGTT ATACTTCCAC ATGATACACC 781 AGAGAAGAAA GATCCTTTCT CATACTGAAG AACACAAGAA ATTTGAATAC CCAACTTTCT 841 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 901 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 961 GTGGAAAGGA CGATCTCGAC ATAGAGGAGG GGCTGACACG CGTTAAGTCg acaatcaacc 1021 tctggattac aaaatttgtg aaagatt