Construct: ORF TRCN0000466074
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013001.1_s317c1
- Derived from:
- ccsbBroadEn_02081
- DNA Barcode:
- AAAGAAGACGGCCCAATGCCACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- USP8 (9101)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466074
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9101 | USP8 | ubiquitin specific peptidase 8 | NM_001128610.3 | 100% | 100% | |
| 2 | human | 9101 | USP8 | ubiquitin specific peptidase 8 | NM_005154.5 | 100% | 100% | |
| 3 | human | 9101 | USP8 | ubiquitin specific peptidase 8 | XM_006720761.3 | 100% | 100% | |
| 4 | human | 9101 | USP8 | ubiquitin specific peptidase 8 | XM_011522193.3 | 100% | 100% | |
| 5 | human | 9101 | USP8 | ubiquitin specific peptidase 8 | XM_006720762.3 | 97.4% | 97.4% | 1803_1804ins87 |
| 6 | human | 9101 | USP8 | ubiquitin specific peptidase 8 | XM_017022718.1 | 97.4% | 97.4% | 1803_1804ins87 |
| 7 | human | 9101 | USP8 | ubiquitin specific peptidase 8 | XM_017022719.2 | 97.4% | 97.4% | 1803_1804ins87 |
| 8 | human | 9101 | USP8 | ubiquitin specific peptidase 8 | XM_017022720.2 | 97.4% | 97.4% | 1803_1804ins87 |
| 9 | human | 9101 | USP8 | ubiquitin specific peptidase 8 | NM_001283049.2 | 90.5% | 90.5% | 102_103ins231;1572_1573ins87 |
| 10 | human | 9101 | USP8 | ubiquitin specific peptidase 8 | XM_017022721.2 | 83% | 83% | 0_1ins570 |
| 11 | human | 9101 | USP8 | ubiquitin specific peptidase 8 | XM_017022722.1 | 83% | 83% | 0_1ins570 |
| 12 | human | 9101 | USP8 | ubiquitin specific peptidase 8 | XM_024450098.1 | 83% | 83% | 0_1ins570 |
| 13 | mouse | 84092 | Usp8 | ubiquitin specific peptidase 8 | NM_019729.3 | 81.6% | 81.7% | (many diffs) |
| 14 | mouse | 84092 | Usp8 | ubiquitin specific peptidase 8 | NM_001252580.1 | 80.9% | 81% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3423
- ORF length:
- 3354
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctgctgtg gcttcagttc ctaaagaact ctacctcagt tcttcactaa 121 aagaccttaa taagaagaca gaagttaaac cagagaaaat aagcactaag agttatgtgc 181 acagtgccct gaagatcttt aagacagcag aagaatgcag attagatcgt gatgaggaaa 241 gggcctatgt actatatatg aaatacgtga ctgtttataa tcttatcaaa aaaagacctg 301 atttcaagca acagcaggat tatttccatt caatacttgg acctggaaac atcaaaaaag 361 ctgtcgaaga agctgaaaga ctctctgaaa gccttaaatt aagatatgaa gaagctgaag 421 tccggaaaaa acttgaggaa aaagacaggc aggaggaagc acagcggcta caacaaaaaa 481 ggcaggaaac aggaagagag gatggtggca cattggctaa aggctctttg gagaatgttt 541 tggattccaa agacaaaacc caaaagagca atggtgaaaa gaatgaaaaa tgtgagacca 601 aagagaaagg agcaatcaca gcaaaggaac tatacacaat gatgacggat aaaaacatca 661 gcttgattat aatggatgct cgaagaatgc aggattatca ggattcctgt attttacatt 721 ctctcagtgt tcctgaagaa gccatcagtc caggagtcac tgctagttgg attgaagcac 781 acctgccaga tgattctaaa gacacatgga agaagagggg gaatgtggag tatgtggtac 841 ttcttgactg gtttagttct gccaaagatt tacagattgg aacaactctc cggagtctga 901 aagatgcact tttcaagtgg gaaagtaaaa ctgtcctgcg caatgagcct ttggttttag 961 agggaggcta tgaaaactgg ctcctttgtt atccccagta tacaacaaat gctaaggtca 1021 ctccaccccc acgacgccag aatgaagagg tgtctatctc attggatttt acttatccct 1081 cattggaaga atcaattcct tctaaacctg ctgcccagac gccacctgca tctatagaag 1141 tagatgaaaa tatagaattg ataagtggtc aaaatgagag aatgggacca ctgaatatat 1201 caactccagt tgaaccagtt gctgcttcta aatctgatgt ttcacccata attcagccag 1261 tgcctagtat aaagaatgtt ccacagattg atcgtactaa aaaaccagca gtcaaattgc 1321 ctgaagagca tagaataaaa tctgaaagta caaaccatga gcaacaatct cctcagagtg 1381 gaaaagttat tcctgatcgt tccaccaagc cagtagtttt ttctccaact ctcatgttaa 1441 cagatgaaga aaaggctcgt attcatgcag aaactgctct tctaatggaa aaaaacaaac 1501 aagaaaaaga acttcgggaa aggcagcaag aggaacagaa agagaaactg aggaaggaag 1561 aacaagaaca aaaagccaaa aagaaacaag aagctgaaga aaatgaaatt acagagaagc 1621 aacaaaaagc aaaagaagaa atggagaaga aagaaagtga acaggccaag aaagaagata 1681 aagaaacctc agcaaagagg ggcaaagaaa taacaggagt aaaaagacaa agtaaaagtg 1741 aacatgaaac ttctgatgcc aagaaatctg tagaagatag ggggaaaagg tgtccaaccc 1801 cagaaataca gaaaaagtca acaggagatg tgccccatac atctgtgaca ggggattcag 1861 gttcaggcaa gccatttaag attaaaggac aaccagaaag tggaattcta aggacaggaa 1921 cttttagaga ggatacagac gataccgaaa gaaataaagc tcaacgagaa cctttgacaa 1981 gagcacgaag tgaagaaatg gggaggatcg taccaggact gccttcaggc tgggccaagt 2041 ttcttgaccc aatcactgga acctttcgtt attatcattc acccaccaac actgttcata 2101 tgtacccacc ggaaatggct ccttcatctg cacctccttc cacccctcca actcataaag 2161 ccaagccaca gattcctgct gagcgggata gggaaccttc caaactgaag cgctcctact 2221 cctccccaga tataacccag gctattcaag aggaagagaa gaggaagcca acagtaactc 2281 caacagttaa tcgggaaaac aagccaacat gttatcctaa agctgagatc tcaaggcttt 2341 ctgcttctca gattcggaac ctcaatcctg tttttggagg ttctggacca gctcttactg 2401 gacttcgtaa cttaggaaat acttgttata tgaactcaat attgcagtgc ctatgtaacg 2461 ctccacattt ggctgattat ttcaaccgaa actgttatca ggatgatatt aacaggtcaa 2521 atttgttggg gcataaaggt gaagtggcag aagaatttgg tataatcatg aaagccctgt 2581 ggacaggaca gtatagatat atcagtccaa aggactttaa aatcaccatt gggaagatca 2641 atgaccagtt tgcaggatac agtcagcaag attcacaaga attgcttctg ttcctaatgg 2701 atggtctcca tgaagatcta aataaagctg ataatcggaa gagatataaa gaagaaaata 2761 atgatcatct cgatgacttt aaagctgcag aacatgcctg gcagaaacac aagcagctca 2821 atgagtctat tattgttgca ctttttcagg gtcaattcaa atctacagta cagtgcctca 2881 catgtcacaa aaagtctagg acatttgagg ccttcatgta tttgtctcta ccactagcat 2941 ccacaagtaa atgtacatta caggattgcc ttagattatt ttccaaagaa gaaaaactca 3001 cagataacaa cagattttac tgcagtcatt gcagagctcg acgggattct ctaaaaaaga 3061 tagaaatctg gaagttacCA CCTGTGCTTT TAGTGCATCT GAAACGTTTT TCCTACGATG 3121 GCAGGTGGAA ACAAAAATTA CAGACATCTG TGGACTTCCC GTTAGAAAAT CTTGACTTGT 3181 CACAGTATGT TATTGGTCCA AAGAACAATT TGAAGAAATA TAATTTGTTT TCTGTTTCAA 3241 ATCACTACGG TGGGCTGGAT GGAGGCCACT ACACAGCCTA TTGTAAAAAT GCAGCAAGAC 3301 AACGGTGGTT TAAGTTTGAT GATCATGAAG TTTCTGATAT CTCCGTTTCT TCTGTGAAAT 3361 CTTCAGCAGC TTATATCCTC TTTTATACTT CATTGGGACC ACGAGTAACT GATGTAGCCA 3421 CATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 3481 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 3541 GGCTTTATAT ATCTTGTGGA AAGGACGAAA AGAAGACGGC CCAATGCCAC ACACGCGTTA 3601 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt