Construct: ORF TRCN0000466083
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000620.1_s317c1
- Derived from:
- ccsbBroadEn_15256
- DNA Barcode:
- GTTTGTTCTAAACATTGCGACATC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF578 (147660)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466083
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 147660 | ZNF578 | zinc finger protein 578 | NM_001099694.2 | 37.2% | 37.1% | (many diffs) |
| 2 | human | 147660 | ZNF578 | zinc finger protein 578 | NM_001366182.1 | 37.2% | 37.1% | (many diffs) |
| 3 | human | 126017 | ZNF813 | zinc finger protein 813 | NM_001004301.4 | 31.2% | 28.8% | (many diffs) |
| 4 | human | 344787 | ZNF860 | zinc finger protein 860 | NM_001137674.3 | 30.5% | 25.7% | (many diffs) |
| 5 | human | 344787 | ZNF860 | zinc finger protein 860 | XM_017006299.1 | 30.5% | 25.7% | (many diffs) |
| 6 | human | 388561 | ZNF761 | zinc finger protein 761 | NM_001008401.4 | 25.3% | 22.6% | (many diffs) |
| 7 | human | 388561 | ZNF761 | zinc finger protein 761 | NM_001289951.2 | 25.3% | 22.6% | (many diffs) |
| 8 | human | 388561 | ZNF761 | zinc finger protein 761 | NM_001289952.1 | 25.3% | 22.6% | (many diffs) |
| 9 | human | 110116772 | ZNF765-ZNF761 | ZNF765-ZNF761 readthrough | NM_001350496.2 | 25.3% | 22.6% | (many diffs) |
| 10 | human | 55762 | ZNF701 | zinc finger protein 701 | XR_001753718.2 | 18.6% | (many diffs) | |
| 11 | human | 55762 | ZNF701 | zinc finger protein 701 | XR_001753717.2 | 15.7% | (many diffs) | |
| 12 | human | 55762 | ZNF701 | zinc finger protein 701 | XR_001753715.2 | 15.6% | (many diffs) | |
| 13 | human | 55762 | ZNF701 | zinc finger protein 701 | XR_001753716.2 | 14.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 732
- ORF length:
- 663
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagagaaaaa tctttccaat gtaatgagac tggcaaagcc tttaattgta 121 gctcatttgt aaggaaacat cagataatcc atttaggaga aaaacaatat aaatttgata 181 tatgtggcaa agtctttaat gagaagcgat accttgcacg ccatcgtaga tgtcacacta 241 gtgagaaacc ttacaagtgt aatgaatgtg gaaagtcctt cagttacaag tcatccctga 301 catgccatcg tagatgtcac actggtgaga aaccttacaa gtgtaatgaa tgtggaaagt 361 ccttcagtta caagtcatcc cttacatgcc atcataggtg tcacactggt gagaaacctt 421 acaagtgtaa tgaatgtgga aagtccttca gttacaagtc atcccttaga tgccatcgta 481 gacttcatac tggaataaaa CCTTACAAGT GTAATGAGTG TGGCAAGATG TTTGGTCAAA 541 ATTCAACCCT TGTAATTCAT AAGGCAATTC ATACTGGAGA GAAACCTTAC AAGTGTAATG 601 AATGTGGCAA GGCTTTTAAT CAACAATCAC ACCTTTCACG TCATCATAGA CTTCATACTG 661 GAGAGAAACC TTACAAGTGT AATGACTGTG GTAAGGCTTT TATTCATCAG TCAAGCCTTG 721 CATGTCATCA TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA 781 CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC 841 GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTTT GTTCTAAACA TTGCGACATC 901 ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt