Construct: ORF TRCN0000466300
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012118.1_s317c1
- Derived from:
- ccsbBroadEn_13162
- DNA Barcode:
- CGGCAGCAAGTCATAGCTCGCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCBLD2 (131566)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466300
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 131566 | DCBLD2 | discoidin, CUB and LCCL dom... | NM_080927.4 | 92.3% | 92.3% | (many diffs) |
| 2 | human | 131566 | DCBLD2 | discoidin, CUB and LCCL dom... | XM_024453347.1 | 89.9% | 90% | 0_1ins180;1400_1401ins42;1482C>T |
| 3 | human | 131566 | DCBLD2 | discoidin, CUB and LCCL dom... | XM_024453348.1 | 89.9% | 90% | 0_1ins180;1400_1401ins42;1482C>T |
| 4 | human | 131566 | DCBLD2 | discoidin, CUB and LCCL dom... | XM_011512419.2 | 82.6% | 82.7% | (many diffs) |
| 5 | mouse | 73379 | Dcbld2 | discoidin, CUB and LCCL dom... | NM_028523.3 | 79.6% | 79.5% | (many diffs) |
| 6 | mouse | 73379 | Dcbld2 | discoidin, CUB and LCCL dom... | XM_006522622.1 | 79.5% | 79.4% | (many diffs) |
| 7 | mouse | 73379 | Dcbld2 | discoidin, CUB and LCCL dom... | XM_011246022.1 | 75.9% | 70.9% | (many diffs) |
| 8 | mouse | 73379 | Dcbld2 | discoidin, CUB and LCCL dom... | XM_006522624.1 | 43.7% | 40.8% | (many diffs) |
| 9 | mouse | 73379 | Dcbld2 | discoidin, CUB and LCCL dom... | XR_001781870.1 | 27.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2295
- ORF length:
- 2229
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tctgttcctc ctgctcttac ttgtcctgct gctgctgctc gaggacgctg 121 gagcccagca aggtgatgga tgtggacaca ctgtactagg ccctgagagt ggaaccctta 181 catccataaa ctacccacag acctatccca acagcactgt ttgtgaatgg gagatccgtg 241 taaagatggg agagagagtt cgcatcaaat ttggtgactt tgacattgaa gattctgatt 301 cttgtcactt taattacttg agaatttata atggaattgg agtcagcaga actgaaatag 361 gcaaatactg tggtctgggg ttgcaaatga accattcaat tgaatcaaaa ggcaatgaaa 421 tcacattgct gttcatgagt ggaatccatg tttctggacg cggatttttg gcctcatact 481 ctgttataga taaacaagat ctaattactt gtttggacac tgcatccaat tttttggaac 541 ctgagttcag taagtactgc ccagctggtt gtctgcttcc ttttgctgag atatctggaa 601 caattcctca tggatataga gattcctcgc cattgtgcat ggctggtgtg catgcaggag 661 tagtgtcaaa cacgttgggc ggccaaatca gtgttgtaat tagtaaaggt atcccctatt 721 atgaaagttc tttggctaac aacgtcacat ctgtggtggg acacttatct acaagtcttt 781 ttacatttaa gacaagtgga tgttatggaa cactggggat ggagtctggt gtgatcgcgg 841 atcctcaaat aacagcatca tctgtgctgg agtggactga ccacacaggg caagagaaca 901 gttggaaacc caaaaaagcc aggctgaaaa aacctggacc gccttgggct gcttttgcca 961 ctgatgaata ccagtggtta caaatagatt tgaataagga aaagaaaata acaggcatta 1021 taaccactgg atccaccatg gtggagcaca attactatgt gtctgcctac agaatcctgt 1081 acagtgatga tgggcagaaa tggactgtgt acagagagcc tggtgtggag caagataaga 1141 tatttcaagg aaacaaagat tatcaccagg atgtgcgtaa taactttttg ccaccaatta 1201 ttgcacgttt tattagagtg aatcctaccc aatggcagca gaaaattgcc atgaaaatgg 1261 agctgctcgg atgtcagttt attcctaaag gtcgtcctcc aaaacttact caacctccac 1321 ctcctcggaa cagcaatgac ctcaaaaaca ctacagcccc tccaaaaata gccaaaggtc 1381 gtgccccaaa atttacgcaa ccactacaac ctcgcagtag caatgaattt cctgcacaga 1441 cagaacaaac aactgccagt cctgatatca gaaatactac cgtaactcca aatgtaacca 1501 aagatgtagc gctggctgca gttcttgtcc ctgtgctggt catggtcctc actactctca 1561 ttctcatatt agtgtgtgct tggcactgga gaaacagaaa gaaaaaaact gaaggcacct 1621 atgacttacc ttactgggac cgggcaggtt tttatttaat ggtctctttg gcttgccgtc 1681 acaatgaagg ttggtggaaa ggaatgaagc agtttcttcc tgcaaaagca gtggaccatg 1741 aggaaacccc agttcgctat agcagcagtg aagttaatca cctgagtcca agagaagtca 1801 ccacagtgct gcaggctgac tctgcagagt atgctcagcc actggtagga ggaattgttg 1861 gtacacttca tcaaagatct acctttaaac cagaagaagg aaaagaagca ggctatgcag 1921 acctagatcc TTACAACTCA CCAGGGCAGG AAGTTTATCA TGCCTATGCT GAACCACTCC 1981 CAATTACGGG GCCTGAGTAT GCAACCCCAA TCATCATGGA CATGTCAGGG CACCCCACAA 2041 CTTCAGTTGG TCAGCCCTCC ACATCCACTT TCAAGGCTAC GGGGAACCAA CCTCCCCCAC 2101 TAGTGGGAAC TTACAATACA CTTCTCTCCA GGACTGACAG CTGCTCCTCA GCCCAGGCCC 2161 AGTATGATAC CCCGAAAGCT GGGAAGCCAG GTCTACCTGC CCCAGACGAA TTGGTGTACC 2221 AGGTGCCACA GAGCACACAA GAAGTATCAG GAGCAGGAAG GGATGGGGAA TGTGATGTTT 2281 TTAAAGAAAT CCTTTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2341 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2401 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGGCAGCAAG TCATAGCTCG 2461 CCGGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt