Construct: ORF TRCN0000466447
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003180.1_s317c1
- Derived from:
- ccsbBroadEn_00359
- DNA Barcode:
- ATGTGTTTGCCGCACGCTGACCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPT1A (1374)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466447
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1374 | CPT1A | carnitine palmitoyltransfer... | NM_001031847.2 | 100% | 100% | |
| 2 | human | 1374 | CPT1A | carnitine palmitoyltransfer... | NM_001876.4 | 97.1% | 95.3% | (many diffs) |
| 3 | human | 1374 | CPT1A | carnitine palmitoyltransfer... | XM_017017220.1 | 97.1% | 95.3% | (many diffs) |
| 4 | human | 1374 | CPT1A | carnitine palmitoyltransfer... | XM_005273763.1 | 95.8% | 95.9% | 1_96del;1347T>C |
| 5 | human | 1374 | CPT1A | carnitine palmitoyltransfer... | XM_005273762.3 | 93.3% | 91.6% | (many diffs) |
| 6 | mouse | 12894 | Cpt1a | carnitine palmitoyltransfer... | NM_013495.2 | 79.8% | 82.8% | (many diffs) |
| 7 | mouse | 12894 | Cpt1a | carnitine palmitoyltransfer... | XM_006531654.2 | 79.8% | 82.8% | (many diffs) |
| 8 | mouse | 12894 | Cpt1a | carnitine palmitoyltransfer... | XM_006531655.2 | 79.8% | 82.8% | (many diffs) |
| 9 | mouse | 12894 | Cpt1a | carnitine palmitoyltransfer... | XM_006531658.3 | 79.8% | 82.8% | (many diffs) |
| 10 | mouse | 12894 | Cpt1a | carnitine palmitoyltransfer... | XM_006531659.2 | 79.8% | 82.8% | (many diffs) |
| 11 | mouse | 12894 | Cpt1a | carnitine palmitoyltransfer... | XM_011248602.2 | 77.3% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2334
- ORF length:
- 2268
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agaagctcac caagctgtgg cctttcagtt cacggtcact ccggacggga 121 ttgacctgcg gctgagccat gaagctctta gacaaatcta tctctctgga cttcattcct 181 ggaaaaagaa gttcatcaga ttcaagaacg gcatcatcac tggcgtgtac ccggcaagcc 241 cctccagttg gcttatcgtg gtggtgggcg tgatgacaac gatgtacgcc aagatcgacc 301 cctcgttagg aataattgca aaaatcaatc ggactctgga aacggccaac tgcatgtcca 361 gccagacgaa gaacgtggtc agcggcgtgc tgtttggcac cggcctgtgg gtggccctca 421 tcgtcaccat gcgctactcc ctgaaagtgc tgctctccta ccacgggtgg atgttcactg 481 agcacggcaa gatgagtcgt gccaccaaga tctggatggg tatggtcaag atcttttcag 541 gccgaaaacc catgttgtac agcttccaga catcgctgcc tcgcctgccg gtcccggctg 601 tcaaagacac tgtgaacagg tatctacagt cggtgaggcc tcttatgaag gaagaagact 661 tcaaacggat gacagcactt gctcaagatt ttgctgtcgg tcttggacca agattacagt 721 ggtatttgaa gttaaaatcc tggtgggcta caaattacgt gagcgactgg tgggaggagt 781 acatctacct ccgaggacga gggccgctca tggtgaacag caactattat gccatggatc 841 tgctgtatat ccttccaact cacattcagg cagcaagagc cggcaacgcc atccatgcca 901 tcctgcttta caggcgcaaa ctggaccggg aggaaatcaa accaattcgt cttttgggat 961 ccacgattcc actctgctcc gctcagtggg agcggatgtt taatacttcc cggatcccag 1021 gagaggagac agacaccatc cagcacatga gagacagcaa gcacatcgtc gtgtaccatc 1081 gaggacgcta cttcaaggtc tggctctacc atgatgggcg gctgctgaag ccccgggaga 1141 tggagcagca gatgcagagg atcctggaca atacctcgga gcctcagccc ggggaggcca 1201 ggctggcagc cctcaccgca ggagacagag ttccctgggc caggtgtcgt caggcctatt 1261 ttggacgtgg gaaaaataag cagtctcttg atgctgtgga gaaagcagcg ttcttcgtga 1321 cgttagatga aactgaagaa ggatacagaa gtgaagaccc ggatacgtca atggacagct 1381 acgccaaatc tctactacac ggccgatgtt acgacaggtg gtttgacaag tcgttcacgt 1441 ttgttgtctt caaaaacggg aagatgggcc tcaacgctga acactcctgg gcagatgcgc 1501 cgatcgtggc ccacctttgg gagtacgtca tgtccattga cagcctccag ctgggctatg 1561 cggaggatgg gcactgcaaa ggcgacatca atccgaacat tccgtacccc accaggctgc 1621 agtgggacat cccgggggaa tgtcaagagg ttatagagac ctccctgaac accgcaaatc 1681 ttctggcaaa cgacgtggat ttccattcct tcccattcgt agcctttggt aaaggaatca 1741 tcaagaaatg tcgcacgagc ccagacgcct ttgtgcagct ggccctccag ctggcgcact 1801 acaaggacat gggcaagttt tgcctcacat acgaggcctc catgacccgg ctcttccgag 1861 aggggaggac ggagaccgtg cgctcctgca ccactgagtc atgcgacTTC GTGCGGGCCA 1921 TGGTGGACCC GGCCCAGACG GTGGAACAGA GGCTGAAGTT GTTCAAGTTG GCGTCTGAGA 1981 AGCATCAGCA TATGTATCGC CTCGCCATGA CCGGCTCTGG GATCGATCGT CACCTCTTCT 2041 GCCTTTACGT GGTGTCTAAA TATCTCGCTG TGGAGTCCCC TTTCCTTAAG GAAGTTTTAT 2101 CTGAGCCTTG GAGATTATCA ACAAGCCAGA CCCCTCAGCA GCAAGTGGAG CTGTTTGACT 2161 TGGAGAATAA CCCAGAGTAC GTGTCCAGCG GAGGGGGCTT TGGACCGGTT GCTGATGACG 2221 GCTATGGTGT GTCGTACATC CTTGTGGGAG AGAACCTCAT CAATTTCCAC ATTTCTTCCA 2281 AGTTCTCTTG CCCTGAGACG GGGATTATAA GTCAAGGACC AAGTTCAGAT ACTTGCCCAA 2341 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2401 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2461 TATCTTGTGG AAAGGACGAA TGTGTTTGCC GCACGCTGAC CGCACGCGTT AAGTCgacaa 2521 tcaacctctg gattacaaaa tttgtgaaag att