Construct: ORF TRCN0000466542
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009805.1_s317c1
- Derived from:
- ccsbBroadEn_06266
- DNA Barcode:
- GAGATCCCGATCTCGGATACACAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCKR (2646)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466542
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2646 | GCKR | glucokinase regulator | NM_001486.4 | 99.9% | 99.8% | 1337T>C |
| 2 | human | 2646 | GCKR | glucokinase regulator | XM_017003796.1 | 69.5% | 69.4% | 0_1ins570;767T>C |
| 3 | human | 2646 | GCKR | glucokinase regulator | XM_017003797.1 | 69.5% | 69.4% | 0_1ins570;767T>C |
| 4 | human | 2646 | GCKR | glucokinase regulator | XR_001738699.1 | 59.6% | 1_66del;1029_1030ins175;1223_1224ins543 | |
| 5 | human | 2646 | GCKR | glucokinase regulator | XM_011532763.1 | 53.2% | 51.8% | 967_968ins98;999_1000ins778 |
| 6 | mouse | 231103 | Gckr | glucokinase regulatory protein | XM_006503881.3 | 85.2% | 87.4% | (many diffs) |
| 7 | mouse | 231103 | Gckr | glucokinase regulatory protein | XM_006503882.3 | 82.7% | 84.7% | (many diffs) |
| 8 | mouse | 231103 | Gckr | glucokinase regulatory protein | NM_144909.1 | 80.4% | 81.8% | (many diffs) |
| 9 | mouse | 231103 | Gckr | glucokinase regulatory protein | XM_006503883.3 | 78.1% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1944
- ORF length:
- 1875
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccaggcaca aaacggtttc aacatgtcat tgagaccccg gagcctggca 121 agtgggagtt gtctgggtac gaggcagctg tgccaatcac ggagaagtca aacccactga 181 cccaggatct agacaaagca gatgctgaga acattgttcg actgctaggg caatgtgatg 241 ctgagatctt ccaggaggag gggcaagccc tgtccacata ccagagactc tacagcgaat 301 ccattctgac caccatggta caggtggctg ggaaagttca ggaagtgctg aaggagccag 361 atggggggct ggttgtgctg agtggagggg gcacctctgg ccggatggca ttcctcatgt 421 cggtgtcctt taatcagctg atgaaaggtc tgggacagaa acctctttac acctacctca 481 ttgcaggtgg tgacaggtct gtggtggcct ctagggaggg gacagaagat agtgccttgc 541 acgggattga ggaactgaag aaggtggctg ccgggaagaa gagagtgatt gtcattggca 601 tttctgtggg actctctgct ccctttgtgg caggccagat ggactgctgc atgaacaaca 661 cagctgtctt cttgccagtc ctggttggct tcaatccagt gagcatggcc agaaatgacc 721 ccattgaaga ctggagttca acattccgac aagtagcaga gcggatgcag aaaatgcagg 781 agaaacagaa agcttttgtg ctcaatcctg ccatcgggcc cgagggtctc agcggctcct 841 cccggatgaa aggtggaagt gccaccaaga ttctgctgga aaccctgtta ttagcagccc 901 ataagactgt ggaccagggc attgcagcat ctcaaagatg cctcctggaa atcttgcgga 961 catttgagcg agctcatcag gtgacctaca gccaaagccc caagattgcc accctgatga 1021 agagtgtcag caccagtctg gagaagaaag gccacgtgta cctggttggc tggcagaccc 1081 tgggcatcat tgccatcatg gatggagtag agtgcatcca cacctttggt gctgatttcc 1141 gagatgtccg tggctttctc attggtgatc acagtgacat gtttaaccag aaggctgagc 1201 tcaccaacca gggtccccag ttcaccttct cccaggagga cttcctgact tccatccttc 1261 cctctctcac ggaaatcgat actgtggtct tcattttcac cctggatgac aacctcacgg 1321 aggtgcagac tatagtggag caggtgaaag agaagaccaa ccacatccag gccctggcac 1381 acagcaccgt gggtcagacc ttgccgatcc ctctgaagaa gctctttccc tccatcatca 1441 gcatcacatg gccactgctt ttctttgaat atgaagggaa cttcatccag aagttccagc 1501 gtgagctaag caccaaatgg gtgctgaata cagtgagtac aggtgctcat gtgcttcttg 1561 gtaagatcct acaaaaccac atgttggacc TTCGGATTAG CAACTCCAAG CTCTTCTGGC 1621 GGGCGCTGGC CATGCTGCAG CGGTTCTCTG GACAGTCCAA GGCTCGATGC ATCGAGAGCC 1681 TCCTCCGAGC GATCCACTTT CCCCAGCCAC TGTCAGATGA TATTCGGGCT GCTCCCATCT 1741 CCTGCCATGT CCAGGTTGCA CATGAGAAGG AACAGGTGAT ACCCATCGCC TTGCTGAGCC 1801 TCCTATTCCG GTGCTCGATC ACTGAGGCTC AGGCACACCT GGCTGCAGCT CCTTCTGTCT 1861 GTGAGGCTGT CAGGAGTGCT CTTGCTGGGC CAGGTCAGAA GCGCACTGCG GACCCCCTCG 1921 AGATCCTAGA GCCTGACGTT CAGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1981 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2041 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG AGATCCCGAT 2101 CTCGGATACA CAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2161 att