Construct: ORF TRCN0000466607
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003181.1_s317c1
- Derived from:
- ccsbBroadEn_09588
- DNA Barcode:
- ACTCGATAAAACTAGGGCTATTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APOBEC3D (140564)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466607
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | NM_152426.4 | 99.9% | 100% | 963C>T |
| 2 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | NM_145298.6 | 86.9% | 77.4% | (many diffs) |
| 3 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XM_024452179.1 | 86.9% | 77.4% | (many diffs) |
| 4 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | XM_017028596.2 | 84.7% | 84.8% | 489_695del;1170C>T |
| 5 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XM_024452178.1 | 73.7% | 65.7% | (many diffs) |
| 6 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | NM_001363781.1 | 52.2% | 52.3% | 208_209ins552;411C>T |
| 7 | human | 27350 | APOBEC3C | apolipoprotein B mRNA editi... | NM_014508.3 | 44.4% | 37.5% | (many diffs) |
| 8 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | XR_001755170.2 | 40.8% | (many diffs) | |
| 9 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | XR_001755169.2 | 37.7% | (many diffs) | |
| 10 | human | 27350 | APOBEC3C | apolipoprotein B mRNA editi... | XM_024452218.1 | 34% | 26% | (many diffs) |
| 11 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XR_002958675.1 | 19.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1224
- ORF length:
- 1158
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tgggcatgaa tccacagatc agaaatccga tggagcggat gtatcgagac acattctacg 121 acaactttga aaacgaaccc atcctctatg gtcggagcta cacttggctg tgctatgaag 181 tgaaaataaa gaggggccgc tcaaatctcc tttgggacac aggggtcttt cgaggcccgg 241 tactacccaa acgtcagtcg aatcacaggc aggaggtgta tttccggttt gagaaccacg 301 cagaaatgtg cttcttatct tggttctgtg gcaaccgact gcctgctaac aggcgcttcc 361 agatcacctg gtttgtatca tggaacccct gcctgccctg tgtggtgaag gtgaccaaat 421 tcttggctga gcaccccaat gtcaccctga ccatctctgc cgcccgcctc tactactacc 481 gggatagaga ttggcggtgg gtgctcctca ggctgcataa ggcaggggcc cgtgtgaaga 541 tcatggacta tgaagacttt gcatactgct gggaaaactt tgtgtgcaat gaaggtcagc 601 cattcatgcc ttggtacaaa ttcgatgaca attatgcatc cctgcaccgc acgctaaagg 661 agattctcag aaacccgatg gaggcaatgt acccacacat attctacttc cactttaaaa 721 acctactgaa agcctgtggt cggaacgaaa gctggctgtg cttcaccatg gaagttacaa 781 agcaccactc agctgtcttc cggaagaggg gcgtcttccg aaaccaggtg gatccTGAGA 841 CCCATTGTCA TGCAGAAAGG TGCTTCCTCT CTTGGTTCTG TGACGACATA CTGTCTCCTA 901 ACACAAACTA CGAGGTCACC TGGTACACAT CTTGGAGCCC TTGCCCAGAG TGTGCAGGGG 961 AGGTGGCCGA GTTCCTGGCC AGGCACAGCA ACGTGAATCT CACCATCTTC ACCGCCCGCC 1021 TCTGCTATTT CTGGGATACA GATTACCAGG AGGGGCTCTG CAGCCTGAGT CAGGAAGGGG 1081 CCTCCGTGAA GATCATGGGC TACAAAGATT TTGTATCTTG TTGGAAAAAC TTTGTGTACA 1141 GTGATGATGA GCCATTCAAG CCTTGGAAGG GACTACAAAC CAACTTTCGA CTTCTGAAAA 1201 GAAGGCTACG GGAGATTCTC CAGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1261 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1321 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CTCGATAAAA 1381 CTAGGGCTAT TACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1441 att