Construct: ORF TRCN0000466980
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009128.1_s317c1
- Derived from:
- ccsbBroadEn_09043
- DNA Barcode:
- ATAGACGTTACGCATTAGAGGAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSEN2 (80746)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466980
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | NM_001145392.1 | 99.9% | 100% | 12A>T |
| 2 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | NM_001321277.1 | 99.9% | 100% | 12A>T |
| 3 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | NM_025265.4 | 99.9% | 100% | 12A>T |
| 4 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XM_017007296.2 | 99.9% | 100% | 12A>T |
| 5 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XM_017007292.1 | 96% | 96% | 12A>T;307_363del |
| 6 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XM_017007293.1 | 96% | 96% | 12A>T;307_363del |
| 7 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XM_017007294.1 | 96% | 96% | 12A>T;307_363del |
| 8 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XM_017007295.2 | 96% | 96% | 12A>T;307_363del |
| 9 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | NM_001145393.2 | 94.3% | 94.4% | 12A>T;831_832ins78 |
| 10 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | NM_001321279.1 | 94.3% | 94.4% | 12A>T;831_832ins78 |
| 11 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XM_024453785.1 | 94.3% | 94.4% | 12A>T;831_832ins78 |
| 12 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XM_017007297.1 | 90.6% | 90.7% | 12A>T;307_363del;888_889ins78 |
| 13 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | NM_001145394.1 | 87.2% | 87.3% | 12A>T;515_516ins177 |
| 14 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | NM_001321278.2 | 87.1% | 86.7% | (many diffs) |
| 15 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XM_024453784.1 | 86.7% | 86.3% | (many diffs) |
| 16 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XM_011534139.3 | 80.4% | 80.4% | 0_1ins273 |
| 17 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | NR_135607.2 | 70.7% | 1_55del;67A>T;1451_1969del | |
| 18 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_002959593.1 | 68% | 1_135del;147A>T;1531_2047del | |
| 19 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_002959592.1 | 66.6% | (many diffs) | |
| 20 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740290.2 | 66% | (many diffs) | |
| 21 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740301.2 | 65.7% | (many diffs) | |
| 22 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740294.2 | 65.3% | (many diffs) | |
| 23 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_002959594.1 | 64.1% | (many diffs) | |
| 24 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740288.2 | 63.3% | (many diffs) | |
| 25 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740300.2 | 63.2% | (many diffs) | |
| 26 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740303.2 | 61.6% | (many diffs) | |
| 27 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740295.2 | 61.5% | (many diffs) | |
| 28 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740302.2 | 61.5% | (many diffs) | |
| 29 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740289.2 | 60.7% | 1_387del;399A>T;1783_2295del | |
| 30 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740287.1 | 59.1% | (many diffs) | |
| 31 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_427296.2 | 57.9% | (many diffs) | |
| 32 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740293.1 | 57.5% | (many diffs) | |
| 33 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740291.1 | 57.1% | (many diffs) | |
| 34 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740292.1 | 55.5% | (many diffs) | |
| 35 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_002959595.1 | 55.4% | (many diffs) | |
| 36 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740297.1 | 54.2% | (many diffs) | |
| 37 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740304.1 | 53.7% | (many diffs) | |
| 38 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740298.2 | 51.1% | (many diffs) | |
| 39 | human | 80746 | TSEN2 | tRNA splicing endonuclease ... | XR_001740296.1 | 49.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1461
- ORF length:
- 1395
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agaagctgtt ttccatgccc caaagaggaa aagaagagtg tatgagactt 121 acgagtctcc attgccaatc ccttttggtc aggaccatgg tcctctgaaa gaattcaaga 181 tattccgtgc tgaaatgatt aacaacaatg tgattgtgag gaatgcggag gacattgagc 241 agctctatgg gaaaggttat tttggaaaag gtattctttc aagaagccgt ccaagcttca 301 caatttcaga tcctaaactg gttgctaaat ggaaagatat gaagacaaac atgcctatca 361 tcacatcaaa gaggtatcag catagtgttg agtgggcagc agagctgatg cgtagacagg 421 ggcaggatga gagtacagtg cgcagaatcc tcaaggatta cacgaaaccg cttgagcatc 481 ctcctgtgaa aaggaatgaa gaggctcaag tgcatgacaa gcttaactct ggaatggttt 541 ccaacatgga aggcacagca gggggagaga gaccttctgt ggtaaacggg gactctggaa 601 agtcaggtgg tgtgggtgat ccccgtgagc cattaggctg cctgcaggag ggctctggct 661 gccacccaac aacagagagc tttgagaaaa gcgtgcgaga ggatgcctca cctctgcccc 721 atgtctgttg ctgcaaacaa gatgctctca tcctccagcg tggccttcat catgaagacg 781 gcagccagca catcggcctc ctgcatcctg gggacagagg gcctgaccat gagtacgtgc 841 tggtcgagga agcggagtgt gccatgagcg agagggaggc tgccccaaat gaggaattgg 901 tgcaaagaaa caggttaata tgcagaagaa atccatatag gatctttgag tatttgcaac 961 tcagcctaga agaggccttt ttcttggtct atgctctggg atgtttaagt atttactatg 1021 agaaggagcc tttaacgata gtgaagctct ggaaagcttt cactgtagtt cagcccacgt 1081 tcagaaccac ctacatggcc taccattact ttcgaagcaa gggctgggTG CCCAAAGTGG 1141 GACTCAAGTA CGGGACAGAT TTACTGCTAT ATCGGAAAGG CCCTCCATTT TACCATGCAA 1201 GTTATTCTGT CATTATCGAG CTAGTTGATG ACCATTTTGA AGGCTCTCTC CGCAGGCCTC 1261 TCAGTTGGAA GTCCCTGGCT GCCTTGAGCA GAGTTTCCGT TAATGTCTCT AAGGAACTTA 1321 TGCTGTGCTA TTTGATTAAA CCCTCTACTA TGACTGACAA GGAAATGGAG TCACCAGAAT 1381 GTATGAAAAG GATTAAAGTT CAGGAGGTGA TTCTGAGTCG ATGGGTTTCT TCACGAGAGA 1441 GGAGTGACCA AGACGATCTT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1501 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1561 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATAG ACGTTACGCA 1621 TTAGAGGAGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt