Construct: ORF TRCN0000467014
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007581.1_s317c1
- Derived from:
- ccsbBroadEn_13244
- DNA Barcode:
- TTGCCATGATGAACCCCCTTACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPM3P9 (147804)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467014
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7170 | TPM3 | tropomyosin 3 | NM_001278191.2 | 52.4% | 46.8% | (many diffs) |
| 2 | human | 7170 | TPM3 | tropomyosin 3 | NM_001278188.2 | 47.8% | 47.8% | (many diffs) |
| 3 | human | 7170 | TPM3 | tropomyosin 3 | NM_153649.4 | 35% | 35% | (many diffs) |
| 4 | human | 7170 | TPM3 | tropomyosin 3 | NM_001043351.2 | 33.5% | 29.8% | (many diffs) |
| 5 | human | 7170 | TPM3 | tropomyosin 3 | NM_001364680.2 | 30.5% | 30.5% | (many diffs) |
| 6 | human | 7170 | TPM3 | tropomyosin 3 | NM_001278189.2 | 29.4% | 27% | (many diffs) |
| 7 | human | 7170 | TPM3 | tropomyosin 3 | NM_001364683.1 | 29.4% | 27% | (many diffs) |
| 8 | human | 7170 | TPM3 | tropomyosin 3 | NM_001364679.2 | 29.1% | 25.9% | (many diffs) |
| 9 | human | 7170 | TPM3 | tropomyosin 3 | NM_001278190.1 | 27.5% | 27.8% | (many diffs) |
| 10 | human | 7170 | TPM3 | tropomyosin 3 | NM_001364682.1 | 25.7% | 23.5% | (many diffs) |
| 11 | human | 147804 | TPM3P9 | tropomyosin 3 pseudogene 9 | NR_003148.3 | 9.3% | (many diffs) | |
| 12 | human | 7170 | TPM3 | tropomyosin 3 | NR_103461.2 | 8% | (many diffs) | |
| 13 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_006501841.3 | 46.3% | 47.8% | (many diffs) |
| 14 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | NM_001253738.1 | 34% | 35% | (many diffs) |
| 15 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | NM_001253740.1 | 31.2% | 29.8% | (many diffs) |
| 16 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | NM_022314.3 | 29.5% | 30.5% | (many diffs) |
| 17 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | NM_001271764.1 | 29.5% | 27% | (many diffs) |
| 18 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_017319677.1 | 29.5% | 27% | (many diffs) |
| 19 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_006501835.2 | 28.8% | 26.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 342
- ORF length:
- 276
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGA TGAGCAGATC AGACTGATGG ACCAGAACCT GAAGTGTCTG AGTGCAGCTG 121 AAGAAAAGTA CTCTCAAAAA GAAGACAAAT GTGAGGAAGA GAGGAAGATT CTTACTGATA 181 ATCTTAAGGA GGCAGAGACC CATGCTGAGT TGGCTGAGAG ATCAGTAGCC AAGCTGGAAA 241 AGACAATTGA TGACTTGGAA GATAAACTGA AATGCACCAA AGAGGAACAC CTCTGTACAC 301 AAAGGATGCT GGACCAGACT CTGCTTGACC TGAATGAGAT GTACCCAACT TTCTTGTACA 361 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 421 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 481 AGGACGATTG CCATGATGAA CCCCCTTACC AACGCGTTAA GTCgacaatc aacctctgga 541 ttacaaaatt tgtgaaagat t