Construct: ORF TRCN0000467117
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001068.1_s317c1
- Derived from:
- ccsbBroadEn_06235
- DNA Barcode:
- ATGTGTCCCAATCTGATGCATCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- G6PD (2539)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467117
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2539 | G6PD | glucose-6-phosphate dehydro... | NM_001042351.3 | 100% | 100% | |
| 2 | human | 2539 | G6PD | glucose-6-phosphate dehydro... | NM_001360016.2 | 100% | 100% | |
| 3 | human | 2539 | G6PD | glucose-6-phosphate dehydro... | NM_000402.4 | 94.4% | 94.4% | 1_90del |
| 4 | mouse | 14381 | G6pdx | glucose-6-phosphate dehydro... | NM_008062.2 | 87.5% | 93.7% | (many diffs) |
| 5 | mouse | 14380 | G6pd2 | glucose-6-phosphate dehydro... | NM_019468.2 | 84.7% | 86.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1611
- ORF length:
- 1545
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agagcaggtg gccctgagcc ggacccaggt gtgcgggatc ctgcgggaag 121 agcttttcca gggcgatgcc ttccatcagt cggatacaca catattcatc atcatgggtg 181 catcgggtga cctggccaag aagaagatct accccaccat ctggtggctg ttccgggatg 241 gccttctgcc cgaaaacacc ttcatcgtgg gctatgcccg ttcccgcctc acagtggctg 301 acatccgcaa acagagtgag cccttcttca aggccacccc agaggagaag ctcaagctgg 361 aggacttctt tgcccgcaac tcctatgtgg ctggccagta cgatgatgca gcctcctacc 421 agcgcctcaa cagccacatg aatgccctcc acctggggtc acaggccaac cgcctcttct 481 acctggcctt gcccccgacc gtctacgagg ccgtcaccaa gaacattcac gagtcctgca 541 tgagccagat aggctggaac cgcatcatcg tggagaagcc cttcgggagg gacctgcaga 601 gctctgaccg gctgtccaac cacatctcct ccctgttccg tgaggaccag atctaccgca 661 tcgaccacta cctgggcaag gagatggtgc agaacctcat ggtgctgaga tttgccaaca 721 ggatcttcgg ccccatctgg aaccgggaca acatcgcctg cgttatcctc accttcaagg 781 agccctttgg cactgagggt cgcgggggct atttcgatga atttgggatc atccgggacg 841 tgatgcagaa ccacctactg cagatgctgt gtctggtggc catggagaag cccgcctcca 901 ccaactcaga tgacgtccgt gatgagaagg tcaaggtgtt gaaatgcatc tcagaggtgc 961 aggccaacaa tgtggtcctg ggccagtacg tggggaaccc cgatggagag ggcgaggcca 1021 ccaaagggta cctggacgac cccacggtgc cccgcgggtc caccaccgcc acttttgcag 1081 ccgtcgtcct ctatgtggag aatgagaggt gggatggggt gcccttcatc ctgcgctgcg 1141 gcaaggcccT GAACGAGCGC AAGGCCGAGG TGAGGCTGCA GTTCCATGAT GTGGCCGGCG 1201 ACATCTTCCA CCAGCAGTGC AAGCGCAACG AGCTGGTGAT CCGCGTGCAG CCCAACGAGG 1261 CCGTGTACAC CAAGATGATG ACCAAGAAGC CGGGCATGTT CTTCAACCCC GAGGAGTCGG 1321 AGCTGGACCT GACCTACGGC AACAGATACA AGAACGTGAA GCTCCCTGAC GCCTATGAGC 1381 GCCTCATCCT GGACGTCTTC TGCGGGAGCC AGATGCACTT CGTGCGCAGC GACGAGCTCC 1441 GTGAGGCCTG GCGTATTTTC ACCCCACTGC TGCACCAGAT TGAGCTGGAG AAGCCCAAGC 1501 CCATCCCCTA TATTTATGGC AGCCGAGGCC CCACGGAGGC AGACGAGCTG ATGAAGAGAG 1561 TGGGTTTCCA GTATGAGGGC ACCTACAAGT GGGTGAACCC CCACAAGCTC TGCCCAACTT 1621 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1681 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1741 CTTGTGGAAA GGACGAATGT GTCCCAATCT GATGCATCTC ACGCGTTAAG TCgacaatca 1801 acctctggat tacaaaattt gtgaaagatt