Construct: ORF TRCN0000467477
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015822.2_s317c1
- Derived from:
- ccsbBroadEn_01527
- DNA Barcode:
- GTCCCTAAAGATCAAAATTTACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SGK1 (6446)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467477
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6446 | SGK1 | serum/glucocorticoid regula... | NM_005627.4 | 100% | 100% | |
| 2 | human | 6446 | SGK1 | serum/glucocorticoid regula... | NM_001143678.2 | 93.8% | 90.9% | (many diffs) |
| 3 | human | 6446 | SGK1 | serum/glucocorticoid regula... | NM_001143677.2 | 91.2% | 87.2% | (many diffs) |
| 4 | human | 6446 | SGK1 | serum/glucocorticoid regula... | NM_001291995.2 | 89.7% | 89.7% | 413_414ins132 |
| 5 | human | 6446 | SGK1 | serum/glucocorticoid regula... | NM_001143676.1 | 80.3% | 75.2% | (many diffs) |
| 6 | human | 6446 | SGK1 | serum/glucocorticoid regula... | XM_011536071.1 | 80.3% | 75.2% | (many diffs) |
| 7 | mouse | 20393 | Sgk1 | serum/glucocorticoid regula... | NM_011361.3 | 88.7% | 96.5% | (many diffs) |
| 8 | mouse | 20393 | Sgk1 | serum/glucocorticoid regula... | NM_001161849.2 | 85.6% | 91.8% | (many diffs) |
| 9 | mouse | 20393 | Sgk1 | serum/glucocorticoid regula... | NM_001161850.2 | 83.9% | 88.2% | (many diffs) |
| 10 | mouse | 20393 | Sgk1 | serum/glucocorticoid regula... | NM_001161847.2 | 83.6% | 90.9% | (many diffs) |
| 11 | mouse | 20393 | Sgk1 | serum/glucocorticoid regula... | NM_001161848.2 | 83.6% | 90.9% | (many diffs) |
| 12 | mouse | 20393 | Sgk1 | serum/glucocorticoid regula... | NM_001161845.2 | 71.9% | 72.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1359
- ORF length:
- 1293
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ggtgaaaact gaggctgcta agggcaccct cacttactcc aggatgaggg 121 gcatggtggc aattctcatc gctttcatga agcagaggag gatgggtctg aacgacttta 181 ttcagaagat tgccaataac tcctatgcat gcaaacaccc tgaagttcag tccatcttga 241 agatctccca acctcaggag cctgagctta tgaatgccaa cccttctcct ccaccaagtc 301 cttctcagca aatcaacctt ggcccgtcgt ccaatcctca tgctaaacca tctgactttc 361 acttcttgaa agtgatcgga aagggcagtt ttggaaaggt tcttctagca agacacaagg 421 cagaagaagt gttctatgca gtcaaagttt tacagaagaa agcaatcctg aaaaagaaag 481 aggagaagca tattatgtcg gagcggaatg ttctgttgaa gaatgtgaag caccctttcc 541 tggtgggcct tcacttctct ttccagactg ctgacaaatt gtactttgtc ctagactaca 601 ttaatggtgg agagttgttc taccatctcc agagggaacg ctgcttcctg gaaccacggg 661 ctcgtttcta tgctgctgaa atagccagtg ccttgggcta cctgcattca ctgaacatcg 721 tttatagaga cttaaaacca gagaatattt tgctagattc acagggacac attgtcctta 781 ctgacttcgg actctgcaag gagaacattg aacacaacag cacaacatcc accttctgtg 841 gcacgccgga gtatctcgca cctgaggtgc ttcataagca gCCTTATGAC AGGACTGTGG 901 ACTGGTGGTG CCTGGGAGCT GTCTTGTATG AGATGCTGTA TGGCCTGCCG CCTTTTTATA 961 GCCGAAACAC AGCTGAAATG TACGACAACA TTCTGAACAA GCCTCTCCAG CTGAAACCAA 1021 ATATTACAAA TTCCGCAAGA CACCTCCTGG AGGGCCTCCT GCAGAAGGAC AGGACAAAGC 1081 GGCTCGGGGC CAAGGATGAC TTCATGGAGA TTAAGAGTCA TGTCTTCTTC TCCTTAATTA 1141 ACTGGGATGA TCTCATTAAT AAGAAGATTA CTCCCCCTTT TAACCCAAAT GTGAGTGGGC 1201 CCAACGACCT ACGGCACTTT GACCCCGAGT TTACCGAAGA GCCTGTCCCC AACTCCATTG 1261 GCAAGTCCCC TGACAGCGTC CTCGTCACAG CCAGCGTCAA GGAAGCTGCC GAGGCTTTCC 1321 TAGGCTTTTC CTATGCGCCT CCCACGGACT CTTTCCTCTG CCCAACTTTC TTGTACAAAG 1381 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1441 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1501 ACGAGTCCCT AAAGATCAAA ATTTACCTAC GCGTTAAGTC gacaatcaac ctctggatta 1561 caaaatttgt gaaagatt