Transcript: Mouse NM_011361.3

Mus musculus serum/glucocorticoid regulated kinase 1 (Sgk1), transcript variant 6, mRNA.

Source:
NCBI, updated 2017-06-26
Taxon:
Mus musculus (mouse)
Gene:
Sgk1 (20393)
Length:
2471
CDS:
85..1380

Additional Resources:

NCBI RefSeq record:
NM_011361.3
NBCI Gene record:
Sgk1 (20393)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146486 AACTTACTGTTTGCATGCAT pXPR_003 AGG 142 11% 2 -0.6173 Sgk1 SGK1 77459
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_011361.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000271153 TGGAGATTAAGAGTCATATTT pLKO_005 1124 CDS 100% 15.000 21.000 N Sgk1 n/a
2 TRCN0000022885 CGGCTGAGATGTACGACAATA pLKO.1 989 CDS 100% 13.200 18.480 N Sgk1 n/a
3 TRCN0000022884 GCATATTATGTCAGAGCGGAA pLKO.1 507 CDS 100% 2.160 3.024 N Sgk1 n/a
4 TRCN0000271155 ACTCCCTAAACATCGTTTATA pLKO_005 725 CDS 100% 15.000 12.000 N Sgk1 n/a
5 TRCN0000040175 CGGAATGTTCTGTTGAAGAAT pLKO.1 523 CDS 100% 5.625 4.500 N SGK1 n/a
6 TRCN0000022886 GCCTCTCCAGTTGAAACCAAA pLKO.1 1020 CDS 100% 4.950 3.960 N Sgk1 n/a
7 TRCN0000271154 GTCTGTACATTGGGTTATAAC pLKO_005 2270 3UTR 100% 13.200 9.240 N Sgk1 n/a
8 TRCN0000284338 TGGGATGATCTCATCAATAAG pLKO_005 1162 CDS 100% 13.200 9.240 N Sgk1 n/a
9 TRCN0000022887 GCATGCAAACACGCTGAAGTT pLKO.1 226 CDS 100% 4.950 3.465 N Sgk1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_011361.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01527 pDONR223 100% 88.7% 96.5% None (many diffs) n/a
2 ccsbBroad304_01527 pLX_304 52.3% 88.7% 96.5% V5 (many diffs) n/a
3 TRCN0000467477 GTCCCTAAAGATCAAAATTTACCT pLX_317 37.1% 88.7% 96.5% V5 (many diffs) n/a
4 ccsbBroadEn_14840 pDONR223 0% 88.7% 96.5% None (many diffs) n/a
5 ccsbBroad304_14840 pLX_304 0% 88.7% 96.5% V5 (many diffs) n/a
6 TRCN0000473658 TATATATCTACAATCTCAGAACCC pLX_317 36.7% 88.6% 96.2% V5 (many diffs) n/a
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