Transcript: Human NM_020445.6

Homo sapiens actin related protein 3B (ACTR3B), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
ACTR3B (57180)
Length:
2213
CDS:
132..1388

Additional Resources:

NCBI RefSeq record:
NM_020445.6
NBCI Gene record:
ACTR3B (57180)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_020445.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000108329 CCAGGTGGTCACGCATCACAT pLKO.1 1226 CDS 100% 1.650 2.310 N ACTR3B n/a
2 TRCN0000108325 GCTGACAAATACAATTCTGAA pLKO.1 1647 3UTR 100% 4.950 3.465 N ACTR3B n/a
3 TRCN0000108326 CCGCTGTATAAGAATGTCGTA pLKO.1 1071 CDS 100% 2.640 1.848 N ACTR3B n/a
4 TRCN0000108328 GCCATCGATAAACCTACATAT pLKO.1 327 CDS 100% 13.200 6.600 Y ACTR3B n/a
5 TRCN0000418383 CGATACGACATGGAATCATTG pLKO_005 361 CDS 100% 10.800 5.400 Y ACTR3B n/a
6 TRCN0000434511 TCATTGAAGACTGGGATCTTA pLKO_005 376 CDS 100% 5.625 2.813 Y ACTR3B n/a
7 TRCN0000414318 AGTTCATTATTCCTTCATGTA pLKO_005 214 CDS 100% 4.950 2.475 Y ACTR3B n/a
8 TRCN0000423092 TAGGAGATGAAGCCATCGATA pLKO_005 316 CDS 100% 4.950 2.475 Y ACTR3B n/a
9 TRCN0000108327 CCAGGACTCTACATTGCAGTT pLKO.1 540 CDS 100% 4.050 2.025 Y ACTR3B n/a
10 TRCN0000414906 GAGTCAGCAAAGGTAGTTGAC pLKO_005 246 CDS 100% 4.050 2.025 Y ACTR3B n/a
11 TRCN0000090296 CCAGGACTCTACATTGCAGTA pLKO.1 540 CDS 100% 4.050 2.025 Y Actr3b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_020445.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03803 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_03803 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000480353 AATTTTCGGGTCTCCGCCAGAGAG pLX_317 33.6% 100% 100% V5 n/a
4 ccsbBroadEn_03802 pDONR223 100% 83.2% 83.2% None 952_1161del n/a
5 ccsbBroad304_03802 pLX_304 0% 83.2% 83.2% V5 952_1161del n/a
6 TRCN0000491360 AAGTAAGAACTACCAGACTCACCT pLX_317 33.1% 83.2% 83.2% V5 952_1161del n/a
7 ccsbBroadEn_02313 pDONR223 100% 76.8% 90.6% None (many diffs) n/a
8 ccsbBroad304_02313 pLX_304 0% 76.8% 90.6% V5 (many diffs) n/a
9 TRCN0000467535 CCCCTGACTGGTTGATGGTACCGA pLX_317 28.8% 76.8% 90.6% V5 (many diffs) n/a
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