Construct: ORF TRCN0000467714
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007865.1_s317c1
- Derived from:
- ccsbBroadEn_00920
- DNA Barcode:
- ATTAGCGGGGACGAAACGTTTAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT31 (3881)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467714
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3881 | KRT31 | keratin 31 | NM_002277.3 | 100% | 100% | |
| 2 | human | 3884 | KRT33B | keratin 33B | NM_002279.5 | 91.2% | 91.3% | (many diffs) |
| 3 | human | 3883 | KRT33A | keratin 33A | NM_004138.4 | 90% | 90.8% | (many diffs) |
| 4 | human | 100653049 | LOC100653049 | keratin, type I cuticular Ha4 | XM_011547005.2 | 81.9% | 80.6% | (many diffs) |
| 5 | human | 3885 | KRT34 | keratin 34 | XM_011524793.2 | 81.9% | 80.4% | (many diffs) |
| 6 | human | 100653049 | LOC100653049 | keratin, type I cuticular Ha4 | XM_003403882.5 | 77.6% | 76.4% | (many diffs) |
| 7 | human | 3885 | KRT34 | keratin 34 | NM_021013.3 | 77.6% | 76.2% | (many diffs) |
| 8 | human | 3883 | KRT33A | keratin 33A | XM_011524786.3 | 50.5% | 48.2% | (many diffs) |
| 9 | mouse | 16660 | Krt31 | keratin 31 | NM_010659.2 | 86.5% | 89.9% | (many diffs) |
| 10 | mouse | 16671 | Krt33b | keratin 33B | NM_013570.1 | 84.5% | 85.3% | (many diffs) |
| 11 | mouse | 71888 | Krt33a | keratin 33A | NM_027983.3 | 84% | 86.7% | (many diffs) |
| 12 | mouse | 16672 | Krt34 | keratin 34 | NM_027563.4 | 80.7% | 83.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1317
- ORF length:
- 1248
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccctacaac ttctgcctgc ccagcctgag ctgccgcacc agctgctcct 121 cccggccctg cgtgcccccc agctgccaca gctgcaccct gcccggggcc tgcaacatcc 181 ccgccaatgt gagcaactgc aactggttct gcgagggctc cttcaatggt agcgagaagg 241 agactatgca gttcctgaac gaccgcctgg ccagctacct ggagaaagtg cgtcagctgg 301 agcgggacaa cgcggagctg gagaacctca tccgggagcg gtctcagcag caggagccct 361 tgctgtgccc cagttaccag tcctatttta agaccattga ggagctccag cagaagatcc 421 tgtgtaccaa gtctgagaat gccaggcttg tggtgcagat cgacaacgcc aagctggctg 481 cggatgattt cagaaccaag taccagaccg agctgtccct gcggcagctg gtggagtcgg 541 acatcaacgg tctgcgcagg atcctggatg agctgaccct gtgcaagtcc gacctggagg 601 cccaggtgga gtccctgaag gaggagctgc tctgcctcaa gagcaaccat gagcaggagg 661 tcaataccct gcgctgccag cttggagacc gcctcaatgt ggaggtggat gctgctccca 721 ctgtggacct gaatcgggtg ctgaacgaga ccaggagtca gtatgaggcc ctggtggaaa 781 ccaaccgcag ggaagtggag caatggttca ccacgcagac cgaggagctg aacaagcagg 841 tggtatccag ctcagagcag ctgcagtcct accaggcgga gaTCATCGAG CTGAGACGCA 901 CAGTCAACGC CCTGGAGATC GAGCTGCAGG CCCAGCACAA CCTGCGAGAC TCTCTGGAAA 961 ACACGCTGAC AGAGAGTGAG GCCCGCTACA GCTCCCAGCT GTCCCAGGTG CAGAGCCTGA 1021 TCACCAACGT GGAGTCCCAG CTGGCGGAGA TCCGCAGTGA CCTGGAGCGG CAGAACCAGG 1081 AGTACCAGGT GCTGCTGGAT GTGCGTGCCC GGCTGGAGTG TGAGATCAAC ACATACCGGA 1141 GCCTGCTGGA GAGCGAGGAC TGCAATCTGC CCAGCAATCC CTGTGCCACG ACCAACGCGT 1201 GCAGCAAGCC CATCGGACCC TGTCTCTCCA ATCCCTGTAC CTCTTGTGTC CCTCCTGCCC 1261 CCTGCACACC CTGTGCCCCA CGCCCCCGCT GTGGGCCCTG CAATTCCTTC GTGCGCTTGC 1321 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GAATTAGCGG GGACGAAACG TTTAGAACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt