Construct: ORF TRCN0000467973
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001058.1_s317c1
- Derived from:
- ccsbBroadEn_03554
- DNA Barcode:
- GCACGAATCGTCGTTATCGTATGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAT10 (55226)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467973
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55226 | NAT10 | N-acetyltransferase 10 | NM_024662.3 | 99.9% | 99.9% | 1381T>C;1686C>T |
| 2 | human | 55226 | NAT10 | N-acetyltransferase 10 | NM_001144030.2 | 92.9% | 92.8% | 0_1ins216;1165T>C;1470C>T |
| 3 | mouse | 98956 | Nat10 | N-acetyltransferase 10 | NM_153126.2 | 88.8% | 95.5% | (many diffs) |
| 4 | mouse | 98956 | Nat10 | N-acetyltransferase 10 | XM_006500455.3 | 76.6% | 80.7% | (many diffs) |
| 5 | mouse | 98956 | Nat10 | N-acetyltransferase 10 | XR_374547.3 | 47.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3141
- ORF length:
- 3075
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca tcggaaaaag gtggataacc gaatccggat tctcattgag aatggagtag 121 ctgagcggca aagatctctc tttgttgtag ttggggatcg aggaaaagat caggtggtaa 181 tacttcatca catgttatcc aaagcaactg tgaaggctcg gccttcagtg ctgtggtgtt 241 ataagaaaga gctggggttt agcagtcacc ggaagaaaag aatgcgacag ctgcagaaga 301 aaataaagaa tggaacactg aacataaagc aggacgaccc ctttgaactc ttcatagcag 361 ccacaaacat tcgctactgc tactacaacg agacccacaa gatcctgggc aataccttcg 421 gcatgtgtgt gctgcaggat tttgaagcct taactccaaa cttgctggcc aggactgtag 481 aaacagtgga aggtggtggg ctagtggtca tcctcctacg gaccatgaac tcactcaagc 541 aattgtacac agtgactatg gatgtgcatt ccaggtacag aactgaggcc catcaggatg 601 tggtgggaag atttaatgaa aggtttattc tgtctctggc ctcttgtaag aagtgtctcg 661 tcattgatga ccagctcaac atcctgccca tctcctccca cgttgccacc atggaggccc 721 tgcctcccca gactccggat gagagtcttg gtccttctga tctggagctg agggagttga 781 aggagagctt gcaggacacc cagcctgtgg gtgtgttggt ggactgctgt aagactctag 841 accaggccaa agctgtcttg aaatttatcg agggcatctc tgaaaagacc ctgaggagta 901 ctgttgcact cacagctgct cgaggacggg gaaaatctgc agccctggga ttggcgattg 961 ctggggcggt ggcatttggg tactccaata tctttgttac ctccccaagc cctgataacc 1021 tccatactct gtttgaattt gtatttaaag gatttgatgc tctgcaatat caggaacatc 1081 tggattatga gattatccag tctctaaatc ctgaatttaa caaagcagtg atcagagtga 1141 atgtatttcg agaacacagg cagactattc agtatataca tcctgcagat gctgtgaagc 1201 tgggccaggc tgaactagtt gtgattgatg aagctgccgc catccccctc cccttggtga 1261 agagcctact tggcccctac cttgttttca tggcatccac catcaatggc tatgagggca 1321 ctggccggtc actgtccctc aagctaattc agcagctccg tcaacagagc gcccagagcc 1381 aggtcagcac cactgctgag aataagacca cgacgacagc cagattggca tcagcgcgga 1441 cactgcatga ggtttccctc caggagtcaa tccgatacgc ccctggggat gcagtggaga 1501 agtggctgaa tgacttgctg tgcctggatt gcctcaacat cactcggata gtctcaggct 1561 gccccttgcc tgaagcttgt gaactgtact atgttaatag agataccctc ttttgctacc 1621 acaaggcctc tgaagttttc ctccaacggc ttatggccct ctacgtggct tctcactaca 1681 agaactctcc caatgatctc cagatgctct ccgatgcacc tgctcaccat ctcttctgcc 1741 ttctgcctcc tgtgcccccc acccagaatg cccttccaga agtgcttgct gttatccagg 1801 tgtgccttga aggggagatt tctcgccagt ccatcttgaa cagtctgtct cgaggcaaga 1861 aggcttcagg ggacctgatt ccatggacag tgtcagaaca gttccaagat ccagactttg 1921 gtggtctgtc tggtggaagg gtcgttcgca ttgctgttca cccagattat caagggatgg 1981 gctatggcag ccgtgctctg cagctgctgc agatgtacta tgaaggcagg tttccttgtc 2041 tggaggaaaa ggtccttgag acaccacagg aaattcacac cgtaagcagc gaggctgtca 2101 gcttgttgga agaggtcatc actccccgga aggacctgcc tcctttactc ctcaaattga 2161 atgagaggcc tgccgaacgc ctggattacc tgggtgtttc ctatggcttg acccccaggc 2221 tcctcaagtt ctggaaacga gctggatttg ttcctgttta tctgagacag accccgaatg 2281 acctgaccgg agagcactcg tgcatcatgc tgaagacgct cactgatgag gatgaggctg 2341 accagggagg ctggcttgca gccttctgga aagatttccg acggcggttc ctagccttgc 2401 tctcctacca gttcagtacc ttctctcctt ccctggctct gaacatcatt cagaacagga 2461 acatggggaa gccagcccag cctgccctga gccgggagga gctggaagca ctcttcctcc 2521 cctatgacct gaagcggctg gagatgtatt cacggaatat ggtggactat cacctcatca 2581 tggacatgat cccggccatc tctcgcatct atttcctgaa ccagctgggg gacctggccc 2641 tgtctgcggc tcagtcggct cttctcttgg ggattggcct gcagcataag tctgtggacc 2701 agctggaaaa ggagattgag ctgccctcgg gccagttgat gggacttttc aaccggatca 2761 TCCGCAAAGT TGTGAAGCTA TTTAATGAAG TTCAGGAAAA GGCCATTGAG GAGCAGATGG 2821 TGGCAGCGAA GGATGTGGTC ATGGAGCCCA CGATGAAGAC CCTCAGTGAC GACCTAGATG 2881 AAGCAGCAAA GGAATTTCAG GAGAAACACA AGAAGGAAGT AGGGAAGCTG AAGAGCATGG 2941 ACCTCTCTGA ATACATAATC CGTGGGGACG ATGAAGAGTG GAATGAAGTT TTGAACAAAG 3001 CTGGGCCGAA CGCCTCGATC ATCAGCCTGA AAAGTGACAA GAAAAGGAAG TTAGAGGCCA 3061 AACAAGAACC CAAACAGAGC AAGAAGTTGA AGAACAGAGA GACAAAGAAC AAAAAAGATA 3121 TGAAACTGAA GCGGAAGAAA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 3181 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 3241 AAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCA CGAATCGTCG 3301 TTATCGTATG GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat 3361 t