Construct: ORF TRCN0000468166
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017376.1_s317c1
- Derived from:
- ccsbBroadEn_14670
- DNA Barcode:
- TACGCAAAGCTATAGTTCATCTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGFG1 (3267)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468166
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | NM_004504.4 | 99.8% | 99.8% | 98A>G;1416A>G |
| 2 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | NM_001135188.1 | 99.5% | 99.4% | 98A>G;1416A>G;1536_1537insGGTGCA |
| 3 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | XM_006712480.3 | 97.1% | 97% | 98A>G;1378_1425del;1464A>G |
| 4 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | XM_005246516.4 | 95.7% | 95.5% | 98A>G;815_886del;1488A>G |
| 5 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | NM_001135187.1 | 95.4% | 95.2% | (many diffs) |
| 6 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | XM_006712478.4 | 93.2% | 93% | (many diffs) |
| 7 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | XM_006712479.4 | 92.9% | 92.6% | (many diffs) |
| 8 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | NM_001135189.1 | 92.7% | 92.7% | 98A>G;691_692ins120;1296A>G |
| 9 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | XM_017003954.1 | 59.9% | 59.4% | (many diffs) |
| 10 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | XM_006712481.3 | 56% | 55.3% | (many diffs) |
| 11 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496433.3 | 92.7% | 96.6% | (many diffs) |
| 12 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496434.3 | 92.5% | 96.6% | (many diffs) |
| 13 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | NM_010472.3 | 92.3% | 96.2% | (many diffs) |
| 14 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496435.3 | 92.2% | 96.2% | (many diffs) |
| 15 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496430.3 | 90.1% | 93.9% | (many diffs) |
| 16 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496431.3 | 90% | 93.9% | (many diffs) |
| 17 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496432.3 | 89.7% | 93.5% | (many diffs) |
| 18 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | NM_001310713.1 | 87.4% | 91.4% | (many diffs) |
| 19 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496438.3 | 87.3% | 91.4% | (many diffs) |
| 20 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496439.3 | 87.1% | 91.1% | (many diffs) |
| 21 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496440.3 | 85.7% | 89.6% | (many diffs) |
| 22 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | NM_001347077.1 | 83.3% | 87.1% | (many diffs) |
| 23 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_017316914.1 | 82.9% | 86.8% | (many diffs) |
| 24 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XR_373537.3 | 56.9% | (many diffs) | |
| 25 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XR_001781822.1 | 18.5% | (many diffs) | |
| 26 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XR_001781825.1 | 18.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1755
- ORF length:
- 1686
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggccagc gcgaagcgga agcaggagga gaagcacctg aagatgctgc 121 gggacatgac cggcctcccg cacaaccgaa agtgcttcga ctgcggccag cgcggcccca 181 cctacgttaa catgacggtc ggctccttcg tgtgtacctc ctgctccggc agcctgcgag 241 gattaaatcc accacacagg gtgaaatcta tctccatgac aacattcaca caacaggaaa 301 ttgaattctt acaaaaacat ggaaatgaag tctgtaaaca gatttggcta ggattatttg 361 atgatagatc ttcagcaatt ccagacttca gggatccaca aaaagtgaaa gagtttctac 421 aagaaaagta tgaaaagaaa agatggtatg tcccgccaga acaagccaaa gtcgtggcat 481 cagttcatgc atctatttca gggtcctctg ccagtagcac aagcagcaca cctgaggtca 541 aaccactgaa atctctttta ggggattctg caccaacact gcacttaaat aagggcacac 601 ctagtcagtc cccagttgta ggtcgttctc aagggcagca gcaggagaag aagcaatttg 661 accttttaag tgatctcggc tcagacatct ttgctgctcc agctcctcag tcaacagcta 721 cagccaattt tgctaacttt gcacatttca acagtcatgc agctcagaat tctgcaaatg 781 cagattttgc aaactttgat gcatttggac agtctagtgg ttcgagtaat tttggaggtt 841 tccccacagc aagtcactct ccttttcagc cccaaactac aggtggaagt gctgcatcag 901 taaatgctaa ttttgctcat tttgataact tccccaaatc ctccagtgct gattttggaa 961 ccttcaatac ttcccagagt catcaaacag catcagctgt tagtaaagtt tcaacgaaca 1021 aagctggttt acagactgca gacaaatatg cagcacttgc taatttagac aatatcttca 1081 gtgccgggca aggtggtgat cagggaagtg gctttgggac cacaggtaaa gctcctgttg 1141 gttctgtggt ttcagttccc agtcagtcaa gtgcatcttc agacaagtat gcagctctgg 1201 cagaactaga cagcgttttc agttctgcag ccacctccag taatgcgtat acttccacaa 1261 gtaatgctag cagcaatgtt tttggaacag tgccagtggt tgcttctgca cagacacagc 1321 ctgcttcatc aagtgtgcct gctccatttg gagctacgcc ttccacaaat ccatttgttg 1381 ctgctgctgg tccTTCTGTG GCATCTTCTA CAAACCCATT TCAGACCAAT GCCAGAGGAG 1441 CAACAGCGGC AACCTTTGGC ACTGCATCCA TGAGCATGCC CACGGGATTC GGCACTCCTG 1501 CTCCCTACAG TCTTCCCACC AGCTTTAGTG GCAGCTTTCA GCAGCCTGCC TTTCCAGCCC 1561 AAGCAGCTTT CCCTCAACAG ACAGCTTTTT CTCAACAGCC CAATGGTGCA GGTTTTGCAG 1621 CATTTGGACA AACAAAGCCA GTAGTAACCC CTTTTGGTCA AGTTGCAGCT GCTGGAGTAT 1681 CTAGTAATCC TTTTATGACT GGTGCACCAA CAGGACAATT TCCAACAGGA AGCTCATCAA 1741 CCAATCCTTT CTTATTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1801 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1861 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TACGCAAAGC TATAGTTCAT 1921 CTCTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt