Construct: ORF TRCN0000468295

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012536.1_s317c1
Derived from:
ccsbBroadEn_13107
DNA Barcode:
CTGACGCTGTAATCCCGACCACGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MSI2 (124540)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468295
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 124540 MSI2 musashi RNA binding protein 2 XM_011524286.2 60.7% 58.2% (many diffs)
2 human 124540 MSI2 musashi RNA binding protein 2 NM_170721.2 60.1% 60.1% 1_300del
3 human 124540 MSI2 musashi RNA binding protein 2 NM_001322251.2 59.2% 59.2% 1_312del
4 human 124540 MSI2 musashi RNA binding protein 2 XM_017024149.2 56.7% 54.4% (many diffs)
5 human 124540 MSI2 musashi RNA binding protein 2 XM_011524287.3 51.9% 43.1% (many diffs)
6 human 124540 MSI2 musashi RNA binding protein 2 XM_017024148.1 49.6% 47.7% (many diffs)
7 human 124540 MSI2 musashi RNA binding protein 2 NM_001322250.1 45.3% 43.5% (many diffs)
8 human 124540 MSI2 musashi RNA binding protein 2 XM_017024147.2 43.1% 38.7% (many diffs)
9 human 124540 MSI2 musashi RNA binding protein 2 NM_138962.4 42.8% 42.9% (many diffs)
10 human 124540 MSI2 musashi RNA binding protein 2 XM_005257014.3 42.4% 40.7% (many diffs)
11 human 124540 MSI2 musashi RNA binding protein 2 XM_005257015.3 42.4% 40.7% (many diffs)
12 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_011249305.2 49.6% 51.2% (many diffs)
13 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_011249304.2 46.8% 48.9% (many diffs)
14 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534442.3 40.5% 42.9% (many diffs)
15 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_017314830.1 39.8% 41.2% (many diffs)
16 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534441.3 38.6% 39.9% (many diffs)
17 mouse 76626 Msi2 musashi RNA-binding protein 2 NM_054043.3 38.4% 40.7% (many diffs)
18 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_011249302.1 37.8% 39.1% (many diffs)
19 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534440.2 37.8% 40% (many diffs)
20 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534438.2 37.2% 38.5% (many diffs)
21 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534439.2 37.2% 38.5% (many diffs)
22 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534434.2 36% 38.1% (many diffs)
23 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534435.2 36% 38.1% (many diffs)
24 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534431.2 35.5% 36.7% (many diffs)
25 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534432.2 35.5% 36.7% (many diffs)
26 mouse 76626 Msi2 musashi RNA-binding protein 2 XR_388550.2 6.1% (many diffs)
27 mouse 76626 Msi2 musashi RNA-binding protein 2 XR_879764.1 6.1% (many diffs)
28 mouse 76626 Msi2 musashi RNA-binding protein 2 XR_001780073.1 6.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
519
ORF length:
453
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt cacaagaaca aagaaaatat ttgtaggcgg gttatctgcg aacacagtag
121tggaagatgt aaagcaatat ttcgagcagt ttggcaaggt gGAAGATGCA ATGCTGATGT
181TTGATAAAAC TACCAACAGG CACAGAGGGT TTGGCTTTGT CACTTTTGAG AATGAAGATG
241TTGTGGAGAA AGTCTGTGAG ATTCATTTCC ATGAAATCAA TAATAAAATG GTAGAATGTA
301AGAAAGCTCA GCCGAAAGAA GTCATGTTCC CACCTGGGAC AAGAGGCCGG GCCCGGGGAC
361TGCCTTACAC CATGGACGCG TTCATGCTTG GCATGGGGAT GCTGGGATAT CCCAACTTCG
421TGGCGACCTA TGGCCGTGGC TACCCCGGAT TTGCTCCAAG CTATGGCTAT CAGTTCCCAG
481ACTATTTGCC GGTTTCACAA GACATAATTT TTATCAACTA CCCAACTTTC TTGTACAAAG
541TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
601GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
661ACGACTGACG CTGTAATCCC GACCACGAAC GCGTTAAGTC gacaatcaac ctctggatta
721caaaatttgt gaaagatt

Download FASTA (ORF) (Full)