Construct: ORF TRCN0000468949
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009942.1_s317c1
- Derived from:
- ccsbBroadEn_01101
- DNA Barcode:
- CACCGCTGGGTGCTAGCGAGCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NME1 (4830)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468949
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_000269.2 | 100% | 100% | |
| 2 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_198175.1 | 85.8% | 85.8% | 1_75del |
| 3 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018137.3 | 81.4% | 88.1% | (many diffs) |
| 4 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018138.1 | 81.4% | 88.1% | (many diffs) |
| 5 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018139.2 | 81.4% | 88.1% | (many diffs) |
| 6 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_002512.4 | 81.4% | 88.1% | (many diffs) |
| 7 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NM_001018136.3 | 53.8% | 50.1% | (many diffs) |
| 8 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NR_037149.2 | 33.8% | (many diffs) | |
| 9 | mouse | 18102 | Nme1 | NME/NM23 nucleoside diphosp... | NM_008704.2 | 87.7% | 94% | (many diffs) |
| 10 | mouse | 18102 | Nme1 | NME/NM23 nucleoside diphosp... | XM_030245646.1 | 80.3% | 86.1% | (many diffs) |
| 11 | mouse | 18103 | Nme2 | NME/NM23 nucleoside diphosp... | NM_001077529.2 | 80% | 88.1% | (many diffs) |
| 12 | mouse | 18103 | Nme2 | NME/NM23 nucleoside diphosp... | NM_008705.5 | 80% | 88.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 522
- ORF length:
- 456
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caactgtgag cgtaccttca ttgcgatcaa accagatggg gtccagcggg 121 gtcttgtggg agagattatc aagcgttttg agcagaaagg attccgcctt gttggtctga 181 aatTCATGCA AGCTTCCGAA GATCTTCTCA AGGAACACTA CGTTGACCTG AAGGACCGTC 241 CATTCTTTGC CGGCCTGGTG AAATACATGC ACTCAGGGCC GGTAGTTGCC ATGGTCTGGG 301 AGGGGCTGAA TGTGGTGAAG ACGGGCCGAG TCATGCTCGG GGAGACCAAC CCTGCAGACT 361 CCAAGCCTGG GACCATCCGT GGAGACTTCT GCATACAAGT TGGCAGGAAC ATTATACATG 421 GCAGTGATTC TGTGGAGAGT GCAGAGAAGG AGATCGGCTT GTGGTTTCAC CCTGAGGAAC 481 TGGTAGATTA CACGAGCTGT GCTCAGAACT GGATCTATGA ATGCCCAACT TTCTTGTACA 541 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 601 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 661 AGGACGACAC CGCTGGGTGC TAGCGAGCCC GACGCGTTAA GTCgacaatc aacctctgga 721 ttacaaaatt tgtgaaagat t