Construct: ORF TRCN0000469162
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005142.1_s317c1
- Derived from:
- ccsbBroadEn_11674
- DNA Barcode:
- GAATGGAGGCACGCGCGGCGGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF1B (23095)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469162
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23095 | KIF1B | kinesin family member 1B | NM_015074.3 | 32.6% | 32.7% | 1_3573del;5163C>A |
| 2 | human | 23095 | KIF1B | kinesin family member 1B | NM_001365951.2 | 31.8% | 31.8% | 1_3711del;5301C>A |
| 3 | human | 23095 | KIF1B | kinesin family member 1B | NM_001365952.1 | 31.8% | 31.8% | 1_3711del;5301C>A |
| 4 | mouse | 16561 | Kif1b | kinesin family member 1B | XM_006538595.3 | 30.4% | 32.7% | (many diffs) |
| 5 | mouse | 16561 | Kif1b | kinesin family member 1B | XM_006538594.3 | 29.7% | 31.9% | (many diffs) |
| 6 | mouse | 16561 | Kif1b | kinesin family member 1B | NM_207682.2 | 29.1% | 31.2% | (many diffs) |
| 7 | mouse | 16561 | Kif1b | kinesin family member 1B | XM_006538593.3 | 29% | 31.1% | (many diffs) |
| 8 | mouse | 16561 | Kif1b | kinesin family member 1B | XM_006538592.3 | 28.7% | 30.9% | (many diffs) |
| 9 | mouse | 16561 | Kif1b | kinesin family member 1B | XM_006538591.3 | 28.5% | 30.7% | (many diffs) |
| 10 | mouse | 16561 | Kif1b | kinesin family member 1B | XM_006538590.3 | 28.4% | 30.6% | (many diffs) |
| 11 | mouse | 16561 | Kif1b | kinesin family member 1B | NM_001290995.1 | 28.3% | 30.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1803
- ORF length:
- 1737
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag caaaaccagc cttggccaga gcatgagcaa gtatgacctc ctggtttggt 121 ttgagatcag tgaactggag cctacaggag agtatatccc agctgtggtt gaccacacag 181 caggcttgcc ttgccagggg acatttttgc ttcatcaggg catccagcga aggatcacag 241 tgaccattat ccatgagaag gggagcgagc tccattggaa agatgttcgt gaactggtgg 301 taggtcgtat tcggaataag cctgaggtgg atgaagctgc agttgatgcc atcctctccc 361 taaatattat ttctgccaag tacctgaagt cttcccacaa ctctagcagg accttctacc 421 gctttgaggc tgtgtgggat agctctctgc ataactccct tcttctgaac cgagtgacac 481 cctatggaga aaagatctac atgaccttgt cggcctacct agagctggat cattgcatcc 541 agccggctgt catcaccaag gatgtgtgca tggtcttcta ctcccgagat gccaagatct 601 caccaccacg ctctctgcgt agcctctttg gcagcggcta ctcaaagtca ccagattcga 661 atcgagtcac tggcatttac gaactcagct tatgcaaaat gtcagacaca ggtagtccag 721 gtatgcagag aaggagaaga aaaatcttag atacgtcagt ggcatatgtg cggggagaag 781 agaacttagc aggctggcgg ccccgtggag acagcctcat ccttgagcac cagtgggagc 841 tggagaagct ggagctccta catgaggtgg aaaaaacccg ccactttttg ctgctgcgtg 901 agagacttgg tgacagcatc cccaaatccc tgagcgactc gttatccccc agcctcagca 961 gtgggaccct cagcacctcc accagtatct cctctcagat ctcaaccact acctttgaaa 1021 gcgccatcac acctagcgag agcagtggct atgattcagg agacatcgaa agcctggtgg 1081 accgagagaa agagctggct accaagtgcc tgcaacttct cacccacact ttcaacagag 1141 aattcagcca ggtgcacggc agcgtcagtg actgtaagtt gtctgatatc tctccaattg 1201 gacgggatcc ctctgagtcc agtttcagca gtgccaccct cactccctcc tccacctgtc 1261 cctctctggt agactctagg agcaactctc tggatcagaa gaccccagaa gccaattccc 1321 gggcctctag tccctgccca gaatttgaac agtttcagat tgtcccagct gtggaaacac 1381 catatttggc ccgagcagga aaaaacgaat ttctcaatct tgttccagat attGAAGAAA 1441 TTAGACCAAG CTCAGTGGTC TCTAAGAAAG GATACCTTCA TTTCAAGGAG CCTCTTTACA 1501 GTAACTGGGC TAAACATTTT GTTGTCGTCC GTCGGCCTTA TGTCTTCATC TATAACAGTG 1561 ACAAAGACCC TGTGGAGCGT GGAATCATTA ACCTGTCCAC AGCACAGGTG GAGTACAGTG 1621 AGGACCAGCA GGCCATGGTG AAGACACCAA ACACATTTGC TGTCTGCACA AAGCACCGTG 1681 GGGTCCTTTT GCAGGCCCTC AATGACAAAG ACATGAACGA CTGGTTGTAT GCCTTCAACC 1741 CACTTCTAGC TGGCACAATA CGGTCAAAGC TTTCCCGCAG ATGCCCGAGC CAGTCGAAAT 1801 ACTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1861 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1921 GGCTTTATAT ATCTTGTGGA AAGGACGAGA ATGGAGGCAC GCGCGGCGGG GAACGCGTTA 1981 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt