Construct: ORF TRCN0000469293
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004427.1_s317c1
- Derived from:
- ccsbBroadEn_00819
- DNA Barcode:
- GCAGACGGAGCGGCCAGCTGGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IDH3B (3420)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469293
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3420 | IDH3B | isocitrate dehydrogenase (N... | NM_006899.5 | 100% | 100% | |
| 2 | human | 3420 | IDH3B | isocitrate dehydrogenase (N... | NM_001330763.2 | 99.3% | 99.4% | 1152_1154delTTC;1157A>G;1159_1161delCTC |
| 3 | human | 3420 | IDH3B | isocitrate dehydrogenase (N... | NM_174855.4 | 95.8% | 96.3% | (many diffs) |
| 4 | human | 3420 | IDH3B | isocitrate dehydrogenase (N... | NM_001258384.2 | 94.2% | 92.9% | (many diffs) |
| 5 | human | 3420 | IDH3B | isocitrate dehydrogenase (N... | XR_001754267.1 | 88.5% | (many diffs) | |
| 6 | human | 3420 | IDH3B | isocitrate dehydrogenase (N... | NR_136344.2 | 80.9% | 1_28del;1184_1427del | |
| 7 | human | 3420 | IDH3B | isocitrate dehydrogenase (N... | XR_001754266.1 | 78.2% | 1_28del;904_905ins38;1146_1390del | |
| 8 | human | 3420 | IDH3B | isocitrate dehydrogenase (N... | XR_001754265.1 | 72.4% | 1_28del;904_905ins38;1146_1504del | |
| 9 | mouse | 170718 | Idh3b | isocitrate dehydrogenase 3 ... | NM_130884.4 | 89.1% | 93.5% | (many diffs) |
| 10 | mouse | 170718 | Idh3b | isocitrate dehydrogenase 3 ... | XM_006498824.3 | 85.9% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1221
- ORF length:
- 1155
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcattgagc ggagtccgct ggctgacccg agcgctggtc tccgccggga 121 accctggggc atggagaggt ctgagtacct cggccgcggc gcacgctgca tcgcggagcc 181 aggccgagga cgtgagggtg gagggctcct ttcccgtgac catgcttccg ggagacggtg 241 tggggcctga gctgatgcac gccgtcaagg aggtgttcaa ggctgccgct gtcccagtgg 301 agttccagga gcaccacctg agtgaggtgc agaatatggc atctgaggag aagctggagc 361 aggtgctgag ttccatgaag gagaacaaag tggccatcat tggaaagatt cataccccga 421 tggagtataa gggggagcta gcctcctatg atatgcggct gaggcgtaag ttggacttat 481 ttgccaacgt agtccatgtg aagtcacttc ctgggtatat gactcggcac aacaatctag 541 acctggtgat cattcgagag cagacagaag gggagtacag ctctctggaa catgagagtg 601 caaggggtgt gattgagtgt ttgaagattg tcacacgagc caagtctcag cggattgcaa 661 agttcgcctt tgactatgcc accaagaagg ggcggggcaa ggtcactgct gtccacaagg 721 ccaacatcat gaaacttggg gatgggttgt tccTGCAGTG CTGTGAGGAA GTTGCTGAAC 781 TGTACCCCAA AATCAAATTT GAGACAATGA TCATAGACAA CTGCTGCATG CAGCTGGTGC 841 AGAATCCTTA CCAGTTTGAT GTGCTTGTGA TGCCCAATCT CTATGGGAAC ATTATTGACA 901 ATCTGGCTGC TGGCCTGGTT GGGGGAGCTG GTGTGGTCCC TGGTGAGAGC TATAGTGCAG 961 AATACGCAGT CTTTGAGACG GGTGCCCGGC ACCCATTTGC CCAGGCAGTG GGCAGGAATA 1021 TAGCCAATCC CACGGCCATG CTGCTGTCGG CTTCCAACAT GCTGCGGCAT CTTAATCTTG 1081 AGTATCACTC CAGCATGATC GCAGATGCGG TGAAGAAGGT GATCAAAGTT GGCAAGGTGC 1141 GGACTCGAGA CATGGGCGGC TACAGCACCA CAACCGACTT CATCAAGTCT GTCATCGGTC 1201 ACCTGCAGAC TAAAGGGAGC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1261 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1321 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCAG ACGGAGCGGC 1381 CAGCTGGTGA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt