Construct: ORF TRCN0000469413

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006602.1_s317c1
Derived from:
ccsbBroadEn_08936
DNA Barcode:
AAACCCGTTCCAAACCATGTGTCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RIC3 (79608)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469413
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79608 RIC3 RIC3 acetylcholine receptor... NM_024557.6 99.6% 99.4% (many diffs)
2 human 79608 RIC3 RIC3 acetylcholine receptor... XM_006718318.4 99.6% 99.4% (many diffs)
3 human 79608 RIC3 RIC3 acetylcholine receptor... NM_001206671.4 99.3% 99.1% (many diffs)
4 human 79608 RIC3 RIC3 acetylcholine receptor... XM_006718317.4 92.3% 92.1% (many diffs)
5 human 79608 RIC3 RIC3 acetylcholine receptor... XM_017018287.2 92.3% 92.1% (many diffs)
6 human 79608 RIC3 RIC3 acetylcholine receptor... NM_001346692.2 86.3% 86.1% (many diffs)
7 human 79608 RIC3 RIC3 acetylcholine receptor... XM_024448684.1 86.3% 86.1% (many diffs)
8 human 79608 RIC3 RIC3 acetylcholine receptor... NM_001346694.2 86% 85.9% (many diffs)
9 human 79608 RIC3 RIC3 acetylcholine receptor... NM_001346693.2 80% 79.8% (many diffs)
10 human 79608 RIC3 RIC3 acetylcholine receptor... NM_001206672.4 77.9% 77.7% (many diffs)
11 human 79608 RIC3 RIC3 acetylcholine receptor... XR_930900.3 63.2% (many diffs)
12 human 79608 RIC3 RIC3 acetylcholine receptor... NM_001346690.2 54.3% 53.8% (many diffs)
13 human 79608 RIC3 RIC3 acetylcholine receptor... NM_001135109.4 50.7% 50.8% 120_121ins543;507C>A
14 human 79608 RIC3 RIC3 acetylcholine receptor... XM_006718321.4 50.4% 45.6% (many diffs)
15 human 79608 RIC3 RIC3 acetylcholine receptor... XM_024448685.1 48.3% 47.2% (many diffs)
16 human 79608 RIC3 RIC3 acetylcholine receptor... XM_024448686.1 48.3% 47.2% (many diffs)
17 human 79608 RIC3 RIC3 acetylcholine receptor... NM_001346691.2 47.4% 46.7% (many diffs)
18 human 79608 RIC3 RIC3 acetylcholine receptor... XR_001747957.2 43.4% (many diffs)
19 human 79608 RIC3 RIC3 acetylcholine receptor... XR_428851.4 42% (many diffs)
20 human 79608 RIC3 RIC3 acetylcholine receptor... NR_144485.2 41.5% (many diffs)
21 human 79608 RIC3 RIC3 acetylcholine receptor... XR_930896.3 41% (many diffs)
22 human 79608 RIC3 RIC3 acetylcholine receptor... XR_002957193.1 36.2% (many diffs)
23 human 79608 RIC3 RIC3 acetylcholine receptor... XR_930897.3 35.2% (many diffs)
24 human 79608 RIC3 RIC3 acetylcholine receptor... XR_930898.3 35% (many diffs)
25 human 79608 RIC3 RIC3 acetylcholine receptor... XR_002957192.1 28.9% (many diffs)
26 human 79608 RIC3 RIC3 acetylcholine receptor... XR_930901.3 28.9% (many diffs)
27 human 79608 RIC3 RIC3 acetylcholine receptor... XR_930903.3 28.4% (many diffs)
28 human 79608 RIC3 RIC3 acetylcholine receptor... XR_428848.4 28.2% (many diffs)
29 human 79608 RIC3 RIC3 acetylcholine receptor... XR_002957194.1 27.8% (many diffs)
30 human 79608 RIC3 RIC3 acetylcholine receptor... XR_002957195.1 25% (many diffs)
31 human 79608 RIC3 RIC3 acetylcholine receptor... XR_930904.3 19.8% (many diffs)
32 human 79608 RIC3 RIC3 acetylcholine receptor... XR_001747959.2 19.8% (many diffs)
33 human 79608 RIC3 RIC3 acetylcholine receptor... XR_930905.3 19.6% (many diffs)
34 human 79608 RIC3 RIC3 acetylcholine receptor... NR_144484.2 17.4% (many diffs)
35 human 79608 RIC3 RIC3 acetylcholine receptor... NR_144497.2 12.2% (many diffs)
36 mouse 320360 Ric3 RIC3 acetylcholine receptor... NM_001311161.1 87% 85.8% (many diffs)
37 mouse 320360 Ric3 RIC3 acetylcholine receptor... NM_001038624.1 86.7% 85.6% (many diffs)
38 mouse 320360 Ric3 RIC3 acetylcholine receptor... XM_017322302.1 54.8% 55.4% (many diffs)
39 mouse 320360 Ric3 RIC3 acetylcholine receptor... NM_178780.3 54.6% 55.2% (many diffs)
40 mouse 320360 Ric3 RIC3 acetylcholine receptor... XM_006507942.2 48.9% 46.6% (many diffs)
41 mouse 320360 Ric3 RIC3 acetylcholine receptor... XM_006507943.3 48.9% 46.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1170
ORF length:
1104
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gtactccaca gtgcagagag tcgctctggc ttctgggctt gtcctggctc
121tgtcgctgct gctgcccaag gccttcctgt cccgcgggaa gcggcaggag ccgccgccga
181cacctgaagg aaaattgggc cgatttccac ctatgatgca tcatcaccag gcacactcag
241atggccagac tcctggggct cgtttccaga ggtctcacct tgccgaggca tttgcaaagg
301ccaaaggatc aggtggaggt gctggaggag gaggtagtgg aagaggtctg atggggcaga
361ttattccgat ctacggtttt gggatttttt tatatatact gtacattcta tttaagctct
421caaaggggaa aacaactgca gaggatggga aatgctatac tgccatgcct ggaaacaccc
481acaggaaaat taccagtttt gagcttgctc aactgcaaga aaaactgaag gagacagaag
541cagccatgga aaaattattc aacagagtgg gacctaatgg tgagagagca cagactgtga
601cttctgacca agagaaacgg ttgctacatc agctccgaga aatcaccagg gtcatgaaag
661aaggaaaatt cattgacaga ttttctccag agaaagaagc tgaggaggcc ccttacatgg
721aggactggga aggTTACCCT GAAGAGACTT ACCCAATTTA TGACCTTTCA GACTGTATCA
781AGCGTAGGCA AGAAACAATC TTGGTGGATT ACCCTGACCC AAAAGAACTT TCTGCTGAAG
841AAATAGCTGA AAGAATGGGA ATGATAGAAG AGGAAGAATC AGATCATTTG GGTTGGGAAA
901GTCTGCCCAC TGACCCCAGA GCCCAGGAAG ATAATTCTGT TACCTCGTGT GATCCAAAGC
961CAGAAACATG TTCCTGCTGT TTTCATGAAG ACGAGGATCC TGCTGTCTTG GCAGAGAATG
1021CTGGATTCAG TGCAGATAGC TACCCTGAGC AAGAGGAAAC CACCAAAGAA GAGTGGTCCC
1081AAGACTTTAA AGATGAAGGG TTGGGCATCA GCACAGATAA AGCATATACA GGCAGCATGC
1141TGAGGAAGCG TAACCCCCAG GGTTTAGAGT GCCCCACTTT CTTGTACAAA GTGGTTGATA
1201TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1261CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAAACC
1321CGTTCCAAAC CATGTGTCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1381tgaaagatt

Download FASTA (ORF) (Full)