Construct: ORF TRCN0000470126
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012175.1_s317c1
- Derived from:
- ccsbBroadEn_09332
- DNA Barcode:
- TGCTAGGGAATCCTAGGAAAAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LMBRD2 (92255)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470126
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92255 | LMBRD2 | LMBR1 domain containing 2 | NM_001007527.2 | 99.9% | 100% | 927G>T |
| 2 | human | 92255 | LMBRD2 | LMBR1 domain containing 2 | XM_011514162.2 | 99.9% | 100% | 927G>T |
| 3 | human | 92255 | LMBRD2 | LMBR1 domain containing 2 | XR_001742340.2 | 67.4% | (many diffs) | |
| 4 | human | 92255 | LMBRD2 | LMBR1 domain containing 2 | XM_017010024.2 | 66.5% | 65.5% | (many diffs) |
| 5 | human | 92255 | LMBRD2 | LMBR1 domain containing 2 | XR_001742339.1 | 25.4% | (many diffs) | |
| 6 | mouse | 320506 | Lmbrd2 | LMBR1 domain containing 2 | NM_177178.3 | 89.2% | 94.5% | (many diffs) |
| 7 | mouse | 320506 | Lmbrd2 | LMBR1 domain containing 2 | XM_006520091.4 | 89.2% | 94.5% | (many diffs) |
| 8 | mouse | 320506 | Lmbrd2 | LMBR1 domain containing 2 | XM_006520092.4 | 72.9% | 77.4% | (many diffs) |
| 9 | mouse | 320506 | Lmbrd2 | LMBR1 domain containing 2 | XR_003951400.1 | 22.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2154
- ORF length:
- 2085
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtggtgca gctttgggac ttgagattgt ttttgtcttt tttctggcat 121 tatttctgct tcatcgatat ggagacttta agaaacagca tagacttgtg attattggaa 181 cactgcttgc ttggtatctc tgctttctta ttgtcttcat actgcctctg gatgttagta 241 cgacaatata caaccggtgc aagcatgctg ctgcaaattc aagccctcct gagaatagca 301 acattacagg attgtacgca actgctaacc ctgttccaag ccagcatcct tgtttcaagc 361 catggagtta cattcctgat ggaatcatgc caattttctg gagggtagtg tattggacgt 421 cacaattttt aacatggatt ctcttacctt ttatgcagtc atatgcaaga tcaggagggt 481 tttccatcac tggaaagatc aaaactgcac taattgagaa tgcaatctac tatggcacct 541 atttgctgat ttttggagca tttttaattt atgtagctgt aaacccacat ttacatttag 601 aatggaacca gcttcagaca attgggatag ctgctgcaaa tacatggggt ctgtttcttc 661 ttgtgttgtt gttggggtat ggcttggtgg aaattcctcg atcatactgg aatggagcaa 721 aaaggggtta tctacttatg aaaacgtatt ttaaggcagc caaactgatg acagagaaag 781 cagatgcaga agagaatttg gaagatgcca tggaggaggt tcgtaaagtg aatgaaagca 841 tcaagtataa tcacccattg agaaaatgtg ttgatacaat acttaaaaag tgccctacag 901 agtatcagga aaaaatgggt aggaacatgg atgattatga agattttgat gaaaagcata 961 gtatctatcc aagtgaaaaa agtctggtaa aacttcataa acaggtgatt tattcagttc 1021 agagacaccg tcgaactcaa gtacaatggc agattctttt ggaacaagca ttttatctag 1081 aagatgtagc aaaaaatgaa actagtgcta ctcatcagtt tgttcacacc tttcaatcgc 1141 cagagccaga aaatcgattt atccaatatt tttataatcc tacatttgaa tggtactggg 1201 aatgtctttt gcgaccatgg ttttacaaga tacttgctgt ggttctgtcc atcttctctg 1261 tgattgttgt gtggtcagag tgcacattct ttagcactac tcctgtctta tccctctttg 1321 cggtcttcat acagctggca gaaaaaacat ataattatat ttatatcgag attgcctgct 1381 tcctttccat cttcttccta agtatctgtg tttattctac tgtgttcagg attcgtgtat 1441 ttaactatta ttatttggcc tcacatcacc agactgatgc ttatagcctt cttttcagtg 1501 gcatgctatt ttgccgtttg acacctcctt tatgtcttaa tttcttgggt ttgacccata 1561 tggattcatc tatctctcac aagaatactc aaccaactgc ttatacatct attatgggtt 1621 ccatgaaagt tttatccttt attgcagatg gattctatat atattatcct atgttggtgg 1681 taattctctg cattgctact tattttagtt tgggaacccg ttgtttgaat ctgctcggtt 1741 tccagcagtt tatgggagat gatgatatga caTCAGACTT AGTTAATGAA GGAAAAGAAT 1801 TAATCAGAAA AGAGAAGAGA AAAAGGCAAA GGCAAGAAGA AGGTGAAAAT CGAAGAAGAG 1861 AATGGAAAGA ACGTTATGGA CACAATAGAG AAGATTCCAC TAGGAACAGA AATATTCATA 1921 CTGACCCCAA AGAGTCAAAC TTCTCAGATG TTAATACCAA CCGTTCTGCA TTCAAATATA 1981 CCAGGGCTAA TAACAGGACT GAAAGGGACC GGATAGAACT TCTCCAAGAT GCAGAACCTT 2041 TGGATTTTAA TGCAGAAACA TTCACTGATG ATCCTCTTGA ATCTGAATCA GGAAGGTATC 2101 AGCCTGGTGG ACGATATCTC TCGATGTCTC GCAGTGACAT ATTTAATGAT GTTTTGCCAA 2161 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2221 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2281 TATCTTGTGG AAAGGACGAT GCTAGGGAAT CCTAGGAAAA ACGACGCGTT AAGTCgacaa 2341 tcaacctctg gattacaaaa tttgtgaaag att