Construct: ORF TRCN0000470199
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007539.1_s317c1
- Derived from:
- ccsbBroadEn_00154
- DNA Barcode:
- GCAAAAATGCCGAGCGGATATGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BCKDHB (594)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470199
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 594 | BCKDHB | branched chain keto acid de... | NM_000056.4 | 100% | 100% | |
| 2 | human | 594 | BCKDHB | branched chain keto acid de... | NM_183050.4 | 100% | 100% | |
| 3 | human | 594 | BCKDHB | branched chain keto acid de... | XM_011536023.3 | 90.8% | 89.5% | (many diffs) |
| 4 | human | 594 | BCKDHB | branched chain keto acid de... | XM_005248756.5 | 90.6% | 87.6% | (many diffs) |
| 5 | human | 594 | BCKDHB | branched chain keto acid de... | XM_011536024.3 | 89% | 87.1% | (many diffs) |
| 6 | human | 594 | BCKDHB | branched chain keto acid de... | XM_011536025.3 | 88.7% | 88.5% | 1040_1043delGTCTinsAGGA;1045C>T;1050_1050delTins127 |
| 7 | human | 594 | BCKDHB | branched chain keto acid de... | NM_001318975.1 | 82.1% | 82.1% | 0_1ins210 |
| 8 | human | 594 | BCKDHB | branched chain keto acid de... | NR_134945.2 | 31.2% | 1_23del;655_748del;1294_3762del | |
| 9 | human | 594 | BCKDHB | branched chain keto acid de... | XR_001743546.2 | 24.3% | (many diffs) | |
| 10 | human | 594 | BCKDHB | branched chain keto acid de... | XR_002956292.1 | 14.8% | (many diffs) | |
| 11 | human | 594 | BCKDHB | branched chain keto acid de... | XR_001743549.2 | 14.6% | (many diffs) | |
| 12 | human | 594 | BCKDHB | branched chain keto acid de... | XR_001743547.2 | 14.6% | (many diffs) | |
| 13 | human | 594 | BCKDHB | branched chain keto acid de... | XR_001743548.2 | 11% | (many diffs) | |
| 14 | mouse | 12040 | Bckdhb | branched chain ketoacid deh... | NM_001305935.1 | 84.9% | 91% | (many diffs) |
| 15 | mouse | 12040 | Bckdhb | branched chain ketoacid deh... | XM_006510786.1 | 74.2% | 79.3% | (many diffs) |
| 16 | mouse | 12040 | Bckdhb | branched chain ketoacid deh... | NM_199195.1 | 72.6% | 79.8% | (many diffs) |
| 17 | mouse | 12040 | Bckdhb | branched chain ketoacid deh... | XM_017313114.1 | 63.1% | 69.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1242
- ORF length:
- 1176
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggttgtagcg gcggctgccg gctggctact caggctcagg gcggcagggg 121 ctgaggggca ctggcgtcgg cttcctggcg cggggctggc gcggggcttt ttgcaccccg 181 ccgcgactgt cgaggatgcg gcccagaggc ggcaggtggc tcattttact ttccagccag 241 atccggagcc ccgggagtac gggcaaactc agaaaatgaa tcttttccag tctgtaacaa 301 gtgccttgga taactcattg gccaaagatc ctactgcagt aatatttggt gaagatgttg 361 cctttggtgg agtctttaga tgcactgttg gcttgcgaga caaatatgga aaagatagag 421 tttttaatac cccattgtgt gaacaaggaa ttgttggatt tggaatcgga attgcggtca 481 ctggagctac tgccattgcg gaaattcagt ttgcagatta tattttccct gcatttgatc 541 agattgttaa tgaagctgcc aagtatcgct atcgctctgg ggatcttttt aactgtggaa 601 gcctcactat ccggtcccct tggggctgtg ttggtcatgg ggctctctat cattctcaga 661 gtcctgaagc attttttgcc cattgcccag gaatcaaggt ggttataccc agaagccctt 721 tccaggccaa aggacttctt ttgtcatgca tagaggataa aaatccttgt atattttttg 781 aacctaaaat actttacagg gcagcagcgg aagaagtccc tatagaacca tacaacatcc 841 cactgtccca ggccgaagtc atacaggaag ggagtgatgt tactctagtt gcctggggCA 901 CTCAGGTTCA TGTGATCCGA GAGGTAGCTT CCATGGCAAA AGAAAAGCTT GGAGTGTCTT 961 GTGAAGTCAT TGATCTGAGG ACTATAATAC CTTGGGATGT GGACACAATT TGTAAGTCTG 1021 TGATCAAAAC AGGGCGACTG CTAATCAGTC ACGAGGCTCC CTTGACAGGC GGCTTTGCAT 1081 CGGAAATCAG CTCTACAGTT CAGGAGGAAT GTTTCTTGAA CCTAGAGGCT CCTATATCAA 1141 GAGTATGTGG TTATGACACA CCATTTCCTC ACATTTTTGA ACCATTCTAC ATCCCAGACA 1201 AATGGAAGTG TTATGATGCC CTTCGAAAAA TGATCAACTA TTGCCCAACT TTCTTGTACA 1261 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1321 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1381 AGGACGAGCA AAAATGCCGA GCGGATATGC CACGCGTTAA GTCgacaatc aacctctgga 1441 ttacaaaatt tgtgaaagat t