Construct: ORF TRCN0000470612
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011924.2_s317c1
- Derived from:
- ccsbBroadEn_08289
- DNA Barcode:
- ACTCCGAGAATTGCACTTTTTGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKAG2 (51422)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470612
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | NM_001040633.1 | 99.8% | 100% | 462G>A;772C>A |
2 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012270.1 | 99.8% | 100% | 462G>A;772C>A |
3 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012271.2 | 99.6% | 99.8% | 462G>A;620_621insAGC;769C>A |
4 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012272.1 | 99.6% | 99.8% | 462G>A;620_621insAGC;769C>A |
5 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_011516283.1 | 92.8% | 92.9% | 1_120del;582G>A;892C>A |
6 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_011516284.1 | 92.6% | 92.7% | (many diffs) |
7 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | NM_016203.4 | 92.1% | 92.2% | 1_132del;594G>A;904C>A |
8 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012269.1 | 91.9% | 92% | (many diffs) |
9 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_005250004.4 | 90.1% | 89.4% | (many diffs) |
10 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_024446786.1 | 90.1% | 89.4% | (many diffs) |
11 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012268.2 | 89.9% | 89.2% | (many diffs) |
12 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_006716021.2 | 84.2% | 83.6% | (many diffs) |
13 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_011516282.1 | 84.1% | 83.4% | (many diffs) |
14 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_005250002.4 | 83.7% | 83.1% | (many diffs) |
15 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | NM_001363698.1 | 80.4% | 79.9% | (many diffs) |
16 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | NM_001304527.1 | 80.2% | 79.7% | (many diffs) |
17 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_005250006.5 | 72.5% | 71.2% | (many diffs) |
18 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | NM_001304531.1 | 62.4% | 62.4% | 0_1ins591;181C>A |
19 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | NM_024429.1 | 62.4% | 62.4% | 0_1ins591;181C>A |
20 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_024446789.1 | 62.4% | 62.4% | 0_1ins591;181C>A |
21 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012276.2 | 62.2% | 62.2% | 0_1ins591;29_30insAGC;178C>A |
22 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012277.2 | 60.7% | 60.5% | (many diffs) |
23 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012278.1 | 58.6% | 58.6% | 0_1ins651;121C>A |
24 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012279.2 | 58.6% | 58.6% | 0_1ins651;121C>A |
25 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012280.2 | 58.6% | 58.6% | 0_1ins651;121C>A |
26 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012281.2 | 58.6% | 58.6% | 0_1ins651;121C>A |
27 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_011516285.2 | 56% | 55.3% | (many diffs) |
28 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_024446787.1 | 56% | 55.3% | (many diffs) |
29 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012274.2 | 55.8% | 55.1% | (many diffs) |
30 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_024446788.1 | 55.8% | 55.1% | (many diffs) |
31 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_011516286.2 | 54.5% | 53.6% | (many diffs) |
32 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_011516287.1 | 52.5% | 51.9% | (many diffs) |
33 | human | 51422 | PRKAG2 | protein kinase AMP-activate... | XM_017012275.2 | 52.5% | 51.9% | (many diffs) |
34 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XM_006535612.3 | 89.6% | 94% | (many diffs) |
35 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XM_006535611.3 | 82.7% | 86.8% | (many diffs) |
36 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | NM_145401.2 | 82.5% | 86.6% | (many diffs) |
37 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | NM_001310480.1 | 74.5% | 75.5% | (many diffs) |
38 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XM_006535614.3 | 71.4% | 74.4% | (many diffs) |
39 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XM_017320595.1 | 70.2% | 73.4% | (many diffs) |
40 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | NM_001170555.1 | 57.2% | 61.5% | (many diffs) |
41 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | NM_001170556.1 | 57% | 61.3% | (many diffs) |
42 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XM_011249740.1 | 56.2% | 60.1% | (many diffs) |
43 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XM_017320596.1 | 56.2% | 60% | (many diffs) |
44 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XR_880821.2 | 37.5% | (many diffs) | |
45 | mouse | 108099 | Prkag2 | protein kinase, AMP-activat... | XR_389738.3 | 36.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1641
- ORF length:
- 1575
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gctcctggac ggagacctgg agggttccgg aaagcattcc tctcgaaagg 121 tggacagccc cttcggcccg ggcagcccct ccaaagggtt cttctccaga ggcccccagc 181 cccggccctc cagccccatg tctgcacctg tgaggcccaa gaccagcccc ggctctccca 241 aaaccgtgtt cccgttctcc taccaggagt ccccgccacg ctcccctcga cgcatgagct 301 tcagtgggat cttccgctcc tcctccaaag agtcttcccc caactccaac cctgctacct 361 cgcccggggg catcaggttt ttctcccgct ccagaaaaac ctccggcctc tcctcctctc 421 cgtcaacacc cacccaagtg accaagcagc acacgtttcc cctggaatcc tataagcacg 481 agcctgaacg gttagagaat cgcatctatg cctcgtcttc ccccccagac acagggcaga 541 ggttctgccc gtcttccttc cagagcccga ccaggcctcc actggcatca ccgacacact 601 atgctccctc caaagccgcg gcgctggcgg cggccctggg acccgcggaa gccggcatgc 661 tggagaagct ggagttcgag gacgaagcag tagaagactc agaaagtggt gtttacatgc 721 gattcatgag gtcacacaag tgttatgaca tcgttccaac cagttcaaag cttgttgtct 781 ttgatactac attacaagtt aaaaaggcct tctttgcttt ggtagccaac ggtgtcagag 841 cagcgccact gtgggagagt aaaaaacaaa gttttgtagg aatgctaaca attacagatt 901 tcataaatat actacataga tactataaat cacctatggt acagatttat gaattagagg 961 aacataaaat tgaaacatgg agggagcttt atttacaaga aacatttaag cctttagtga 1021 atatatctcc agatgcaagc ctcttcgatg ctgtatactc cttgatcaaa aataaaatcc 1081 acagattgcc cgttattgac cctatcagtg ggaatgcact ttatatactt acccacaaaa 1141 gaatcctcaa gttcctccag ctttttatgt ctgatatgcc aaagcctgcc ttcatgaagc 1201 agaacctgga tgagcttgga ataggaacgt accacaacat tgccttcaTA CATCCAGACA 1261 CTCCCATCAT CAAAGCCTTG AACATATTTG TGGAAAGACG AATATCAGCT CTGCCTGTTG 1321 TGGATGAGTC AGGAAAAGTT GTAGATATTT ATTCCAAATT TGATGTAATT AATCTTGCTG 1381 CTGAGAAAAC ATACAATAAC CTAGATATCA CGGTGACCCA GGCCCTTCAG CACCGTTCAC 1441 AGTATTTTGA AGGTGTTGTG AAGTGCAATA AGCTGGAAAT ACTGGAGACC ATCGTGGACA 1501 GAATAGTAAG AGCTGAGGTC CATCGGCTGG TGGTGGTAAA TGAAGCAGAT AGTATTGTGG 1561 GTATTATTTC CCTGTCGGAC ATTCTGCAAG CCCTGATCCT CACACCAGCA GGTGCCAAAC 1621 AAAAGGAGAC AGAAACGGAG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1681 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1741 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACTC CGAGAATTGC 1801 ACTTTTTGAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt