Construct: ORF TRCN0000471296

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010884.1_s317c1
Derived from:
ccsbBroadEn_04568
DNA Barcode:
ATGGGTATTATCCTTACCTTACGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GGTLC1 (92086)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471296
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 92086 GGTLC1 gamma-glutamyltransferase l... NM_178311.3 100% 100%
2 human 92086 GGTLC1 gamma-glutamyltransferase l... NM_178312.3 100% 100%
3 human 92086 GGTLC1 gamma-glutamyltransferase l... XM_005260865.4 100% 100%
4 human 92086 GGTLC1 gamma-glutamyltransferase l... XM_017028126.2 100% 100%
5 human 728226 GGTLC3 gamma-glutamyltransferase l... NM_001355479.1 95.5% 89.3% (many diffs)
6 human 728441 GGT2 gamma-glutamyltransferase 2 XM_011530621.2 95.5% 88.8% (many diffs)
7 human 728441 GGT2 gamma-glutamyltransferase 2 XM_017029185.1 95.5% 88.8% (many diffs)
8 human 91227 GGTLC2 gamma-glutamyltransferase l... NM_199127.2 90.7% 87.1% (many diffs)
9 human 91227 GGTLC2 gamma-glutamyltransferase l... XM_024452301.1 90.7% 87.1% (many diffs)
10 human 92086 GGTLC1 gamma-glutamyltransferase l... XM_005260863.4 89.2% 89.2% 417_497del
11 human 92086 GGTLC1 gamma-glutamyltransferase l... XM_005260864.4 89.2% 89.2% 417_497del
12 human 92086 GGTLC1 gamma-glutamyltransferase l... XM_011529391.3 89.2% 89.2% 417_497del
13 human 92086 GGTLC1 gamma-glutamyltransferase l... XM_011529392.2 86.6% 86.6% 0_1ins90
14 human 92086 GGTLC1 gamma-glutamyltransferase l... XM_011529393.2 86.6% 86.6% 0_1ins90
15 human 91227 GGTLC2 gamma-glutamyltransferase l... NM_001282879.1 85.7% 84.1% (many diffs)
16 human 91227 GGTLC2 gamma-glutamyltransferase l... XM_005261817.3 85.7% 84.1% (many diffs)
17 human 91227 GGTLC2 gamma-glutamyltransferase l... XM_011530497.1 85.7% 84.1% (many diffs)
18 human 102724197 LOC102724197 inactive glutathione hydrol... XM_006724929.2 85.4% 79.7% (many diffs)
19 human 728226 GGTLC3 gamma-glutamyltransferase l... XM_024452264.1 85.3% 79.7% (many diffs)
20 human 728441 GGT2 gamma-glutamyltransferase 2 XM_006724396.2 85.3% 79.3% (many diffs)
21 human 728441 GGT2 gamma-glutamyltransferase 2 XM_017029184.1 85.3% 79.3% (many diffs)
22 human 91227 GGTLC2 gamma-glutamyltransferase l... XM_005261818.2 82.3% 79% (many diffs)
23 human 91227 GGTLC2 gamma-glutamyltransferase l... XM_005261815.3 78.5% 77% (many diffs)
24 human 102724197 LOC102724197 inactive glutathione hydrol... XM_006724928.3 67.1% 62.6% (many diffs)
25 human 728441 GGT2 gamma-glutamyltransferase 2 XM_005261890.4 67% 62.3% (many diffs)
26 human 728441 GGT2 gamma-glutamyltransferase 2 XM_017029183.1 67% 62.3% (many diffs)
27 human 102724197 LOC102724197 inactive glutathione hydrol... XM_017030145.2 58% 54.1% (many diffs)
28 human 728441 GGT2 gamma-glutamyltransferase 2 XM_006724395.4 53.3% 49.6% (many diffs)
29 human 728441 GGT2 gamma-glutamyltransferase 2 XM_017029182.2 52.2% 48.5% (many diffs)
30 human 102724197 LOC102724197 inactive glutathione hydrol... XM_006724924.4 49% 45.7% (many diffs)
31 human 102724197 LOC102724197 inactive glutathione hydrol... XM_017030144.1 49% 45.7% (many diffs)
32 human 728441 GGT2 gamma-glutamyltransferase 2 XM_006724392.3 49% 45.5% (many diffs)
33 human 728441 GGT2 gamma-glutamyltransferase 2 XM_017029179.2 49% 45.5% (many diffs)
34 human 728441 GGT2 gamma-glutamyltransferase 2 XM_017029180.1 49% 45.5% (many diffs)
35 human 728441 GGT2 gamma-glutamyltransferase 2 XM_017029181.2 49% 45.5% (many diffs)
36 human 102724197 LOC102724197 inactive glutathione hydrol... XM_017030143.2 43.9% 41% (many diffs)
37 human 102724197 LOC102724197 inactive glutathione hydrol... XM_006724921.4 41.6% 38.8% (many diffs)
38 human 102724197 LOC102724197 inactive glutathione hydrol... XM_017030136.2 41.6% 38.8% (many diffs)
39 human 102724197 LOC102724197 inactive glutathione hydrol... XM_017030137.2 41.6% 38.8% (many diffs)
40 human 102724197 LOC102724197 inactive glutathione hydrol... XM_017030140.2 41.6% 38.8% (many diffs)
41 human 102724197 LOC102724197 inactive glutathione hydrol... XM_017030141.2 41.6% 38.8% (many diffs)
42 human 102724197 LOC102724197 inactive glutathione hydrol... XM_017030142.2 41.6% 38.8% (many diffs)
43 human 2678 GGT1 gamma-glutamyltransferase 1 NM_001288833.2 38.4% 37.9% (many diffs)
44 human 2678 GGT1 gamma-glutamyltransferase 1 NM_013421.3 38.4% 37.9% (many diffs)
45 human 2678 GGT1 gamma-glutamyltransferase 1 NM_013430.2 38.4% 37.9% (many diffs)
46 human 102724197 LOC102724197 inactive glutathione hydrol... XM_011546256.3 37.8% 35.3% (many diffs)
47 human 728441 GGT2 gamma-glutamyltransferase 2 NM_001351304.1 37.4% 34.8% (many diffs)
48 human 728441 GGT2 gamma-glutamyltransferase 2 XM_017029186.2 37.4% 34.8% (many diffs)
49 human 2679 GGT3P gamma-glutamyltransferase 3... NR_003267.1 31.7% (many diffs)
50 human 102724823 LOC102724823 inactive glutathione hydrol... XR_002958856.1 17.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
741
ORF length:
675
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac ctccgagttc ttctctgccc agctccgggc ccagatctct gacgacacca
121ctcacccgat ctcctactac aagcccgagt tctacatgcc ggatgacggg ggcactgctc
181acctgtctgt ggtcgcagag gacggcagtg ctgtgtccgc caccagcacc atcaacctct
241actttggctc caaggtgcgc tccccagtca gcgggatccT GCTCAATAAT GAAATGGATG
301ACTTCAGCTC TACCAGCATC ACCAACGAGT TTGGGGTACC CCCCTCACCT GCCAATTTCA
361TCCAGCCAGG GAAGCAGCCG CTCTCGTCCA TGTGCCCGAC GATCATGGTG GGCCAGGACG
421GCCAGGTCCG GATGGTGGTG GGAGCTGCCG GGGGCACGCA GATCACCATG GCCACTGCAC
481TGGCCATCAT CTACAACCTC TGGTTCGGCT ATGACGTGAA GTGGGCCGTG GAGGAGCCCC
541GGCTGCACAA CCAGCTTCTG CCCAACGTCA CGACAGTGGA GAGAAACATT GACCAGGAAG
601TGACTGCAGC CCTGGAGACC CGGCACCATC ACACCCAGAT CACGTCCACC TTCATTGCTG
661TGGTGCAAGC CATCGTCCGC ATGGCTGGTG GCTGGGCAGC TGCCTCGGAC TCCAGGAAAG
721GTGGGGAACC TGCTGGCTAC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
781CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
841AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATGG GTATTATCCT
901TACCTTACGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)