Transcript: Human NM_013421.3

Homo sapiens gamma-glutamyltransferase 1 (GGT1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-07-21
Taxon:
Homo sapiens (human)
Gene:
GGT1 (2678)
Length:
2558
CDS:
692..2401

Additional Resources:

NCBI RefSeq record:
NM_013421.3
NBCI Gene record:
GGT1 (2678)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_013421.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000036289 CTACAACAGCACCACACGAAA pLKO.1 970 CDS 100% 4.950 3.465 N GGT1 n/a
2 TRCN0000036291 TGCTCGAAGATTGGGAGGGAT pLKO.1 839 CDS 100% 2.640 1.848 N GGT1 n/a
3 TRCN0000036290 CCCAGCATCACCAACGAGTTT pLKO.1 1970 CDS 100% 4.950 2.970 N GGT1 n/a
4 TRCN0000286774 CCCAGCATCACCAACGAGTTT pLKO_005 1970 CDS 100% 4.950 2.970 N GGT1 n/a
5 TRCN0000036292 CAATAATGAAATGGACGACTT pLKO.1 1942 CDS 100% 4.050 2.430 N GGT1 n/a
6 TRCN0000365286 CCAGCATCACCAACGAGTTTG pLKO_005 1971 CDS 100% 10.800 5.400 Y GGTLC1 n/a
7 TRCN0000370351 GCACCATCAACCTCTACTTTG pLKO_005 1884 CDS 100% 10.800 5.400 Y GGTLC1 n/a
8 TRCN0000294177 TCATCCTCAACATCCTCAAAG pLKO_005 1554 CDS 100% 10.800 5.400 Y GGT1 n/a
9 TRCN0000306949 GGACAAGGCTGACAAGCAATC pLKO_005 2413 3UTR 100% 6.000 3.000 Y GGT1 n/a
10 TRCN0000034518 AGCACCATCAACCTCTACTTT pLKO.1 1883 CDS 100% 5.625 2.813 Y GGT3P n/a
11 TRCN0000370352 CCCTCACCTGCCAATTTCATC pLKO_005 2000 CDS 100% 4.950 2.475 Y GGTLC1 n/a
12 TRCN0000036293 CCTCTTCCTCACCATCTACAA pLKO.1 955 CDS 100% 4.950 2.475 Y GGT1 n/a
13 TRCN0000286775 CCTCTTCCTCACCATCTACAA pLKO_005 955 CDS 100% 4.950 2.475 Y GGT1 n/a
14 TRCN0000370353 CTCACCCGATCTCCTACTACA pLKO_005 1779 CDS 100% 4.950 2.475 Y GGTLC1 n/a
15 TRCN0000118426 TCACCCGATCTCCTACTACAA pLKO.1 1780 CDS 100% 4.950 2.475 Y LOC440819 n/a
16 TRCN0000034515 CCTCATCCTCAACATCCTCAA pLKO.1 1552 CDS 100% 4.050 2.025 Y GGT3P n/a
17 TRCN0000034517 GCCTGTCTTGTGTGAGGTGTT pLKO.1 1255 CDS 100% 4.050 2.025 Y GGT3P n/a
18 TRCN0000035773 CATCATCTACAACCTCTGGTT pLKO.1 2143 CDS 100% 2.640 1.320 Y GGTLC1 n/a
19 TRCN0000034516 CCCAAGTTTGTGGATGTGACT pLKO.1 1688 CDS 100% 2.640 1.320 Y GGT3P n/a
20 TRCN0000118482 CCAGGAGGACAAGGCTGACAA pLKO.1 2407 3UTR 100% 1.650 0.825 Y GGTLC2 n/a
21 TRCN0000035770 GAGAGAAACATTGACCAGGAA pLKO.1 2237 CDS 100% 2.640 1.320 Y GGTLC1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_013421.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15427 pDONR223 0% 39.5% 39.5% None 1_1032del n/a
2 ccsbBroad304_15427 pLX_304 0% 39.5% 39.5% V5 1_1032del n/a
3 TRCN0000473857 CTTATTAAATCCAATATGGTTCCG pLX_317 57.3% 39.5% 39.5% V5 1_1032del n/a
4 ccsbBroadEn_10846 pDONR223 100% 39.4% 39.5% None 1_1032del;1041C>T n/a
5 ccsbBroad304_10846 pLX_304 0% 39.4% 39.5% V5 1_1032del;1041C>T n/a
6 TRCN0000470995 TTTTATAAGTCTGACATTTAGAAT pLX_317 58.5% 39.4% 39.5% V5 1_1032del;1041C>T n/a
7 ccsbBroadEn_15426 pDONR223 0% 39.3% 39.1% None (many diffs) n/a
8 ccsbBroad304_15426 pLX_304 0% 39.3% 39.1% V5 (many diffs) n/a
9 TRCN0000468580 CTGTGCATCTCCCGATGGCTGTAC pLX_317 54.6% 39.3% 39% V5 (many diffs) n/a
10 ccsbBroadEn_04568 pDONR223 100% 38.4% 37.9% None (many diffs) n/a
11 ccsbBroad304_04568 pLX_304 0% 38.4% 37.9% V5 (many diffs) n/a
12 TRCN0000471296 ATGGGTATTATCCTTACCTTACGT pLX_317 67.9% 38.4% 37.9% V5 (many diffs) n/a
13 ccsbBroadEn_09311 pDONR223 100% 37.1% 35.8% None (many diffs) n/a
14 TRCN0000480852 CCGCTCGGTCCTACGGAGCGTTCA pLX_317 59% 37.1% 35.8% V5 (many diffs) n/a
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