Transcript: Human XM_011529393.2

PREDICTED: Homo sapiens gamma-glutamyltransferase light chain 1 (GGTLC1), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GGTLC1 (92086)
Length:
1340
CDS:
590..1177

Additional Resources:

NCBI RefSeq record:
XM_011529393.2
NBCI Gene record:
GGTLC1 (92086)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011529393.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000365293 AGCGGGATCCTGCTCAATAAT pLKO_005 704 CDS 100% 15.000 9.000 N GGTLC1 n/a
2 TRCN0000365226 AGATCACGTCCACCTTCATTG pLKO_005 1071 CDS 100% 10.800 6.480 N GGTLC1 n/a
3 TRCN0000035769 ACCAGCATCACCAACGAGTTT pLKO.1 746 CDS 100% 4.950 2.970 N GGTLC1 n/a
4 TRCN0000365292 CGGACAAGGCTGACAAGCAAT pLKO_005 1188 3UTR 100% 4.950 2.970 N GGTLC1 n/a
5 TRCN0000035772 CTGCTCAATAATGAAATGGAT pLKO.1 713 CDS 100% 3.000 1.800 N GGTLC1 n/a
6 TRCN0000035771 CCCGAGTTCTACATGCCGGAT pLKO.1 578 5UTR 100% 0.720 0.432 N GGTLC1 n/a
7 TRCN0000365286 CCAGCATCACCAACGAGTTTG pLKO_005 747 CDS 100% 10.800 5.400 Y GGTLC1 n/a
8 TRCN0000365285 GAACCTGCTGGCTACTGATTG pLKO_005 1160 CDS 100% 10.800 5.400 Y GGTLC1 n/a
9 TRCN0000370351 GCACCATCAACCTCTACTTTG pLKO_005 660 CDS 100% 10.800 5.400 Y GGTLC1 n/a
10 TRCN0000306949 GGACAAGGCTGACAAGCAATC pLKO_005 1189 3UTR 100% 6.000 3.000 Y GGT1 n/a
11 TRCN0000034518 AGCACCATCAACCTCTACTTT pLKO.1 659 CDS 100% 5.625 2.813 Y GGT3P n/a
12 TRCN0000370352 CCCTCACCTGCCAATTTCATC pLKO_005 776 CDS 100% 4.950 2.475 Y GGTLC1 n/a
13 TRCN0000370353 CTCACCCGATCTCCTACTACA pLKO_005 555 5UTR 100% 4.950 2.475 Y GGTLC1 n/a
14 TRCN0000118426 TCACCCGATCTCCTACTACAA pLKO.1 556 5UTR 100% 4.950 2.475 Y LOC440819 n/a
15 TRCN0000035773 CATCATCTACAACCTCTGGTT pLKO.1 919 CDS 100% 2.640 1.320 Y GGTLC1 n/a
16 TRCN0000035770 GAGAGAAACATTGACCAGGAA pLKO.1 1013 CDS 100% 2.640 1.320 Y GGTLC1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011529393.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04568 pDONR223 100% 86.6% 86.6% None 0_1ins90 n/a
2 ccsbBroad304_04568 pLX_304 0% 86.6% 86.6% V5 0_1ins90 n/a
3 TRCN0000471296 ATGGGTATTATCCTTACCTTACGT pLX_317 67.9% 86.6% 86.6% V5 0_1ins90 n/a
4 ccsbBroadEn_15427 pDONR223 0% 84% 83.1% None (many diffs) n/a
5 ccsbBroad304_15427 pLX_304 0% 84% 83.1% V5 (many diffs) n/a
6 TRCN0000473857 CTTATTAAATCCAATATGGTTCCG pLX_317 57.3% 84% 83.1% V5 (many diffs) n/a
7 ccsbBroadEn_10846 pDONR223 100% 84% 83.1% None (many diffs) n/a
8 ccsbBroad304_10846 pLX_304 0% 84% 83.1% V5 (many diffs) n/a
9 TRCN0000470995 TTTTATAAGTCTGACATTTAGAAT pLX_317 58.5% 84% 83.1% V5 (many diffs) n/a
10 ccsbBroadEn_15426 pDONR223 0% 83.7% 82.2% None (many diffs) n/a
11 ccsbBroad304_15426 pLX_304 0% 83.7% 82.2% V5 (many diffs) n/a
12 TRCN0000468580 CTGTGCATCTCCCGATGGCTGTAC pLX_317 54.6% 83.5% 81.7% V5 (many diffs) n/a
13 ccsbBroadEn_09311 pDONR223 100% 77.9% 74.6% None (many diffs) n/a
14 TRCN0000480852 CCGCTCGGTCCTACGGAGCGTTCA pLX_317 59% 77.9% 74.6% V5 (many diffs) n/a
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